紅酵母中活性成分的研究——類胡蘿卜素的工藝優(yōu)化及紅酵母細(xì)胞壁的應(yīng)用.pdf

1、湖北工業(yè)大學(xué)碩士學(xué)位論文紅酵母中活性成分的研究——類胡蘿卜素的工藝優(yōu)化及紅酵母細(xì)胞壁的應(yīng)用姓名：羅璇申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：發(fā)酵工程指導(dǎo)教師：王金華20080501湖北工業(yè)大學(xué)碩士學(xué)位論文AbstractTherearelotsofactivecompositionsinRhodotorulaspForexamplecarotenoidscouldbeusedasfoodadditive，medicalantioxidant，feeda

2、dditiveOntheotherhand，theRhodotorulacellwallcouldbeusedasgreenimmunefeedadditiveforitsimmuneactivationConsideringtheincreasingdemandoffunctionalf00dfeedadditiveinchinaandinnocuity，cheapnesscharactersofRhodotorulacellwall

3、，productionofcarotenoidsandapliicationofRhodotorulacellwallasfeedadditivewerestudiedThefermentationmediumproducingcarotenoidsbv融odotorulaspDwasoptimizedbyresponsesurfacemethodologyInPlackett—Burmandesign，yeastextract，(NH

4、4)2904，sucrosewerefoundtobetheimportantfactorsaffectingcarotenoidsproductionThecentralcompositedesignandRSMwereappliedtodeterminetheoptimalconcentrationofeachsignificantvariableTheoptimalfermentationmediumdescribedasfoll

5、ows：yeastextract4173g／L，(NH4)2S041360∥L，sucrose6053g／L，MgS04030g化K2HP04010g／L，lactoflavinO004g／LT11emaximumproductionofcarotenoids，1539mg／L，wasachievedusingoptimummedium，whichwas5865％higherthanusingtheoriginalmediumExtra

6、ctionofcarotenoidsfromRhodotorulaspDwasinvestigatedAcidhotisthebestmethodforbreakingeellwallTheoptimalextractionconditionofcarotenoidswasobtainedbysinglefactorexperiment，fractionalfactorialdesignexperiment，steepestascent

7、experimentandthecentralcompositedesignTheoptimalconditiondescribedasfollows：theconcentrationofextractingsolventswas4266％(VⅣ)，theratioofacetonetodimethylsulfoxidewas6247％(VⅣ)，theextractingtemperaturewas20℃andtheextracting

8、timewas90minUndertheoptimizedextractioncondition，thecarotenoidsextractedfromRhodotorulaspDwas3125％higherthantheresultbeforeoptimizationRhodotorulacellwallwasusedasfeedingadditivetobroilerchickstovalidateandevaluateitsfun

9、ctionTheresultsshowedthatRhodotoruIacellwallwasagoodimmunosuppressorThedayincreasedweight，transformrate，dyingrate，relativeweightofimmuneapparatusandthecontentofbacteriolysininbloodserumin21dayand42dayallsignificantlyincr

10、eased2000mg／kgwasthesuitablefeedingamountProductionofcarotenoidsandcellwallactivecompositionsbyRhodotorulaspDatdifferentgrowthconditionswasstudiedTherewasadirectrelationbetweentheamountof13—1，3Dglucan，mannaninthecellwall

11、andtheyieldofcarotenoidsThisresultmaybebenefittofurtherinvestigationonsyntheticpathwayofcarotenoidsandtwocellwallpolysaccharides，andalsomaybehelpfulinfurtherapplicationofcarotenoidsKeywords：Rhodotorula，carotenoids，cellwa