簡介：合肥工業(yè)大學碩士學位論文淠史杭灌區(qū)信息化管理總體構想與初步設計姓名李宗堯申請學位級別碩士專業(yè)水利工程指導教師金菊良張永江20050401INFORMATIONADMINISTRATIONSYSTEMDESJGNOFPISHJHANGIRRIGATIONAREAINANHUIPROVINCEABSTRACTWIMT王IEDEVELOPMENTOFCOMPUTERA11DINTEMETTECHNOLOGYBYLE印SAILDBOUNDS，IRSIMPONANTTOBUNDIRRIGATIONAREAINFBRNLATIONSYSTEM，WHICHHASPCRFECT‰嘶ON，RUNSRELIABLY’USESCCONOMICAILYCONNECTEDCLOSELYWITHTHEREALITYOFPISHIH趴GIRRIGATIONAREAINA1111UIPROVINCEANDFACEDTOTHEGENERALDEMANDOFWALERCONSERVANCYMANAGEMENTDEPARBTLENT，THISP印ERUSEDW曲TEC王LILOLOGY，DATABASEA11DOMERADVAILCEDCOMPUTERTECHNOLOGYTOBUILDWATERCONSERV姐CYGOVEMMENTAFFAIRSMANAGEMENTINFORMATIONSYSTEM，INFONNATIONNETWORKNATBUIIDINGOFIRRIGATIONAREA，WATERMANAGEMENTDECISIONSUPPORTSYS把MATMESAMETIME，THISPAPERCOMBINESMEREASONABLEDISTRIBUTIONOFWATERRESOURCES州THSOCIALECONOMYDEVELOPMENTFROMT11E蛐91EOFSYS捃MAJLALYSIS，OPTIMIZATJONDJS仃IBUTIONJSPRESENTED。THISHASSIGN灑CAILCESFORFUMLLINGTLLESTRATCGYOFECONOMYDEVELOPMENTINANHUIPMVINCEBASED0NPLENTYOFSTUDYING，THISPAPERFUL6LSTHE‰CTIONOFINPUUING趾DOUTPLJHING，QUEF血G，F(xiàn)0酋ECASTINGA11DDISPATCHINGITCANIMPROVCTHCLEVELOFIRRIGATIONAREAMANAGEMEMAIIDHASHIGHREFBRCNCEVALUEFORDEVELOPINGOTHERWATERCONSERVANCYMAIIAGEMENTSYGTEMKEYWORDSPISHIHALLGIRRIGATIONMEA；M鋤AGEMENTINF_OMATIZATION；DECISIONSUPPORTSYSTEM；INFBRNLADONMALLAGEMENTSYSTEM

簡介：安徽農(nóng)業(yè)大學碩士學位論文淠史杭灌區(qū)水稻節(jié)水灌溉專家系統(tǒng)的研究姓名李佳新申請學位級別碩士專業(yè)作物栽培學與耕作學指導教師黃義德王儒敬20050501ABSTRACTSTARTING、ⅣITHTHEBASICPRINCIPLESOFWATERSAVINGIRRIGATIONWSD，THEAUTHORANALYZEDEACHPARAMETEROFIRRIGATIONFORECASTANDPOSEDTHEBASISOFWSIFORECAST，THEEXPERTSYSTEMFORWATERSAVINGIRRIGATIONOFRICEINPISHIHANGIRRIGATIONDISTRICTWASDESIGNEDBYAGRICULTURALINFORMATIONPLATFORM1OAIPL0DEVELOPEDBYHEIFEIINSTITUTEOFINTELLIGENTMACHINES，ACADEMYSCIENCESOFCHINATHISSYSTEMWASDESIGNEDWITHBOTHTHEBASEOFEXPELS’KNOWLEDGEANDYEARSOFEXPERIENCEINRICEPRODUCTIONANDCOMPUTERSOFTWARE，ALSOTHEUTILIZATIONOFKNOWLEDGEENGINEERINGANDINFORMATIONTECHNOLOGYTHEORGANIZATIONANDSTRUCTUREOFTHISDESIGNEDSYSTEMINCLUDESSIXMAINPACTSKNOWLEDGEBASE，DATABANK，MODELBASE，HUMANCOMPUTERINTERFACE，EXPLANATIONUNIT，ANDREASONINGUNITTHEKNOWLEDGEBASEANDREASONINGUNITAREMUTUALLYINDEPENDENTAND、ⅣITLLCONSIDERABLYSTRONGCAPACITYOFEXTENSION；THEMODULESTRUCTUREENHANCESTHESYSTEMTOBEFURTHEREXTENSIONANDMOREPERFECTONLYBYTHEEXTENSIONANDPERFECTIONOFTHESPECIFICMODULE，F(xiàn)URTHERMORE，THISSYSTEM，SYNTHETICALLYINTEGRATED麗THAGRICULTURALEXPERTSYSTEM，DECISIONSUPPORTSYSTEM，ASWELLASMULTIMEDIATECHNOLOGYISNOTONLYOBJECTIVE，IMAGINABLE，ANDFRIENDLYFORHUMANCOMPUTERINTERFACE，BUTALSOSIMPLETOHANDLEOPERATE，EASYTOLEARNANDUNDERSTANDTHISSYSTEMTAKESWINDOWSASITSAPPLYSTAGESYNTHETICUSEOFTHEMECHANICSOFREASONING，PREDICTION，ANDEXPLANATIONISOFMEANINGFORSERVINGANDADVISINGTHECUSTOMERSTOREALIZETHEDECISIONOFWATERSAVINGCULTIVATIONANDADMINISTRATIONOFRICETHEFIMCTIONOFTHISSYSTEMINCLUDESPOSSESSESTHEFOLLOWINGTHREEMODULES1DECISIONMAKINGOFIRRIGATIONDURINGPONDINGPERIODTHISSYSTEMISBASEDONWATERDEPTHOFTRANSPLANTING，INFILTRATIONVOLUME，DAYS，MEANEVAPORATIONINTENSITYANDRAINFALLDURINGPONDINGPERIODTOFORECASTIRRIGATIONQUOTADURINGPONDINGPERIOD2DECISIONMAKINGOFIRRIGATIONDURINGGROWINGPERIODTHISSYSTEMISBASEDONTHEDEPTHOFROOTSYSTEM，DRYUNITWEIGHTOFSOIL，UPPERLIMITINDEXOFIRRIGATIONANDANTECEDENTSOILWATERTOFORECASTIRRIGATIONQUOTA；ITISBASEDONACTUALEVAPOTRANSPIRATIONCAPACITYEFFECTIVERAINFALLANDWATERRESERVEOFSOILTOFORECASTIRRIGATIONTIME3WATERSAVINGIRRIGATIONBENEFICIALANALYSISITINCLUDESECONOMICALBENEFICIALANALYSIS，SOCIALBENEFICIALANALYSISANDENVIRONMENTALBENEFICIALANALYSISII

簡介：南京林業(yè)大學碩士學位論文引進33種歐洲海棠品種繁殖栽培研究及景觀應用價值評價姓名王明榮申請學位級別碩士專業(yè)林業(yè)指導教師向其柏郭善基20050301THEBREEDINGANDCULTIVATINGOF33EUROPEMALUSSPECIESORCULTIVARSANDTHEIRORNAMENTALAPPRAISALWANGMINGMNGDIRECTEDBYPROFXIANGQIHAIABSTRACTTHISSTUDIESDESCRIBETHEBIOLOGYANDECOLOGYCHARACTERJSTICSOF33EUROPEMAIMSPECIESORCULFIVARSINMADUCEDBYSHANDONGPROVINCEGUANGHELANDSCAPESCINEETEEHNOLOGYCO，LTD，PROBINGTHEBREEDINGANDCULTIVATINGWAYSOFTHESEMALUSSPECIES，F(xiàn)IRSTLYUSINGAHPANALTIEHIERARCHYPROCESSTOEVALUATETHEIRORNAMENTALVALUEANDMAKINGSATISFIEDRESULTSAECRODINGTOTHEEXPERIMENTRESULTS，MOSTEUROPEMALUSSPECIESADAPTTOTHECLIMATEOFSHANDONGPROVINCEANDSHOWTHEIREXCELLENTCHATACTERISTICS，SOCANBEUSEDANDPOPULARIZEDINHUABEIAREA，SUCH硒MALUS‘RADIANFMALUS‘REDSPLENDERMALUS‘DOLGOMALUS‘FLAMEBUTTHEREAREALSOSOMESPECIESTHATEARMOTACCOMMODATEVERYWELL，THEIRAPPLMAFIONVALUESSHOULDTOBEOBSERVDMOREDEEPLY，SUCHASMALUSTSCHONOSMIMALUS‘LISCT’MA／US‘SPARKLER’，MALUS‘KELSEY’THESTUDIESALSODEMONSTRATETHATBUDGRAFTINGANDSCIONGRA_T陸AGARETHETWELIABLEBREEDINGWAYSOFEUROPEMALUSSPECIESINTRODUCEDTOSHANDONGPROVINCEAPPLYINGAHPTOESTABLISHACOMPREHENSIVEVALUINGMODESUITINGTOTHEEUROPEMALUSSPECIESAEERODINGTOTHISMODE，ALL33EUROPEMALUSSPECIESAREESTIMATEDANDDIVIDEDINTOFOURGREDESALLGRADSINCLUDINGTHETOPPESTBASEDONTHEDISTRIBUTECONDITIONOFVALUATIONRESULTSANDVISUALEXPERIENCEAHPMODEM婦GSATISFIEDRESULTSANDSETTLEASCIENTIFICTHEORYFOUNDATIONFORTHELARGESCALEAPPLYINGOFEUROPEMALUSSPECIESINLANDSCAPEGARDENOFCHINEKEYWORDSINTRODUCTION；EUROPEMALUS；AIIP

簡介：UNIVERSITYCODE10225REGISTERCODES15704DISSERTATIONFORTHEDEGREEOFMASTERISOLATL0NANDFUNCTIONALANALYSISOFTHEB尸C庀2GENEANDITSPROMOTERINCANDIDATESUPERVISORASSOCIATESUPERVISORACADEMICDEGREEAPPLIEDFORSPECIALITYDATEOF0RAIEXAMINATIONUNIVERSITYWANGSUIPROFYANGCHUANPINGPROFJIANGJINGMASTERFORESTTREEGENETICSANDBREEDINGJUNE2015NORTHEASTFORESTRYUNIVERSITYABSTRACTCUPSHAPEDCOTYLEDON2CUE2，AMEMBEROFTHEPLANTSPECIFICNACTRAJLSCRIDTIONFACTORFAMILYISREQUIREDFORSHOOTAPICALMERISTEMSAMFORNLATLONA11DSPECIFICATIONOFTHEORGANBOUNDARYINRECENTYEARS，ITHASATTRACTEDMOREANDMORE舭NTLONHO、VEVER，T11ESTUDYOFTHECUC2GENEALMOSTFOCUSEDOLLTHEMODELPLANTARABIDOPSISTHALIA玎口ANDSOⅡLEOTHERCASHCROPSSOFARITHASNOTBEENREPORTEDONWOODYPLANTSTOHELPPEOPLELE啪MOREABOUTTHEEXACTMECHANISMOFGROWTHANDDEVELOPMENTOFBETULAPENDULAANDUSETHEBPCUC2GENETEBUILDPLANTFORM，INTHISRESEARCH，WEPRELIMINARYANALYSISFUNCTLONANDEXPRESSIONPROFILESOFTHEBPCUC2GENEINBETULAPENDULAINTHISRESEARCH，F(xiàn)IRST，WECLONETHE印CUC2GENEUSINGPCRTECHNOLOGY，CONSTRUCTTHEDLANTEXPRESSIONVECTORCONTAININGTHECAMV35SSTRONGPROMOTERANDANALYZETHEGENESEQUENCENEXT，AREALTIMEPCRISUSEDTOQUANTIFYTHEZ汐CUC2GENEEXPRESSIONO士DIFFERENTTISSUESOFHALFYE廿OLDBETULAPENDUTASEEDLINGSTHEN，WECLONETHEBPCUC2GENEPROLNOTERFROMTHEGDNAOFBETULAPENDULA，CONNECTITWITHTHELUC2REPORTERGENEANDFU8EITINTOAR口BIAOPS魄THALIANAUSINGTHEPLANTIMAGINGSYSTEM，WEANALYZETHETISSUESPECIFICLTYOFTHEBPCUC2GENEEXDRESSIONATLAST，WEFUSETHEOPCUC2GENEINTOARABIDOPSISTHALIANAA11DOBSERVETHEPHENOTYPECHANGESOFTHETRANSGENICARABIDOPSISTHALIANASTRATUSWHICHHAYEHIGHLEVELEXPRESSIONOFTHEBPCUC2GENETHERESULTSINDICATETHAT1FORECASTSHOWEDTHATTHEBPCUC2GENECONTAINEDTHREEEXON8ANDTWOINTRONS，2126BPOFFULLLENGTHSEQUENCE，52BPOF5’UTR，204BPOF3’UTRAND1065BDOFCDS2THEREWASASIGNIFICANTDIFFERENCEOFTHEBPCUC2GENEEXPRESSION鋤ONGDI虢RENTTISSUESOFBETULAPENDULASEEDLINGSINDESCENDINGORDERTHEEXPRESSION1EVELOFTHEBPCUC2GENEWERESTEMTIPS，STEMS，ROOTSANDLEAVES，ANDTHEEXPRESSIONOFSTEMTLPSWASOTIMESHIGHERTHANTHATOFLEAVES3THEBPCUC2COREPROMOTERONLYCONTAINEDTATABOXANDINITIATORANDTHEWHOLEPROMOTERSEQUENCEALSOHADSOMANYKINDSOFCISACTINGELEMENTS，INCLUDINGTISSUESPECIFICEXPRESSION，PHYTOHORMONEANDENVIRONMENTALSTRESSRE8PON8E，TR肌SCRIPTIONFACTORBINDING，ETC4THEBPCUC2PROMOTERDROVETHELUC2REPORTERGENETOEXPRESSINSAM，LEAFMARGINSANDFLOWERS，OFWHICHTHEEXPRESSIONINSAMWASTHEHIGHEST5THEPHENOTYPEOFBPCUC2OVEREXPRESSINGPLANTSEXHIBITEDMULTIPLESAMS，DIVIDEDCOTYLEDONSINCREASEDROSETTELEAVESANDMAINSTEMS，SMALLERORGANS，SHRUNKENPLANTHELGHT，DELAYEDDEVELOPMENTETCTHERESUITSSUGGESTTHATTHEBPCUC2GENEMAINLYEXPRESSESINMERISTEMTHROUGHOUTTHEGROWTHCYCLEOFBETULAPENDULA，ITPLAYSANIMPORTANTROLE一II

簡介：上海水產(chǎn)大學碩士學位論文三種常見滸苔生活史的初步研究姓名王曉坤申請學位級別碩士專業(yè)海洋生物學指導教師馬家海20070501上海水產(chǎn)大學碩十學位論文PRELIMINARYSTUDYOQLIFEHISTORIESOFENTEROMORPHA／／NZAENTEROMORPHPROLIFERAANDENTEROMORPHAINTESTINALISABSTRACTTHEPRESENTSTUDYWASUNDEFTAKALINLIFEHISTORYOFENTEROMORPHAFORARTIFICIAL辯。DINGANDCULTIVATIORLONTHEONEHANDTHEPURPOSEWAST0ACCMULATETHEBASICMATEFIALSABOUTRESEARCHCONCERNINGSPECIESOFTHEMARINEGREENMACROALGAENTEROMORPHAWHICHWASSCARCELYSTUDIEDANDITSMASSCULTIVATIONHADNEVERBEENMAKEUPDATEINALILLA’ONTHEOTLI￡RH鋤D，曲RESEARCHPRODDEDTHEKISICTHEORIESFORTHEEFFECTIVECULTIVATIONM曲ODSANDTECHNIQUESOFENTEMMORPHAINORDERTOMAKEITBECOMEANEWSPECIESOFMARINEGREENMACROALGAECULTIVATIONINCHINATHECONVENTIONALSYSI砌CIDENTIFICATIONOFENTEROMORPHALINZ珥ENTEROMORPHAPROLIFERAANDENLEMMORPHAINTESTINALISW腫COM刪INCHARACTERIZATIONLIFEHISTORYDISTRIBUTIONECOLOGYANDCELLWAITANDWORKOUTKEYOFTHATTHREESPECIESINTHEPRESENTSTUDYTHE鋤JTHOFOBSERVEDAND砌PORTEDTHEMORPHOLOGICALCLI出DCLEISANDDEVELOPMENTPROCESSINALLSTAGESOFTHELIFEHIS呻OFE1IMAEPROLIFERAANDEINTESTINALIS婦OUGHCROSSINGEXPERIMENTSANDU11IALGALCULTURESFORGENERATIONSFORTHEFIRSTTHNEINCHINATHEALLTHORHADNEWDISCOVERIESANDCOMPLEMENTSABOUTTHELIFEHISTORYOFEMEROMORPHABIFLAGELLATEGANLETESAREPRODUCEDBYDIOECIOUSMEMBRANOUSGAMETOPHYTESANDTHENTHEYJOINTOGETHERANDGROWINTOASPOROPHYTEBOTHTHEMALEANDTHEFEMALEGMNETESOFEMEMMO伊HACANGENNINATEPARTHENOGENETICALLYANDDEVELOPINTONGAVTHALLUSTHESPOROPHYTETHENILNL婦GOESMEIOSIS，PRODUCINGQUADRIFLAGELLATEZOOSPORESANDEACHSETTLEDZOOSLOREGROWSINTOAGAMETOPBYTETHE珥，0RNI‘ISOFENTEROMORPHAHASTWOAPPROACHESONEISTHEGAMETEDIFFUSEDFROMGAMETANGIAOFGAMETOPHYTESSUCCEEDSINDEVELOPINGINTONEWTHALLUSTHEOTHERISTHETILLDIFFUSEDGAMETEDEVELOPINTLE、VT11ALH略DIRECTLYLIFETLISTOIYTYPICALLYANULTEINAFIONOFISOMORPLIC，UNISEXUALHAPLOIDGAMETOPHYTESANDDIPLOIDSPOMPHYTESSOMEPOPULATIONSOFENTEROMORPHALIMAREPRODUCINGSTRICTLYASEXUALLYBYM最LNSOFEITHERBIFLAGEUATEDNEUTRALSWAMIERSORQUADRIFLAGELLAXEDSWARMERSEACHSPECIESPRODUCESALL唧班UNISEDATEFILAMENTCLERNLINGSLATERBL∞OMEPLURISERIATEBYREPEATEDONGI刪CELLDIVISIONSPERPENDICULARTOTHE飄肛Ⅱ

簡介：ABSTRACTABSTRACTINMISSTLLDY，WOODDESTROY如NGICD，2F印向DMP“，P口玎口WASUSEDTOTSCOTSPINEHEARTWOODSFROMT、VOTRIALSOFPROGENYTESTTHEAIMWASTOSELECTTHEDECAYRESISTANTANDSUSCEPTIBLEPOPULATIONAILDF撇ILYFORMESPECIESONT11EBASISOFTHIS，THERELATIONSHIPBETWEENCONCENTRATIONSOFSTILBENESINTHES鋤PLESANDTHEHEARTWOODRESISTANTABILIT，WASSTUDIEDACCORDINGLYT11ECONCENTRATIONSOFSTILBENESCALLBEUSEDTOSELECTTHEDECAYRESISTANTWOODSTLLJCTUREWOODANDTHEDECAYSUSCEPTIBLEWOODPULPWOOD1THERESULTSHOWEDTHATTHEDENSITYLOSSESOFTHES鋤PLES行OMTHETWOTRIALSWEREDI虢RENTALONG謝THTHEDI行ERENTROTTIMEOFTHEHEANW00DFUNHENILORE，THEDI虢RENCESBETWEEN‰ILIESANDINDIVIDUALSWEREALSOSIGNIFICANTINTHEWOODDENSITYLOSSESNISRESULTCOULDPROVIDEUSE如LINFLOMATIONFORSELECTIONOFDECAYRESISTANTA11DSUSCEPTIBLECOLONIES210DECAYRESISTANTTREESAND10SUSCEPTIBLETREESWERERESPECTIVELYSELECTEDTODOTHECHEMICALA11ALYSISTHEMEANDENSITYLOSSOFTHESELECTEDDECAYRESISTANTPOPULATIONWAS69MG／CM3TRIAL1AND5MG／CM3TRIAL2，AND也ATOFMESELECTEDDECAYSUSCEPTIBLEPOPULATIONWAS231MG／CM3TRIAL1AND169M∥CM3TRIAL23BYMEANOFFOLINCIOCALTEUTECLULOQUE，USINGACETONEASS協(xié)DARD，TOTALPHENOLCONTENTINTHES鋤PLES、ⅣASMEASUREDTHERESULTSHOWEDTHATTHEDI虢RENCEBETWEENTHEDECAYRESIST觚TA11DSUSCEPTIBLEPOPULATIONW2LSSTATISTICALLYSIGNIFICALLTINBOTLLTRIALS4INTHECHEMICALAILALYSIS，THREEBNDSOFSTILBENES、ⅣEREFOUNDINTHESAMPLESBYUSINGGLCMSHP6890THEYWEREPINOSYLVINPS，PINOSYLVINMONOMETHYLETLLERPSMANDPINOSYLVINDIMETLLYLETLLERPSDPSDWASONLYFOUNDINPANOFMESAMPLESWHEREWASLOWINCONCENTRATIONTLLEMEAJLCONCENTRATIONOFTOTALSTILBENESINDECAYRESISTANTPOPULATIONWAS743M∥GTRIAL1AND51OMG／GTRIAL2；ANDMATINDECAYSUSCEPTIBLEPOPULATIONWAS630M∥GTRIAL1AND477MG儋TRIAL2THEVARIATIONOFTOTALSTILBENESCONTENTBETWEENTWOPOPULATIONSWASALSOSIGNMCANTTHERESULTSIMPLIEDTHATSTILBENESCONCENTRATIONINTHES鋤PLESMI曲T斫RECTTOT11EWOODDECAYTOLERAJLCE，BUTITWASNOTTHEONLYREASONTOEXPLAIN也EVARIATIONINWOODDECAYAMONGT11ES鋤PLESFUNHENLLORE，AHIGHCONCENTRATIONOFPHEN01ICSWASCORMECTEDTO也ELOWHY伊OSCOPICI夠OFTHEWOODKEYWORDSSCOTSPINE，BRO、VNROTMNGUS，DECAYRESISTANCE，PHENOLICS

簡介：分類號S59密敏單位代碼10389學號S1030805福建農(nóng)林大學碩士學位論文氟苯尼考在歐洲鰻鱺體內(nèi)的藥物代謝動力學研究學科門類農(nóng)學一級學科獸醫(yī)學二級學科臨床獸醫(yī)學研究方向動物疾病防治研究生陳星星指導教師王壽昆教授樊海平研究員論文完成時間二00六年四月福建農(nóng)林大學碩上論文氟苯尼考在歐洲鰻鱺體內(nèi)的藥物代輯F動山學1“鉦CMAX為2103712“GM】～，CI／F為0330051，H～，IJC。一為306465288MGL1H1。注射給藥血液中主要的藥物代謝動力學參數(shù)如下KA為014005H一，T。。為583240H，TMAX為356249H，CMAX為4652245LUGML，CL／F為0，10003L一，AUC∽。為8753920818MG∥H一。結果表明，口服氟苯尼考后，鰻鱺吸收迅速、分布廣泛、抗菌活性好、體內(nèi)的半衰期較長，殘留小，臨床上可推廣使用。根據(jù)消除規(guī)律的分析，I臨床給藥初次劑量40MGKG～，維持劑量20MGKG一，每同一次。通過休藥期軟件WTI4計算，休藥期應不少于6D。關鍵詞氟苯尼考歐洲鰻鱺藥物代謝動力學2

簡介：V903698分類號6623授予學位單位代碼10434編號Z2002122山柬蓑爹大學碩士專業(yè)學位論文歐洲李生物學特性及栽培技術研究STUDIEDOFBIOLOGICALCHARACTERISTICANDCULTIVATINGTECHNOLOGYFOREUROPEANPLUM姓名專業(yè)類別研究方向?qū)W院拳闖農(nóng)業(yè)推廣果樹學園藝科學與工程學院指導教師彭福田副教授劉世琦教授201005年12月10日Y30360B關于學位論文原創(chuàng)性和使用授權的聲明本人所呈交的學位論文，是在導師指導下，獨立進行科學研究所取得的成果。對在論文研究期間給予指導、幫助和做出重要貢獻的個人或集體，均在文中明確說明。本聲明的法律責任由本人承擔。本人完全了解山東農(nóng)業(yè)大學有關保留和使用學位論文的規(guī)定，同意學校保留和按要求向國家有關部門或機構送交論文紙質(zhì)本和電子版，允許論文被查閱和借閱。本人授權山東農(nóng)業(yè)大學可以將本學位論文的全部或部分內(nèi)容編入有關數(shù)據(jù)庫進行檢索，可以采用影印、縮印或其他復制手段保存論文和匯編本學位論文。保密論文在解密后應遵守此規(guī)定。論文作者簽名導師簽名垃日期蘭三生

簡介：中國和歐洲小麥品種資源WX基因的遺傳多樣性研究摘要WAXY蛋白也稱顆粒結合淀粉合成酶GBSS，基因位點命名為WX，其表達與否直接制約著直鏈淀粉的合成小麥品種的直鏈淀粉含量與WAXY蛋白含量高度正相關，尤其是WXBL亞基缺失類型小麥品種具有較低直鏈淀粉含量，較高的支鏈淀粉含量對面條品質(zhì)有很強的正向效應。因此，小麥膿基因的研究對淀粉性狀及面條品質(zhì)改良具有重要意義。本研究用2對STS標記、1對SSR標記和1對基因特異分子標記，對1739份中國小麥地方品種WX基因的3個位點進行了鑒定，共篩選出31份突變體。其中，WXAI突變體3份，WXBL突變體25份，更重要的是發(fā)現(xiàn)了3份地方品種WXDI突變體并用SDSPAGE方法對部分材料的WX基因表達進行了功能驗證。另外，利用相同分子標記對來自歐洲18個國家共384份材料進行了WX基因突變類型檢測，僅檢測到WXBI種突變類型共19份，未發(fā)現(xiàn)WXALWXD1突變體。并對中國和歐洲材料突變類型及頻率進行了比較分析。整體看，我國地方品種較歐洲材料具有更豐富的WX基因突變類型。選用綜合性狀較好的WX基因突變材料，如紅芒麥、竹稈青、和尚頭、灰三糙等配制了10個雜交組合，在幾代檢測出4株WXAL和WXB1的雙隱性純合體，并與鄭麥9023、鄭麥9405進行了雜交。因WXD1位點的基因特異標記是顯性標記，不能有效檢測純合性，只檢測到4株WXAL純合WXDL可能雜合的植株，3株WXBI純合WXD1可能雜合的植株，這些材料自交后便可獲得雙隱性純合體。關鍵詞小麥WXAL服B1WXDI分子標記圖表目錄圖11直鏈淀粉結構””““，””””，““””“””““““““““’“，，二”1圖12支鏈淀粉結構二”””“”””””’”二”””二，”，”“”，一””一”1圖1一普通淀粉比例組成二，’，“，’”””“，”“二，’””””“”“”一”2圖14淀粉粒的形成二?！薄薄薄薄薄薄薄薄啊薄薄倍?圖1一六倍體小麥WX易發(fā)生突變區(qū)段示意圖二””””，““”““”“““”““””’““一N圖31WXAL位點STS標記的鑒定，，，，，，“。，，23圖32WXBL基因STS標記的檢測24圖33WXBI基因STS標記的檢測““““““”“””’“““““““”一”25圖34部分突變材料的蛋白檢測“““““””“””””二”28圖35突變材料的BAFBAR引物對檢測二，””“””“’““”“，二“29圖36突變材料的VTLFVTR引物對，”“““二””””””二“““““”””一”“一”””””二“30圖41中國和歐洲小麥品種WX基因突變比較35圖5一和尚頭與紅芒麥的F2群體的PCR分析AWXAL位點，BWXBI位點37表11六倍體小麥3個WX基因之間同源性、相似性和非同義轉(zhuǎn)換同義轉(zhuǎn)換，10表12糯質(zhì)小麥類型及其直鏈淀粉含量16表21SDSPAGE凝膠配方“““二“’“?！啊耙弧薄薄啊啊倍?2表31不同麥區(qū)WXAIWXBL和WXD1基因突變類型二“””””，““““”“，””““絲表32WX基因突變材料詳細清單?！薄薄薄薄埃薄啊薄薄薄薄啊薄薄薄薄薄啊?，”““”””””“絲表41新引進材料在歐洲的分布““”“““”““”“二，”““””“”，一”32表42不同國家小麥品種的WXALWXBI和WXDI突變類型33表43歐洲WX基因突變材料名單””“，””””?！耙弧币弧?4表51雜交所用突變材料名單3BIV

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簡介：中山大學博士學位論文斜帶石斑魚早期生活史及種苗繁育技術研究姓名張海發(fā)申請學位級別博士專業(yè)海洋生物學指導教師林浩然20050609卵所含的蛋白和脂肪量自然就高于秋季產(chǎn)的卵。在育苗實踐中，要充分利用春季產(chǎn)卵質(zhì)量好的優(yōu)勢，大批量開展育苗生產(chǎn)。詳細描述從受精卵到初孵仔魚的28個具體發(fā)育時期的形態(tài)特征和發(fā)育時間，將斜帶石斑魚胚胎發(fā)育劃分為卵裂期、囊胚期、原腸胚期、神經(jīng)胚期和器官形成期。在水溫2505℃、鹽度310、28H30MIN完成整個胚胎發(fā)育孵化出膜。PH78的海水中，斜帶石斑魚胚胎歷時胚后發(fā)育主要根據(jù)卵黃囊、腹鰭棘及第二背鰭棘、鱗片、體色的變化分為仔魚期、稚魚期、幼魚期。仔魚期根據(jù)卵黃囊的有無又分為早期仔魚和晚期仔魚。在水溫245292℃，海水鹽度288335，PH7585的海水中，培育至36D，發(fā)育最快的斜帶石斑魚結束仔魚期進入稚魚期；培育至42D，發(fā)育最快的稚魚完成變態(tài)，成為幼魚。斜帶石斑魚胚后發(fā)育過程中最明顯的變化是腹鰭棘與第二背鰭棘以及鰭棘上小刺的長出與收回。在培育水溫2505。C、鹽度3L一32條件下，斜帶石斑魚仔魚孵化后第3D開口并進入攝食期，孵化后55D，不能建立外源性營養(yǎng)的仔魚分別進入不可逆點PNR期，幾乎與此同時，卵黃囊和油球耗盡，仔魚混合營養(yǎng)期為2～3D，從初次攝食到進入PNR期的時間為25D仔魚在不同饑餓階段初次攝食發(fā)生率的變化式型是開始較低，此后逐步上升，高峰期出現(xiàn)在卵黃囊接近耗盡時，此后迅速下降記錄到的最高初次攝食發(fā)生率為767％；饑餓仔魚的死亡高峰出現(xiàn)在孵化后46D，至第8D，饑餓仔魚全部死亡卵黃囊期仔魚的生長可以分為3個期相初孵時的快速生長期，卵黃囊消失前后的慢速生長期，以及在不能建立外源性攝食后的負生長期。在仔魚培育階段每天定時對仔魚的攝食作跟蹤調(diào)查，以了解仔魚對輪蟲、豐年蟲、蒙古裸腹涵的攝食強度和攝食發(fā)生率的變化。結果表明，輪蟲和蒙古裸腹潘是斜帶石斑魚仔魚培育階段非常重要的餌料種類，前者在仔魚培育前中期占有極其重要的作用，后者在仔魚培育中后期占有極其重要的作用；豐年蟲由于存在營養(yǎng)缺陷而屬于仔魚培育階段一種爭議較大的餌料，只能輔助使用育苗實踐中，在H18之前保證輪蟲的充足供應以及在H1927期間維持輪蟲的適量供應是必須的，否則很可能導致育苗的失敗在H1518期間保證蒙古裸腹潘的適量供應以及在H1926期間維持蒙古裸腹潘的充足供應是非常重要的，或者有其它大型枝角類、橈足類取代蒙古裸腹潘也是可以的。II

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簡介：Y81687F害踢震案大垮碩士學位論文幾個歐洲生態(tài)型杏物質(zhì)代謝與花芽分化相關性的研究研究生指導教師專業(yè)名稱研究方向所在學院邱學思劉國成研究員果樹學果樹栽培生理園藝學院2006年4月沈陽農(nóng)業(yè)大學砸士學位論史摘要杏樹作為重要的核果類果樹之一，栽培上普遍存在花器官發(fā)育不良的現(xiàn)象，從而成為制約杏樹豐產(chǎn)的主要原因。本研究以黃金杏、凱特杏、金太陽杏三個歐洲生態(tài)型杏為試材，從花芽分化的發(fā)育生理學入手，分別從解剖學，營養(yǎng)生理學，激素生理學角度，對花器官分化、孕育過程進行了較為系統(tǒng)的研究，試圖探明杏花芽分化過程及其相應的響應規(guī)律。試驗結果摘要如下1三個歐洲杏品種花芽分化過程中無雄蕊敗育現(xiàn)象，整個發(fā)育過程良好。2三個歐洲杏品種均存在雌蕊敗育現(xiàn)象，從雌蕊原基形成一直到八核胚囊分化完成，形成成熟雌配子體，這其中的每一個步驟都可能出現(xiàn)雌蕊敗育的不良情況。3杏花芽分化的生理分化期，葉片同化生成的碳水化合物集中供給花芽。無論在葉片還是在短果枝中可溶性糖含量都偏低，葉片中淀粉含量較高，枝條中較低，此時的淀粉酶活性不強，表明在葉片中光合同化生成的產(chǎn)物從葉片輸出，直接供給花芽分化使用，為其提供合成氨基酸、蛋白質(zhì)等必需物質(zhì)的碳架元素，而少量從枝條輸出4杏形態(tài)分化過程中，各分化時期所需養(yǎng)分含量存在差異。雄蕊原基分化期，可溶性糖和淀粉含量較高；雌蕊原基分化期，碳水化合物以淀粉為主。5全氮含量與杏花芽分化相關性不顯著?；ㄑ糠只谌~片全N平均含量黃金杏金太陽杏凱特杏。6杏花芽生理分化期向形態(tài)分化期的轉(zhuǎn)變時期分化臨界期，氨基酸需求量雖大。此時期需求量比其它時期高很多，結合可溶性糖在此時期含量處于中上等水平，推斷出花芽形態(tài)的建成需要大量的氨基酸以合成相應的蛋白質(zhì)，并且其需要量高于生理分化期。7花芽孕育即生理分化過程款間，C烈比值出現(xiàn)一個小高峰，在雄蕊原基分化期，再次出現(xiàn)峰值，說明了該比值對花芽分化，特別是雄蕊原基分化有重要影響。8葉片與枝條中P、K含量動態(tài)變化呈現(xiàn)相反的趨勢，枝條中磷含量呈現(xiàn)“低高一低”的變化動態(tài)，與葉片中“高一低高”的趨勢相反，可能表明枝條作為重要的貯藏和運輸通道，葉片和根系中合成或吸收的碳水化合物及礦質(zhì)元素在枝條中起到一種緩沖作用，在兩個“源”輸出不足時，枝條能夠起到暫時的補給功能。9生理分化期，內(nèi)源激素需要高水平的IAA、DHZR／IAA比。形態(tài)分化期ABA含量維持低水平。IAA含量升高對杏花芽雄性器官分化有利。IAA／ABA比控制在O284左右有利于黃金杏花芽誘導，而在杏花雄蕊原基分化過程中需要較

簡介：中國野生葡萄新基因轉(zhuǎn)化歐洲葡萄品種的研究西北農(nóng)林科技大學，陜西楊凌，712100碩士研究生孫偉生指導教師王躍進教授博士合作指導教師張劍俠副教授摘要無核葡萄是當今世界葡萄消費和育種的重要方向之一，但生產(chǎn)上主要栽培的歐洲葡萄無核品種抗病性差，中國野生葡萄是抗葡萄真菌病害的重要基因資源。通過向歐洲葡萄無核品種轉(zhuǎn)移抗病基因是培育抗病無核品種的有效途徑。利用課題組通過MRNA差顯技術從中國野生葡萄高抗白粉病的華東葡萄白河一35一1中克隆的與抗白粉病相關的基因乙二醛氧化酶新基因CDNA全長序列，通過構建表達載體PWR306一GLOXRG，采用農(nóng)桿菌介導法對歐洲無核葡萄品種無核白、底來特和愛莫無核及煙草，進行了轉(zhuǎn)基因研究。取得的主要結果1無核葡萄試管苗的建立對經(jīng)4℃低溫沙藏的葡萄硬枝，冬季以10的石灰氮涂芽處理，促進萌芽，用新梢建立葡萄無菌系。對葡萄新梢剪取單芽莖斷進行擴繁適宜的繼代培養(yǎng)基為NN6901M叭IBA，生根培養(yǎng)基為12MS0LM叭NAA。2葡萄器官再生途徑的建立以無核葡萄葉片、葉柄及莖斷為外植體，進行再生研究。篩選獲得的適宜培養(yǎng)基分別是，葉片再生MS2OMGLTDZ0LMGLNAASOOMGLCHSGLPVP葉柄和莖斷再生NN694OMGLTDZ0LMGLNAASOOMGLCHSGLPVP。3葡萄胚狀體途徑方式的再生以愛莫無核自交的胚珠為試材，剝胚進行胚狀體誘導。幼胚在培養(yǎng)基WP固0225M妙6一BA十60留L蔗糖2留L活性炭5OM留L水解酪蛋白上胚性愈傷和胚狀體的誘導率為75在培養(yǎng)基WP固LOMGLTOZ60歲L蔗糖ZGL活性炭SOOMGL水解酪蛋白上胚性愈傷和胚狀體增殖良好。4中國野生葡萄屬乙二醛氧化酶植物表達載體的構建雙酶切重組質(zhì)粒PGEM一TGLOX飛與中間表達載體PWR306，將GLOXRG基因片段與線性表達載體PWR306進行定向連接，構建了茂合成酶基因的植物表達載體。通過酶切、電泳鑒定，證明GLOXRG基因己成功構建到植物表達質(zhì)粒PWR306中，將此克隆命名為GENETICTRANSFMATIONOFTHCNOVELGENE1NVOLVEDINGLOXALOXIDASEEFROMTHECHINESCWILD片公夕““而理吮“紐TAINTOEUROPCANGRAPCVARITY扭。曲WESTAFUN城招ITY，YAUGLING，SHAA盯1，71210，CHINAPOSTGRADUATESUN、甲七如BENGSUPERVISORPROFW幽NGYUEJINCOSUPE口ISORVICEPROFZHANGJIAUXIAABSTRACTSEEDLESSGRAPEISONEOFIMPO比知TDIRECTIONOFCONSUMPTIONANDBREEDINGHOWEVER，THEMOSTDOMINATINGCDTIVARSARE矛公力VIH毋RAANDSENSITIVETODISEA巧ETHECHINESE誡LD子份T臺AREIMPORTANTGENERESOURCESWHICHAREHIGHLYRESISTANTTOFUNGALDISEASEOFGRAPESITISANE月乞CTIVEAPROACHTOBREEDTHERESISTANCECULTIVARSOFSEED1ESSGRAPEBY之“的SFE幣NGRESISTANTGENEINIOTHECULTIVARSOF巧TISV動訴RATHEFUILLENGTHSEQUENCEOFNOVELGLYOXALOXIDASERELATEDGENEGLOXIGFROMTHECHINESEWILD玲ISPSE“DETICUIQTABAIHE一35一LWASCLONEDBYMRNARACEINOURRESEARCHTEAMHI面SSTUDY，WECONSTRUCTEDTHEPLANTEXPRESSIONVECTOROFGLOXRG，明DTHENWEINTRODUCEDTHEAIMGENEINTOTHESEED1ESSCUITIVARSOF不公15VIH滬RATHOMPSONSEEDLESS，DELIGHTSEEDLESSANDOMLOSEEDLESSANDTHEMODELPLANTTOBACCOTLLERESULTS認℃REASFOLOW1OB面NEDTHEGERMFREETUBLESEDINGOFTHESEEDLESSGRAPETHEGRAPEVINEDORMANCYBRANCHESWEREBURYINGINTHE從℃TSANDUNDERLOWTEMPERATURE，4℃THENTHEBUDSWERESMEAREDWITHTHELO?LCIUMCYANAMIDE，INSERTEDANDGERMINATEDTHESTERILES”之EMWASSET叩雨THTHEGEN刀INATINGSHOOTSTHECOLO叮WASPROLIFERATEDWITHSIMPLEBUDAFTERTHEFIRSTCULTURETHEFITINGSUBCULTUREMEDIUMWASNN690LMGLLIBAANDTHE方TINGROTINGMEDIUMWASL2MS0LMG幾NAA2THEREGENERATIONSYSTEMOFOTGANSWERESETUPTHEGRAPE，SLEAVES，PETIOLESANDS把M認℃RET介口SFEREDTHEDIFEREN之REGENERATIONCULTURE即DRESEARCHEDTHEREGENERATIONTHESELECTEDRESULTSINDICATEDTHATTHEFITINGREGENERATIONMEDIUMOFLEAFWASMS2OM妙TDZ0LMGLNAASOOMGLCH5叭PVPANDTNEFITINGREGENERATIONMEDIUMOFPETIOLEANDSTEMWASNN6二4OMGLTOZ0LMGLNAA500M留LCH59幾PVP3REGENERATIONOFTHEEMB仃OID

簡介：東北林業(yè)大學碩士學位論文轉(zhuǎn)基因歐洲白樺降解及目的基因水平轉(zhuǎn)移生態(tài)風險研究姓名劉欣欣申請學位級別碩士專業(yè)遺傳學指導教師王秋玉TUIJAARONEN20060601東北林業(yè)大學碩士學位論文ABSTRACTINTHISSTUDY，THELEAVESOFTRANSGENICSILVCRBIRCHAREUSEDASTHEMATERIALS，WHATISUSEDTOANALYZEMEEF絕CTOFDECOMPOSITIONBYCHANGINGTHELIGNINCOMPONENTOFTRANSGENICPLANTS，ANDTHEPOSSIBILITYOFTRALLSGENEHORIZONTALTRALLSFELTHEAIMISTOACCESSTHEECOLO衛(wèi)ICALRISKOFTRANSGENICMATERIAL’SUTILIZATIONANDTOPROVIDEUSE如LINFBMLATIONTOBOTLLOFTHEPRODUCTIONAILD}APPLICATIONOFTRANSGENICBIRCHINTHEFUTURC．THETRANSGENICBIRCHCLONESINFOURYEARSOLDWITHTWOGENOTYPES35S．PTCOMTANDUBBLPTCOMTARESELECTED，THELEAVESARECOLLECTEDFORDECOMPOSITIONINTWOSOILTYPES，ONEISFORCSTSO“丘OMBIRCHPLAJLTATION，THEOTHERISFHRNLFIELDSOIL．THEMASSLOSSISCALCULATEDBVMEASUFINGTHEWEIGHTSOFLEAVESBEFOREANDARERDECOMPOSITION．THERESULTSSHOWT“若ITHECHAILGEOF1IGNINCOMPONENTHASAFRECTEDONDECOMPOSITIONOFTHEMATERIALS．ONECLONEN舯EDNO．23，WHOSES／GREDUCESBY69％TO72％，HASHIGHERMASS10SSBY7_2％A11D1．8％INFA唧SOILANDFORCSTSOILRESPECTIVELY。AJSO，T量LEHO“ZONTAITRANSFERPOSSIBILITYOFTARGETGENEFROMTR趾SGENICBIRCHISDISCUSSEDINTHISSTUDYTHE鋤PICIHINANDKANAMYCINRESISTANCEOFTHESOILMICROBESWHOTOUCHANDDONOTTOUCH仃ANSGENICMATERIALSISTESTED，THERESULTSSHOWSTHATBOTHANTIBIOTICRESISTANCEAREINCREASED，INWHICHANLPICILIINRESISTAILCEINCREASES1．42％，W恤LEKALLAMYCINRESISTANCEINCREASESO．7％INTHEFA肌SOIL，A11D鋤PICILLINRESISTATLCEINCREASES5．64％，WHILEKAILAMYCINRCSISTAJLCEINCREASES7．55％JNTHEFORES￡SOJL．INTHISCASE，J￡PROBAB】YHASSOMESKSDF仃ANSGENEHORIZONTALTRANSFERTOSOILMICROBES．GENERALLYSPEAKING，THCCHAJLGESOFANTIBIOTICRCSISTARLCEINSOILMICMBESAREAFRECTEDBYMANYFACTORS，SOITISSTILLDIMCULTTOPROVEWHETHERORNOTTHETARGETGENEOFTHEMATERIALSISCLEARLYTRANSFERREDINTOSOILMICROBESATPRESENT．ACCORDINGTOTHISSTUDY，THETRANSGENICSILVERBIRCHTHATTHELIGNINCOMPONENTHASBEENCHANGEDDOESPOSSIBLYA腩CTTHEDECOMPOSITIONOFTHEMAFERIALS．SINCETHEEXPRESSIONLEVEIOFTHETA唱ETGENEANDITSTMNSFERARECOMMLLEDBYMANYFACTORS，THEFHRTHERSTUDYONMEECOLOGICALRJSKOFTRANSGENICCOMTBIRCHWILLBESTILLNEEDED．1EYWORDSSILVERBIRCH，TRANSGENE，DECOMPOSITION，ECOLOGICALRISK