胞漿型磷脂酶A-,2-α（cPLA-,2-α）在小鼠早期妊娠子宮中的表達(dá)與調(diào)節(jié).pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文胞漿型磷脂酶Aα（cPLAα）在小鼠早期妊娠子宮中的表達(dá)與調(diào)節(jié)姓名：于浩申請學(xué)位級別：碩士專業(yè)：發(fā)育生物學(xué)指導(dǎo)教師：楊增明20040601ExpressionandRegulationofCytosolicPhospholipaseA2aGeneinMouseUterusduringEarlyPregnancyAbstractCytosolicphospholipaseA2a(ePLA2呻，oneofthepho

2、spholipaseA2superfamily(PLA2)membersisubiquitouslyandCOnSfitutiveiyexpressedinmostcellsandtissuesn∞activationofcPLA2ⅡisregulatedbyCa壙concentrationandphosphorylation，cPLA2ucatalyzesthehydrolysisofthesn2positionofmembraneg

3、lycarophospholipidstoliberatearachidonicacid(AA)andisamajorproviderofarachidonicacidforthecyclooxygenase(cox)systemforthebiosynthesisofpmstaglandins(PGs)PGssynthesizedbythispathwayareimplicatedinvariousfemalereproductive

4、functionswhichisundertheregulationofcPLA2aTheaimofthisstudywastoexaminecPLA2agoneexpressionandregulationinmouseuterusduringearlypregnancypseudopregnancydelayedimplantationartificialdecidualizationandhormonaItreatmentbyin

5、situhybndizationandimmunohistochemistrycPLA，旺immunostainingwasdetectedintheiuminalepitheliumandglandularepitheliumofmouseuterusfromdaysl々O5ofpregnancycPLA2arnILNAsignalcouldonlybedetectedfromdays2to4ofpregnancy，Astronglc

6、velofcPLAzaexpressionwasobservedintheepitheliumonday3ofpregnancycPLAzumRNAandimmunostainingwerelocaledindecidualizedcellsinthemesometriumsidefromdays6to8ofpregnancyUnderartificialdeciduaIizationcPLA20mRNAsignalandimmm、os

7、tainingweremainlyseeninthedecidualizedcellsatthemesometriumsidecPLA2uimmunostainingwasobservediriluminalandglandularepitheliumofmouseutenlsOildays1。4，5and8ofpseudopregnancycPLA2amRNAsignalcouldonlybedetectedon4，5and8ofps

8、eudopregnancyIntheutemsunderdelayedimplantation，cPLA2umRNAandimmunostainingwerestronglylocatedintheluminalandglandularepitheliumcPLA2uproteinexpressionwasweakenedandcPLA2amRNAsignalcouldhardlybedetecteda燭ractivationInthe

9、ovariectomizedmicecPLA，atuRNAandimmunostainingweredetectedintheluminalandglandularepitheliumcPLA2aimmunostainingremainedunchangedbyhormonaltreatmentEstrogendownregulatedcPLA2umRNAsignalandprogesteronehadnoef耗ctsoncPLA2am

10、RNAsignalInconclusion，ourresultssuggestthatcPLAzamaybecloselyrelatedtoembuoimplantationanddecidualizationPestgradnate：YuHaoSpeciality：DevelopmentalBiologySupervisor：ProfessorYangZengmingKeywords：cPLA2旺’mouse，uterus，impla