中國蒙古族人群HLA-Ⅱ類基因多態(tài)性研究.pdf

1、中山大學(xué)碩士學(xué)位論文中國蒙古族人群HLAⅡ類基因多態(tài)性研究姓名：張華申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：徐安龍20060501StudyofthePolymorphismofHLA—IIGenesinMongolPopulationofChinaAbstractThehumanleukocyteantigen(HLA)isthemostpolymorphicregioninthewholehumangenomeStud

2、yontheHLApolymorphismofhumanpopulationsmayhelpUStostudytheorigin，evolutionandmigrationpatternsofhumanpopulationsPolymorphismofHLA—DPBl，HLA—DRBlandHLADQBlwasinvestigatedviaPCR—SBT(sequence—basedtyping)methodin87unrelatedi

3、ndividualsofMongolethnicgroupfromChina，and27DRBIalleles，21DQBlallelesand16DPBlallelesweredetectedAmongthe27DRBIalleles，thesixmostfrequentalleleswereDRBl040101(155％)，DRBl030101(103％)，DRBl’090102(86％)，DRBl070101(75％)，DRBl1

4、50101(63％)andDRBI130201(56％)Amongthe21DQBlalleles，thesixmostfrequentalleleswereDPBl020102(293％)，DPBl0401(161％)，DPBl0501(155％)，DPBl0402(126％)，DPBl1701(63％)andDPBl030101(56％)Amongthe16DQBIalleles，thesixmostfrequentallelesw

5、ereDQBl030101(218％)，DQBI0201(201％)，DQBl030302(149％)，DQSl0602(92％)，DQBl050101(86％)andDQBl060101(56％)ThefrequencyofDRBlDQBlhaplotypesofMongolethicgroupWasestimatedandthemostcoonhaplotypeswereDRBl030101DQBI0201(747％)，DRBI04

6、0101一DQBl030101(6_32％)，DRBl090102DQBl030302(517％)，DRBl070101一DQBl0201(460％)andDRBl150101一DQBl0602(345％)TheneutraltestrevealedthatHLAIIDPBl，DRBIandDQBlwereunderthebalancingselectioninMongolethicgroupThephylogenetictreeand