二至丸的定性定量測定

1、The qualitative analysis and quantitative analysisof Erzhi pillPurpose1. To master the qualitative analysis of composing drugs of Chinese traditional patent medicine by thin layer chromatogram.2. To grasp the application

2、 of high performance liquid chromatogram in quantitative analysis of traditional Chinese patent medicine.Part 1. Qualitative AnalysisCondition1. Apparatus: Ultrasonic cleaner, ultraviolate analyzer2. Reagents: Erzhi pill

3、, diethyl ether, ethyl, Oleanolic acid CRS, ethanol, chloroform, methanol, phosphomolybdic acid, and etc.Procedure1. Preparation of the test solution and the reference solutionTo 0.5g of the powder add 30ml of diethyl et

4、her, heat under reflux for 40mins, and filter. Evaporate the filtrate to dryness, and dissolve the residue in 1ml of ethyl as the test solution. Dissolve Oleanolic acid CRS in ethyl alcohol to prepare a solution containi

5、ng 1mg per ml as the reference solution.2. TLC procedureCarry out the method for thin layer chromatography, using silica gel G as the coating substance and chloroform and methanol (40:1) as the mobile phase. Apply separa

6、tely 5μl of each of the two solutions to the plate. After developing and removal of the plate, dry in air, spray with phosphomolybdic acid and heat to the spots clear.① Part 2. Quantitative AnalysisCondition1. Appara

7、tus: Ultrasonic cleaner2. Reagents: Erzhi pill, methanol, specnuezhenide CRS, wedelolactone CRS, acetonitrile and 0. 1% phosphoric acid solution and etc.Procedure1. Preparation of the reference solutionThe reference solu

8、tion is prepared by dissolving a quantity of specnuezhenide CRS and wedelolactone CRS in methanol to produce a solution. The content of specnuezhenide CRS is 0.8mg/ml. The content of wedelolactone CRS is 0.1 mg/mg.2. Pre

9、paration of the test solution 1.0g of the sample is weighed and contained in a 25ml volumetric flask. Add accurately 20ml of methanol to the flask, extract for 45mins in an ultrasonic cleaner. After that, take it out and

10、 allow it to cool. Add methanol to the mask of the volumetric flask. Shake it well and filter. After filtration, the successive filtrate was further filtered with Millipore membrane (particle size 1.45μm). thus, the succ

11、essive filtrate is used as the test solution.3. Quantitative analysis conditionsColumn: DIKMA C18 ( 4. 6 mm×200 mm，5μm) Mobile phase: acetonitrile and 0. 1% phosphoric acid solutionFlow rate: 0.1ml/min

12、 Wavelength of detector: 215nm①4. 20μl of test solution is injected accurately into the column, and the content of the sample is determined accordin

13、g to one point external standard method.① Chinese Pharmacopoeia 2010 ① HE Mu, YAO Weifeng, ZHANG Li, DING Anwei, Simultaneous determination of four compounds in Erzhiwan by HPLC, China journal of Chinese Materia Medica,