中國野生菰谷蛋白基因分子克隆及序列分析.pdf

1、東南大學(xué)碩士學(xué)位論文中國野生菰谷蛋白基因分子克隆及序列分析姓名：郭寶福申請學(xué)位級別：碩士專業(yè)：營養(yǎng)與食品衛(wèi)生學(xué)指導(dǎo)教師：翟成凱20060716東南大學(xué)顧I學(xué)位論義CloningandSequenceAnalysisofGenesRelatedtoGlutelininWildRiceAbstractSchoolofPublicHealth，SoutheastUniversityGU0Bao—fuSupervisor：ZHAIChengka

2、iObjective：ToclonecDNAencodingglutelinfromwildrice(ZizanialatifoliaTurcz)acquirethepropertyrightofthegenes，establishthefoundationoffunctionstudyrelatedtoglutelininwildrice，probeintoevolutionbetweenwildriceandricebycompar

3、isonwiththesequenceofglutelin，enrichthegenepoolofriceanddofavortothecharactersimprovingofriceMethods：(1)ACTAB—LiCIbasedextractionmethodwhichisespeciallyusefulfortheRNAextractionofplantmaterialplentyofpolysaccharide，hydro

4、xybenzeneandlipidsisimprovedExtracttotalRNAofwildriceembⅣosamplesthroughthismethod(2)DesigntheprimersbasedonconservedsequenceofriceglutelingenesreportedpreviouslyandtwopartialcDNAsequencesofwildriceglutelingenesnamedGlu一

5、1andGlu2isobtainedfromRTPCR(3)Designgenespecificprimersbasedonthetwospecificfragmentsandthe5’endand3endsequencesofglu1andglu2areobtainedf『omRLM—RACE(4)BothGlu一1andGlu2completecDNAsequenceareobtainedbyoverlapping5’RACEand

6、3’RACEproductsandanalyzedwithbioinformaticsResults：(1)HighqualitytotalRNAofwildriceemb吖osamplesisobtained(2)ThelengthofspecificfragmentsobtainedfromRTPCRare1381bp(Glu1)and1279bp(Glu一2)BLASTresearchwithdatabase(wwwncbinlm

7、nihgov)showsthat’theyarehighlyhomogenouswithriceglutelingenes(3)The5’endand3’endsequencesofGlu1andGlu2areobtainedfromRLM—RACEThe5’RACEand3’RACEproductssizeofGlu1are433bpand358bprespectively，andGlu1are447bpand695bprespect