鯽魚PKR-like(PKZ)Zα能將含GC片段的B-DNA翻轉為Z-DNA.pdf

1、南昌大學碩士學位論文鯽魚PKRlike(PKZ)Zα能將含GC片段的BDNA翻轉為ZDNA姓名：盧普忠申請學位級別：碩士專業(yè)：遺傳學指導教師：朱友林；胡成鈺20110608ABSTRACTABSTRACTPKZwasthemostrecentlydiscoveredmemberofeIF2akinasefamilyinfishC口PK乙possessedaconservedeIF2akinasecatalyticdomaininC—te

2、rminalandtwoZDNAbindingdomains(Z0【)inN—terminalThetwOZasubdomainsweretermedZalandZa2，respectivelyTheZalZa2domainactasaregulationdomain，anditsfunctionswerenotSOlucidToinvestigatethefunctionofPKZZaandthelengthandtheprefere

3、ncethatitcanbindtoandfliptoZ—DNA，wesynthesissomeOligoDNA，atthesametime，thetwelvepeptideswereexpressedinEcoli碭einteractionbetweenZapeptideandOligoDNAhasbeeninvestigated、析thCDspectrumWefindFirstTooshrotofthelengthoftheHair

4、pinstoformtheZDNAstructureSecondtheshortestHairpinsthatcaninteract晰t11ZalZc【2isHairpin4，whileHairpin3interact謝mZalZaldramaticlyTtlirdthereareinteractionsbetweenZalZa2OligoDNAthatcontainGCsegmentbutnointeractionsbetweenZa

5、lZa2andOligoDNAwith1’AsegmentandPrimerDNAFourth，Hairpin6canbefliptoZ—DNAbyZalZal，whileZa2Za2CannotWeinvestigatedthenineimportantaminoacidsiteswhichmayinteractwimtheDNAbackboneand(or)maintainthetertiarystructurebytitratio

6、nHairpin6、)l，itllthealaninemutationsandsubstitutemutationspeptidesWefind，F(xiàn)irst，N38A，Y42A，P57AandP58AalmostlostthewholecapabilityofoverturnTheresultindicatedthatN38，Y42，P57andP58aleveryimportantforZalZa2functionsSecond，$3

7、5AandK56AretainpartoverturncapabilitythisresultindicatethatthosetwositesalenotSOimportanttoZalZa2functionsT11irdwefindthattheCDtitrationspectrumofHairpin6、)~，itlltheincreasingK34A，R39A，W60Apeptidesweredifferentfromwithth

8、eWildTypeZalZa2TheintrestingresultssuggestthatW60AmayconvertsHairpin6DNAinacompletedifferentwayincontrast晰ththeresultsofwildtypeZalZa2TounderstandthedifferentphenomenaW60AneedsmorecontinuousinvestigateKeyWords：PKZ；Za；mut