重組類人膠原蛋白HIC-I對BHK-21細(xì)胞影響實(shí)驗(yàn)研究.pdf

1、分類號——密級——單位代碼!Q22學(xué)號—2002—2524西北大學(xué)碩士學(xué)位論文題目重絲差厶蕉盈墨魚旦蘭￡：亙壁曼塾K：至量塑照曼墮塞墜塹塞指導(dǎo)教師整垡豎專業(yè)技術(shù)職務(wù)塾撞一學(xué)科(專業(yè))生物化工答辯日期學(xué)位授予日期二零零五!E五月ExperimentalResearchofHuman—likeCollagenIonBHK21CellGrowthAbstractTheeffectsofHumanlikeCollagenI(HLCI)oncel

2、lgrowthwerestudiedbyBIIK21cellinvitroThefollowingworkshavebeendoneduringthestudy1TheeffectofHLCIOnBHK2lcellgrowthThegrowthcurveofBHK21cellWaScreatedbycellculturedinvitroTheeffectsofvariousHumanlikeCollagenIconcentrations

3、(O1‰，03‰，05‰，07‰，09‰，11‰，13‰)atdifferentculfivatontime(24hr，48hr，72hr，96hr120hr)oncellgrowthproliferationandviabilitywerestudiedbyM1vrAssayTrypanBlueDyecellcountingandstatisticanalysisThepresentstudyshowedthatRPMll640med

4、iainthepresenceofHumanlikeCollagenIconcentrationsat03‰，05‰，and13％0hadsignificantinfluencesoncellgrowth(PO05)HumanlikeCollagenIalsohadpositiveeffectsoncelldivisionmteaftercultured48hrItdemonstratedthatHumanlikeCollagenIha

5、dpositiveeffectsonBHK21cellgrowthatlowerconcentrationsandnosignificantinfluenceonviabilitymtethusshowed900dbioeompatibility2ThemechanismofHLCI011BHK21cellgrowthTheeffectsofHLCIoncellproliferationcellapoptosisroteGl％，G2％，

6、S％ofcellgrowthandmorphologyat24llrwerestudiedbyMTTAssayLDHanalysis，F(xiàn)CMandmorphologyn圮effectsonBHK21at24hrshowedthatRPMll640mediainthepresenceofHumanlikeCollagenIconcentrationsat02‰，03‰，hadpositiveeffectsoncellgrowth(P005

7、)。buthadnosignificantinfluencesonLDHcontentinculturemediaFCManalysisdemonstratedthatHLCI(01‰，O2‰，O3‰，w／v)madeaslightchangeoncellapoptosisrateandHLCIhadnosignificantdifferencebetweencellgrowthinG1％，02％，S％andthatofblankcon