粉蚧科昆蟲DNA條形碼鑒定技術(shù)及數(shù)據(jù)庫(kù)的建立.pdf

1、 華南農(nóng)業(yè)大學(xué) 華南農(nóng)業(yè)大學(xué) 學(xué)位論文原創(chuàng)性聲明 學(xué)位論文原創(chuàng)性聲明 本人鄭重聲明： 所呈交的學(xué)位論文是本人在導(dǎo)師的指導(dǎo)下獨(dú)立進(jìn)行研究所取得的研究成果。 除了文中特別加以標(biāo)注引用的內(nèi)容外， 本論文不包含任何其他個(gè)人或集體已經(jīng)發(fā)表或撰寫的作品成果。 對(duì)本文的研究做出重要貢獻(xiàn)的個(gè)人和集體， 均已在文中以明確方式標(biāo)明。 本人完全意識(shí)到本聲明的法律結(jié)果由本人承擔(dān)。 作者簽名： 日期：

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4、 日期： 學(xué)科帶頭人簽名： 日期： II DNA Barcoding and Database Construction of Pseudococcidae Ren Jingmei (College of Agriculture, South China Agricultural University, Guangzhou

5、510642, China) Abstract: Mealybug ( Hemiptera: Pseudococcidae ) is a tiny insect group that feeds on plant sap. Its adaptability and the reproductive capacity is very powerful, and easily leads to disaster to agriculture

6、 and forestry areas. Mealybug is high alert pest of our country. With highly covert, it brought great difficulties to our entry quarantine. The rapid and accurate identification is the foundation of effectively monitorin

7、g and controlling to mealybugs. But there are many problems in the morphological indentification method. So looking for more efficient and convenient method of identification is particularly urgent. DNA barcoding is an i

8、dentification method that using sequence differences of molecular markers in species. In this study, we used the 5’ and 3’-end of COI gene to verify the effectiveness of identification in Mealybugs. We acquired 502 seque

9、nces of 5’-end and 356 sequences of 3’-end from 538 mealybug samples. The sequence length of them was 649bp and 820bp. The average contents of AT were significantly higher than GC for the COI sequences in mealybugs. The

10、intraspecific genetic distances range of 5’ and 3’-end were 0%~8.03% and 0%~9.5%. The interspecific genetic distances range of 5’ and 3’-end were 1.6%~22.89% and 1.99%~21.39%. Some overlap was observed in the distributio

11、n of genetic distances among conspecific individuals and interspecific individuals. The variation of different geographic populations in Ferrisia virgata and Phenacoccus madeirensis was very obvious, and had reached a le

12、vel of species. Phylogenetic tree of NJ showed that all species could form an independent clade. Phylogenetic tree of Pseudococcus, Phenacoccus, Phenacoccus and Dysmicoccus in NJ method showed that COI gene can distingui

13、sh the species of main genus in Pseudococcidae, and the result was basically consistent with morphological identification. The identification of closely related species was also effective. COI gene can be used for the ge