體外人骨髓間充質(zhì)干細胞向汗腺細胞表型轉(zhuǎn)化的實驗研究.pdf

1、內(nèi)蒙古醫(yī)學(xué)院碩士學(xué)位論文體外人骨髓間充質(zhì)干細胞向汗腺細胞表型轉(zhuǎn)化的實驗研究姓名：艾麗申請學(xué)位級別：碩士專業(yè)：病理學(xué)與病理生理學(xué)指導(dǎo)教師：李秀霞20100501。內(nèi)蒙古醫(yī)學(xué)院碩士研究生學(xué)位論必(墊!墮盟AnExperimentalstudyofhumanbonemarrowmesenchymalstemcellsconvertingintoPhenotypeofsweatglandcellsinVitroAbstractObjective

2、1Toestablishthemodelofsweatglandcellwhichheat—shockedandtheindirect∞一cultureofhumanbonemalTowmesenchymalsternceUs(MSCs)andsweatglandcellsinvitro；2InvestigatingthechangeofMorphology，Phenotype，GenotypethatbeforeandafterCOc

3、ultureofhumanbonemarrowmesenchymalstemceilsDiscussiononhumanbonenIBlTOWmesenchymalsterncellsdifferentiatetosweatglandsfeasibilityMethods1MSCsandsweatglandcellswereislated，cultured，expandedandidentifiedinvitro：①MSCsandswe

4、atglandceilswereislated，culturedandexpandedsuccessfully；②Usinginve—rtedphasetoCOntrastmicroscopetOobservethecellsmorphology；③UsingirrtmunohistochemistrytodetectimmunophenotypeofMSCsandSWeatglandcells2Indirect130一cultureo

5、fMSCsandsweatglandceils，identification：①Toestablishthemodelofsweatglandcellswhichheat—shockdContinueculturing3—5dToCollectsupematantasconditionedme—diuminducingdifferentiationMSCs；②TocontrastMSCsmorphologicalchangesofoD—

6、cul—tureafter5daystolOdays；③UsingimmunohistochemistryandFlowcytometrytechniquestocontrastMSCsMorphologicalchangesofCO—cultureafter10days；④GenechiptechnologydetectgenotypechangesofMSCsResIllts1TheresultofMSCsandsweatgland

7、cellswereislated，cultured，expandedandidentified：①MSCsandSweatglandcellswereishted，culturedandexpandedsucceasfuUyinvitro；②UsinginvertedphaseCOntrastmicrcscopetoobservecellgrowthingoodCOndition；③DetectedsurfacemarkerofMSCs

8、werepositiveforCU29，CD44，CDl05，sweatglandcellswerepositiveforCK7，CK8、CKl8、CKl9、CEA；2Theresultofindirecting∞一cultureofMSCsandSweatglandcells，identification：①Afterheatshockedwithsweatglandcells，mostofintercellularCOnnectio