低氧及跑臺(tái)訓(xùn)練對(duì)大鼠骨骼肌總蛋白和骨骼肌IGF-Ⅰ基因表達(dá)的影響.pdf

1、南京體育學(xué)院碩士學(xué)位論文低氧及跑臺(tái)訓(xùn)練對(duì)大鼠骨骼肌總蛋白和骨骼肌1GFI基因表達(dá)的影響InfluenceofhypoxiaandtreadmilltrainingtotheconeentrationproteinofskeletalmuscleandGeneexpressionofskeletalmuscleIGFIinrats培養(yǎng)單位—垂盤叢盤生目蟲L—————一學(xué)科專業(yè)—垂薹生生鹽立匕fL——————一研究方向女!I堡墮控焦垂盈絲疸

2、羞生送復(fù)碩士生姓名——j鹽j奠生L—————一指導(dǎo)教師——蘭—!墮——————一南京體育學(xué)院碩Ij學(xué)位論文Influenceofhypoxiaandtreadmi11trainingtotheconcentrationproteinofskeletalmuscleandGeneexpressionofskeletalmuscleIGFIinratsAbstractStudypurpose：(1)Influenceofthehypoxia

3、andtreadmilltrainingtototalproteinoftherat’Sskeletalmuscle(2)tounderstandthechangeruleoftherat’SskeletalmuscleIGF—IgeneexpressionintheprocessofhypoxiaandtreadmilltrainingStudymethods：Randomdivide60maleSDratswhichwerescre

4、enedinto6groups：NC，HC，NEL，HEL，NEH，HEH，tenratseachgroupIntensityis152m／minand268m／minrespectively，inclinationis10degree(NEL)、(NEH)、(HEL)and(HEH)fourgroupsaretrainingundernormaloxygen，thetrainingtimeis40minperday，sixdaysaw

5、eekHELandHEHgroupsarelivingunderlowoxygenTheoxygenthicknessiscontrolledto141％inlowoxygenenvironmentwhichisequalinelevation3000mLowoxygengroupratsliveinlowoxygencanvasfrom8pmeachdayto8amnextdayFourgroupofexperimentalratst

6、ake2weeklongadaptabletrainingand5week—longformaltrainingTakingrats’thighsuralmusclewithin24hoursafterthelasttimetraining，theninterceptthesamepartofthemiddleofmuscle。UsingBradfordmethodtodetermineproteindensityandtestinga

7、bsorbencycancalculatethecontentofproteinUsingWesternBlottingmethodtodetermineIGF—IcontentofmusclethenextractmRNAUsingRT—PCRtoexamineIGF—ImRNAexpressionofeachgroupConclusion：Afterfive—weekhypoxiaexercise，thesportinggroupr

8、ats’bodyweightobviouslydroppedamongwhichtheHEH’SwasthelowestItpromptsthatthelowoxygenmovementhastheinhibitoryactiontotherat’SbodyweightTheNELgroup，NEHgroupratsskeletalmuscleIGFIandthetotalproteinobviouslyelevatedItiScons

9、istenttothereportthatmovementcanpromotetheskeletalmuscleIGF—IsecretionandincreasetheskeletonproteinsynthesisHELgroupelevatedobviouslycomparedwithHCgroup，HEHgrouphadnochangesItpromptsthatthedifferentmovementintensityhasdi

10、fferenteffecttats’skeletalmuscleIGF—IandthetotalproteinNELgroupcomparedwiththecorrespondinggroupelevatedobviouslyonIGF—ImRNATheHELgroupelevatedslightlyandHEHgroupdroppedsignificantlyItpromptsthatitexistsdifferenceofIGF—’