鄰苯二甲酸二(2-乙基己)酯單克隆抗體的制備及ELISA檢測方法的建立.pdf

1、鄰苯二甲酸二(2乙基己)酯單克隆抗體的制備及ELISA檢測方法的建立PreparationofMonoclonalAntibodyagainstPhthalateesters(2一ethylcaproic)andEstablishmentofanIndirectCompetitionELISAMethod研究生：馮云飛導(dǎo)師：王捍東教授學(xué)科：臨床獸醫(yī)學(xué)MSCandidate：FengYunFeiSupervisor：ProfWangHan

2、dong揚(yáng)州大學(xué)獸醫(yī)學(xué)院臨床獸醫(yī)學(xué)教研室2013年6月?lián)P州大學(xué)碩士學(xué)位論文IIABSTRACTPhthalate(2ethylcaprice)phthalate(DEHP)belongtotheophthalmicacidestercompounds，fromlargeoutput，increasingthematerialsoftnesscharacteristicsofgood，cheap，widelyusedinplasticpr

3、oductsThiskindofmaterialisakindofenvironmentalhormone，areclassifiedassuspectedenvironmentalhormones，highdosesofDEHPinthehumanbodycantcompletelymetabolism，causingaccumulationforalongtimetostayinthehumanbodycancausesideeff

4、ects，harmtohealthTheEuropeanUnion，theUnitedStatesenvironmentalprotectionagency(pea)hasalreadypassedsomelawsandregulationstostrictlycontroltheplasticizertestingstandard，andarenowusingcitricacidesterepoxyacidesterssuchasse

5、curityenvironmentalprotectionplasticizerdoupdatesubstituteproductsSomeregulatorymeasuresnOWbeingdevelopedinChinaDEHPhasestablishedavarietyofdetectionmethodsathomeandabroad，suchashighperformanceliquidchromatograpHy(HPLC)，

6、gaschromatograpHy(GS)，gaschromatograpHymassspectrometry(GCMS)；themethodisaccurate，sensitiveandeffective，butallneedexpensiveinstrument，professionaloperationskillsandcomplexsamplehandlerAndELISAmethodCansolvetheseproblems，

7、itisasimple，accurate，efficient，cheap，andspecificdetectionmethod，suitableforrapidscreeningofbulksamplesThisstudywithmonoclonalantibodyandenzymelinkedimmunosorbentassaytechniqueasthefoundationthroughtheproteinsynthesisofar

8、tificialantigenofDEHPconnectiontechnologypreparationofthehighsensitivitystrongspecificityhightiterofantibodyandestablishtheDEHPCeliadetectionmethod1DEHPsynthesisandidentificationofartificialantigensThroughfirstafteraddin

9、gnitroDEHPagainthroughthediazolemethodcouplinglinkswithproteins，preparationofartificialantigenofDEHPBSAandDEHPHSA，theultravioletscanningandimmunologicalmethodcouplingsuccessfulpreliminaryidentificationDEHPBSAasimmunogeni

10、c，DEHPHSAasthepackageisoriginal2DEHPsinglepreparationUsedDEHPBSAroutineimmunizationofBALB／cmice，afterfivefreeserumtiter1：12000aboveByhybridismtechnologyandenzymelinkedimmunetechnologyCalllast1strainscreeningforstab1eprod

11、uctionofDEHPspecificitymonoclonalantibodyhybridism，oneofthe4C2straincellssecreteantibodiestoDEHPexhibiteds仃ongcombiningabilityusingtheinvivoinducedbod％the4C2hybridisminjectedintoabdominalcavityofmice，preparationofDEHPasc