北方黃胡蜂和德國(guó)黃胡蜂（工蜂）腦中5-HT能神經(jīng)元系統(tǒng)免疫細(xì)胞化學(xué)定位研究.pdf

1、東北師范大學(xué)碩士學(xué)位論文北方黃胡蜂和德國(guó)黃胡蜂（工蜂）腦中5HT能神經(jīng)元系統(tǒng)免疫細(xì)胞化學(xué)定位研究姓名：李霞申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：動(dòng)物學(xué)指導(dǎo)教師：暴學(xué)祥20050501AbstractApplyingColophonyPamffin(CP)tissueembeddingtechniquecombinedwithimmunocytochemicalStreptavidinPeroxidase(SP)method，wehadinvestig

2、atedthedistributionofserotonin(5HT)immunoreactiveneuronsystemintheprotocerebrumofVespulatufatufaandVespulagermanicaResultsshowedthatthebrainofwaspsisdeveloped，andtheprotocerebrumisthelargestinthebrain，almostoccupyingthre

3、efourthsofthewholebrainvolumeThebrainstructuresaresimilarinthetwowasps，andtheprotocerebrumiscomposedofseveralobviousstructuredneuropilsincludingthemushroombody(Ma)，centralcomplex(CC)，opticlobes(OL)，lateralaccessorylobe(L

4、AL)，andanterioroptictuberclerAOTu)MushroombodyispairedandsymmetricaldistributedOpticlobeisthevisioncenteroftheinsect，whichconsistsofthreeconcentriczones，thelamina，medull如andlobulaSerotoniniswidelydistributedthroughoutall

5、areasofthebrainThenumberofserotoninlikeimmunoreactivesomataintheprotocerebrumisabitsmall，andthepositiveneuronsaremainlysymmetricallylocalizeddorsalorposteriortotheprotocerebrumInadditionabout74and76serotoninpositivesomat

6、aclusteringinto8groups，arerespectivelyobservedintheprotocerebrumofVespulatufa，咖andVespulagermanicaInboththetwowasps，theserotoninimmunostainingintheanterioroptictubercleisweakerthanthatinthesurroundingneuropilsHoweveritis

7、muchstrongerinVespulatufarufathanthatinVespulagermanicaIntenseserotoninimmunoreactivitycanbefoundinthepedunclesofmushroombodies，thelateralaccessorylobes，thelateralneuropilsofprecerebralbridge，andthelargeregionofthecentra

8、lbodyanditssurroundingneuropilsMoreover，theserotoninimmunoreactivevaricosefibresformthickneuralnetworkinthecentralbodysurroundingneuropilsandthesuboesophagealganglion(SOG)Therearenotableserotoninpositivefibreprojectionsi

9、nthecalyces，opticlobes，andantennallobes，buttheKenyoncellsandthepostcerebralbridgearenegativelylabelledTheserotoninimmunoreactivityinthedorsallobes，tritocerebrum，andsuboemphagealganglionisrelativelystrongTheserotoninposit

10、iveneuronsinthesuboesophagealganglionsendmostprocessesintotheneuropil，wheretheyformafewthickfibretractsOnthewhole，noobviousdifferencesofserotonindistributionpatternaredetectedintheneuropilsbetweenthetwoinsectspecies，whil