氟桂利嗪和N---乙酰半胱氨酸促進(jìn)PD移植神經(jīng)元存活的實驗研究.pdf

1、東南大學(xué)碩士學(xué)位論文氟桂利嗪和N乙酰半胱氨酸促進(jìn)PD移植神經(jīng)元存活的實驗研究姓名：王育和申請學(xué)位級別：碩士專業(yè)：人體解剖與組織胚胎學(xué)指導(dǎo)教師：曾水林20030401查墮查堂堡主蘭堡堡苧一——FIunarizine／N—acetylcysteineimprovethesurvivalofembryonicdopamineneuronsaftertransplantationintatmodelParkinson’sdiseaseWANGY

2、uheZENGShui娃ⅡDepartmentofAnatomyHistologyandEmbryologySchoolofBasicMedicalScienceSoutheastUniversityAbstractObjectire：toinvestigatetheeffectsofpretreatmentwithflunarizifie(FLU)andNacetylcysteine(NAC)ontheEdratventralmese

3、ncephaiiccellsuspension，whichtograftInto6hydroxydopaminelesionedratMethods：toproduceunilateral6OHDA—lesionedSprague～Dawley(SD)rats，andthen48PDmodelswereselectedandrandomizediritefourgroups(n=12ineachgroup)：grouponeisVMce

4、llsospensionwhichpreparetobegrafted，grouptwoiSpretreatedwithflunarjzine(1pM)，groupthreeiswithNacetylcysteine(25M)，groupfouristreatedwithbothFLUandNACEachgroupwasdividedrespectivelyintothreetimepointsat1，7，14daysfollowing

5、grafted，andtherearefourPDratsateachpointA11ofthegraftedrat；ereassessedrotationalbehavior。thenonegraftedratineachgroupwasusedforFcMdetectionatfirsttimepointOtherswerekilledateachtimepointrespectivelYTherearethreetechnlque

6、ssuchasHEstaining，THimmunohistochemistryandTUNELtechniqueswhichusedtoassesssurvivatcellandapoptoticcelldeathbydetectionofitsbiochemicalandmorphologicidentifiersrespectivelvResult：eachgroupdemonstratedrotationalbehaviorno

7、significantdifferenceslpO05)aftergraftedat1and7daysUpto14daysthealterationwasverysignificantdifferencesbetweencontrolgroupandmedicinepretreatmentgroups(p005)ThenumberofTI]一positirecellsissignificantdifferencesbetweencont

8、rolgroupandmedicinepretreatmentgroups(PO05)Ifurthermore，ineachgroup，therealsohavesignificancedifferencesamongthethreetimepoints(p005)ThroughcountingtheIUNEL—positirenuclei，therealsoweresignificantdifferencesbetweencontro

9、lgroupandmedicinepretreatmentgroups(pO05)andsimilartoamongthethreetimepointsateverygroup‘pO01)ThroughFCMPl／AnnexinVdetectiontherateofapoptoticcelldeathgraftedDAneuronsiS4611％，29，16％，26，12％。2440％onday1Conclusion：(1)Themaj

10、orityoftransplanteddopamineneuronsdieingraitswithinthefirst24hoursaftertransplantand(2)most1ikelybyanapoptoticmechanism(3)flunarizineandNAcetylcysteinemayimprovetheviabilityofDAneuronsgraftedforParkinson’sdisease，(4)ther