《生物信息學(xué)》實驗教學(xué)安排

1、ExperimentsinBioinfmatics(《生物信息學(xué)》實驗指導(dǎo))ExperimentsinBioinfmaticsEditedbyLongjiangFanExperiment1.ConstructionofGeicMapsExperiment2.AnalysisofDNASequenceExperiment3.AnalysisofProteinSequenceExperiment4.MultipleSequenceAlign

2、mentsExperiment5.AnalysisofGeneFunctionLastupdated:2002116ExperimentsinBioinfmatics(《生物信息學(xué)》實驗指導(dǎo))C.J.BastonZ.B.ZengMAPMAKERQTLVersion1.1byStephenE.LincolnMarkJ.DalyEricS.LerQTLMapperVersion1.0byDaolongWangJunZhuetal.PROCE

3、DUREDownloadsetupthesoftware(WinQTLCartQTLMapper)datafilesthisguideThefileslistfthisexperiment:WinQTLCartQTLMapperBioinfmaticsguide.docriceQTL.mapriceQTL.txtMletest.mapMletest.txtDownloadthosefilefromBioinplantLabpage(ww

4、w.cab.zju.bioinplanttool)MappingQTLsbasedonIMCIMwithWinQTLCartStep1.StartbyinputingcreatingsourcedataverifythedataOpenverifyMletest.mcdfile.Step2.ViewmodifythesourcedataRecognizeitsdatabodyStep3.“Intervalmapping”iteminME

5、THODmenufthesourcedataanalysisTheresultfilemletestI.qrtiscreated.Step4.“CompositeIntervalmapping”iteminMETHODmenufthesourcedataanalysisTheresultfilemletestC.qrtiscreated.Step5.Viewthemappingresultsingraphicscomparetheres

6、ultsOpenmletestI.qrtmletestC.qrtrespectivelycomparetheirLRLODgraphs.MappingQTLsbasedonMCIMwithQTLMapperStep1.StartbyknowingmenusystemofQTLMapper1.0Step2.PreparinginputfilesTouseQTLMapper1.0fQTLmappinganalysisyouneedtoget

7、yourmarkerlinkagemapdataofmarkerstraitsintotwoplaintextfiles(amapfileadatafile)inafmatrecognizedbyQTLMapper1.0.ThesefilesarecollectivelycalledInputFiles.Step3.Wkingwith“File”submenuFilesubmenuperfmsoperationsrelatedtoinp

8、utfiles.Openthemapfile(mletest.map)thedatafile(mletst.txt)respectively.Step4.Wkingwith“Run”submenuRunsubmenuimplementsalltheoperationsrelatedtomappingQTLs.Somesuggestions:“2.MapmaineffectQTL”torunChangethe“Genomicrange”o