蘇云金芽胞桿菌YBT-1520熱脅迫下的生理變化及差異蛋白質(zhì)組學(xué)研究.pdf

1、華中農(nóng)業(yè)大學(xué)博士學(xué)位論文蘇云金芽胞桿菌YBT1520熱脅迫下的生理變化及差異蛋白質(zhì)組研究姓名：吳丹丹申請(qǐng)學(xué)位級(jí)別：博士專(zhuān)業(yè)：微生物學(xué)指導(dǎo)教師：喻子牛；李明順201106華中農(nóng)業(yè)人學(xué)20ll屆博士研究生學(xué)位論文Abstract占臼cf肌岱砌咿西呼P玎s括∞f)hasbeen謝delyusedfor50yearSasasafebiopesticideforcontrollingagriculturalaIlds越ta巧insectpests

2、becauseofitsinsecticidalcrystalproteins(ICPs)ThestrainusediIl血isstudyw雒丑肪緲以g鈀塒豇subsp覷邪細(xì)舫YBTl520，whichdemons仃ateslli曲iIlsecticidalactivitiesagainstZ印翻叩倫朋nlaⅣaeYBT1520waSorigimllyisolatedaIldch磁Icterizedinourlaborato珥In“ss

3、tudyaproteotIlicapproachconbinedwithphysiologicalinVestigationwasusedt0describeⅡleproteo耐c孤dphysiologicalchangesaIldt0reVealthesurviValstrategiesof歷YBTl520uIldefalong—temheats晚sscondition(420C)ThecI娜gesofmeintraCellularp

4、roteomeunderheatsnesswerezusfollows：(1)someoftlleheatshockproteiIlsofcl祁sIaIldII(DnaK，QoEL，GrpEaIldClpC)werestillinducedunderlong—termheats仃ess；(2)mreerlonclassicalheatshockproteins(BDHl，Gu扭a11dGST)werealsoinduced岫derlon

5、g—temheats仃ess，theycouldberegarded嬲tllespecificfactorsoflong—temlheats缸ess；(3)83％ofmedifferenti2Lllyexpressedin仃acellularproteinsdecreasedillcludingtoxinproteins，potentiaLltoxinprotems，proteaSesanden巧nlesinVolVedintlleb嬲

6、icmetabolicp利【l_waysAllmesedecreaSeproteins、Ⅳereprobablyduet0ⅡleproteolysisprocessofC1pCa疵№rⅡ1estringentresponses仃iggeredbyheatstress；(4)abom50％oftIledecre2usedproteinswererecovcredatⅡlelatestageofheats仃essconditioIl，ind

7、icatingt11atmeheatg吐essedcellsgamedⅡles仃esssadaptiveabili夠somehowHeats仃essmainlyinfluencednlecba船deristicsofYBT_15200nfouraspects：(1)ttleabil時(shí)ofsynmesisofICPsa11doⅡ1erpote而aJpattlogenicfactors、ⅣereloStorsigrlificamlydecr

8、eased；(2)celladhesiona11dmotili夠wercalsolost；(3)mecellsdidnotspomlate，(4)cellskeptaccumulatingpolyp_hydro礙bu妙rate(PHB)ThesuⅣ沁als位此giesofYBTl520underlong—temlleats骶sswere弱follo粥Thefirst蛐g(shù)y：meiIlductionof110ncl嬲sicalheatsh