應(yīng)用于生豬飼養(yǎng)的益生微生物的篩選及其生物學(xué)性能評價.pdf

1、學(xué)位論文獨創(chuàng)聲明學(xué)位論文獨創(chuàng)性聲明本人聲明所呈交的學(xué)位論文是本人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果。據(jù)我所知，除了文中特別加以標(biāo)注和致謝的地方外，論文中不包含其他人已經(jīng)發(fā)表或撰寫過的研究成果，也不包含為獲得南昌大學(xué)或其他教育機構(gòu)的學(xué)位或證書而使用過的材料。與我一同工作的同志對本研究所做的任何貢獻(xiàn)均已在論文中作了明確的說明并表示謝意。學(xué)位論文作者簽名(手寫蘭嘉拇蕨簽字日期：夕口11年I1月力轤學(xué)位論文版權(quán)使用授權(quán)書本學(xué)位論文作者完

2、全了解直昌太堂有關(guān)保留、使用學(xué)位論文的規(guī)定，有權(quán)保留并向國家有關(guān)部門或機構(gòu)送交論文的復(fù)印件和磁盤，允許論文被查閱和借閱。本人授權(quán)直昌太堂可以將學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進(jìn)行檢索，可以采用影印、縮印或掃描等復(fù)制手段保存、匯編本學(xué)位論文。(保密的學(xué)位論文在解密后適用本授權(quán)書)學(xué)位論文作者簽名(手寫臻拇炙導(dǎo)師簽名(手寫)：簽字日期。山Jf年ff月砷日簽字日期2幽，J年痔Etff月列l(wèi)■1^』p。，7。嶧njI；^一id鼉Abstr

3、actAbstractChinaisthelargestcountryrmsingpigsintheworldAseffectivefeedingadditives，antibioticshavebeenwidelyusedinpigraisingstomeettherequirementsofintensiveandlargescalelivestockbreedingHoweversomeadverseeffects，egdrugr

4、esidues，teratogenesis，carcinogenicmutagenicareemergingcomparedwimtheantibioticabusing，whichmakestheecologicalpigraisingmethodtobetheresearchfocusEcologicalpigraisingmethodreferstoreplacingtheantibiotics誦tllprobioticmicro

5、organismswhenraisepigs，includingtheprobioticspreparationforfermentationbedInrecentdecades，thismethodhasbeenappreciatedbymostresearchersforitsbeneficialeffects，suchasgrowthpromotingwithoutsideeffects，noresidualcontaminati

6、onandnodrugresistanceInordertoinvestigatetheeffectsofprobioticmicrobesonfermentationbedseveralselectedmediaWereusedfortheisolationofmicroorganismscontaininginmicrobialecologicalagents，and6isolateswereobtainedaccordingthe

7、irclonemorphologycellmorphologythenthePCRDGGEtechnologywassubsequentlyusedtoclassifythemandonlytwodifferentstrains，named雒LactobacillusplantrumandCandidatropicalisWereidentifiedInnextstep，theeffectsofthepH，temperature，bil

8、esalts，copperaerobic／anaerobiconthevialblecellcountingofisolatesWereinvestigatedOurresultsshowedthattheLactobacillusplantrumcan’tgrowwhenthepHisblow25orthecopperionconcentrationishigherthan150ppm，whilethenumberofCandidat

9、ropicalisreceiveslittleimpactbysuchfactorsInaddition，theamountsofCandidatropicalisreduced6magnitudesat42“12comparedwiththatat37℃Also，wecheckedtheimpactsofdifferentantibiotics(amoxicillinoxytetracyclinehydrochloride，metha

10、cholinequinoline，olaquindox，clarithromycin，ciprofloxacin，oxytetracycline)onthegrowthofprobioticsandpathogenscommonlyexistedinfermentationbed，andtheconcentrationsofantibioticsWeredeterminedaccordingtotheirfeedingqualityan