結(jié)球甘藍初級三體系的選育及鑒定.pdf

1、河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文結(jié)球甘藍初級三體系的選育及鑒定姓名：祝海燕申請學(xué)位級別：碩士專業(yè)：蔬菜學(xué)指導(dǎo)教師：張成合20050601SelectingandIdentificationofaSetofPrimaryTrisomicsinCabbage(BoleracevatcapitataL1AuthorZhuHaiyanMajorOlericultureTutorZhangChenghe(CollegeofHorticulture，Agr

2、icultureUniversityofHebei，Baoding，China071001)AbstractTheinbredlinesofdiploidandtriploidoftheheadcabbage(zhonggan一11)wereusedasmaterialsforselectingprimarytrisomicsofcabbageinthispaperBycytologicalidentificationandkaryot

3、ypeanalysis，asetofprimarytrisomics(Tri一1～Tri一9)ofthecabbagewasselectedfromprogeniesofthetriploidcrossedwiththediploidparentsTheplantmorphologicalcharacteristics，meiosisbehavior，pollencharacters，seedsetting，transmissionfr

4、equencyofnlfemalegametesandnlmalegametesofthedifferenttrisomicswereobservedorstudiedConclusionswereasfol】owed：1Karyotypeanalysisshowedthatthekaryotypeofthenormaldiploidcabbage(CK)was2n22x21828m10sm(2SAT)Andwiththekaryoty

5、peofthediploidcabbageascontract，bycytologicalandmorphologicalidentificationandkaryotypeanalysis，awholesetoftrisomics(Tri一1～喇9)ofthecabbagewasselectedfromthe612nlplantclonesof3xx2x，inwhichthe3plantcloneswasTri一1，2cloneswa

6、stri一2，5cloneswastri一3，2cloneswastri一4，3cloneswastri一5，1cloneswastri一6，6cloneswastri一7，2cloneswastri一8，3cloneswastri一9，andtheratewas492％，328％，8t9％，328％，492％，164％，984％，328％，492％respectively2Somedifferenceinmorphologicalor

7、developmentalcharactersexistedbetweenthediploidandthetrisomiesoramongthedifferenttrilomicsSuchas，Tri1withmorestrongramificationabilityandtheflowerbranchesslightnessanderection；Tri一2withincompactplantshape，curlyflowerbran

8、ches，andlongerbranchandknote；Tri一3withlongerstyle，theanthersbellowthestyleobviouslytheantheremptyandshriveled；Tri一4withfourridgeflowerbuds，longerflowerstalkandvolutepetal；Tri一5withslighterandcurvedbranches；Tri6withdwarfa

9、ndcompactplantshapeandsub—veinsindistinctly；Tri一7withhighandstrongplantshape，lateanthesis，biggerandroundflowerbuds；Tri一8withfewerandthickbranches，andtheflowerbudsshowingalittlepurple；Tri一9withsmallandthinleaves，andtheant