林蛙油小分子肽的制備及其分子量分布研究.pdf

1、吉林農(nóng)業(yè)大學(xué)碩士學(xué)位論文林蛙油小分子肽的制備及其分子量分布研究姓名：孫建忠申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：食品科學(xué)指導(dǎo)教師：胡耀輝20080601PreparationofRanaoviductmicromoleculepeptideandResearchforitsmolecularweightdistributionAbstractInthisstudyRanaoviductwasthesubstrate，caroidalkalinepro

2、teinase，extranase，pepsaseandcompproteasewereemployedtohydrolyzesubstrate，theeffectofenzymolysisWaSdeterminedbydeterminingenzymeactivitysubstrate’Sdegreeofhydrolysisandcontentofacidsolublepeptide、freeaminoacidsinthedigest

3、TheresultWas“theenzymeactivityofcaroidandalkalineproteinasewerehigherthantheotherthreeenzymes，510U／mg350U／mgrespectively；degreeofhydrolysiswashigher，123％and118％respectively；thecontentofacidsolublepeptidecontentWashigherr

4、elatively437％and503％respectively；Thecontentoffreeaminoacidswaslowerrelatively042mg／mLand056％respectivelyInsummarycaroidandalkalineproteinasewereemplyedtoprepareactivepeptideRanaoviductWashydrolyzedbycombinationoftwoenzym

5、esFirstlysubstrateWashydrolyzedbyemployingcaroid，theeffecttodegreeofhydrolysisandfreeaminoacidsindigestbysubstrateconcentration、enzymeamounthydrolytictemperaturepSandhydrolytictimeweredeterminedbysinglefactorexperimentim

6、portantfactorswerescreenedbysinglefactorexperimentThebesttechnologyconditionbyCaroidWas“temperature60℃，pH65，concentrationofsubstraet1％，efflzymeamount15％，hydrolytictime30hOnthebaseoftheaboveexperimentalkalineproteinaseWas

7、empolyedtohydrolysisagainFirstlysinglefactorwasemployed，thanthebesttechnologyconditionbyalkalineproteinaseWatq：temperature50。C，pH80，enzymeamount0875％hydrolytictime30hSubstrate’Sdegreeofhydrolysisandcontentofacidsolublepe

8、ptideindigestreached184％and786％respectivelywhichwas56％and302％higherthansingleenzymehydrolizationThedigestWassegregatedbYSephadexG15gelcolumnPolypeptidemolecularweightbetween300—1355Daoccupied413％ofallelutingpeal【areaindi

9、gestbycaroidhydrolizationhigherthan1355Daoccupied38％，lowerthan300Daoccupied207％Polypeptidemolecularweightbetween3001355Daoccupied382％ofallelutingpeal(areaindigestbYalkalineproteinasehydrolizationhigherthan1355Daoccupied4

10、26‰lowerthan300Daoccupied192％Polypeptidemolecularweightbetween3001355Daoccupied526％ofallelutingpeal【areaindigestbYcombinationoftheabovetwoenzymeshydrolizationhigherthan1355Daoccupied315％，lowerthan300Daoccupied159％Highper

11、formanceliquidchromatographyandlaserdesorptiontimeofflightmassspectrometrywereemployedtoanalyzeanddetectethedigestTherehavebeenthreechromatographicpeatretentiontimewas21837min23300minand24499minbyturnsThecontentofmateria