PEG-Ha-MEL的制備及其評價.pdf

1、武漢理工大學碩士學位論文PEGHAMEL的制備及其評價姓名：姜曉曄申請學位級別：碩士專業(yè)：藥學指導教師：許沛虎；徐海星201206武漢理工大學碩士學位論文AbstractMelphalan(MEL)iswidelyusedinthetreatmentoftumors，whichisusedinovarian啪cerandbreastcancer，butalsotherearesomedefects，suchas：poorselectiv

2、itypoorresistanceandshorthalflifeAtargeteddrugdeliverysystemWasconstructedinthisdissertationwhichwastoimprovethedefectsmentionedaboveandinbanceitstherapeuticeffectagainstova_dancanccrndstudiestheroleofdrL蟹relaledandperfo

3、rmanceevaluationThemaincontentsofthisstudyaresummariz甜asfollows1Ithasbeendeterminedthatthesyntheticroute、itsoptimaleonditiomandthebestsynthesissolution：Modificatedhyaluronicacidwitllactivatedpolyethyleneglycol(PEG)Thencr

4、osslinkedhyaluronicacidascarrierConnectedmelphalanwithPEGHAMEL，whichistoimprovethedrugefficacintumorcellsAnd1HNⅣ瓜andIRwasusedtoindicatethestructureofproduct2Structuresoftheproduct’Smolecularweightsweredeterminedbygelperm

5、eationchromatography(GPC)AndthereleasedpaclitaxelWasmeastredbyHPLC，andstudiedtheaffectionofpH，rotationspeedandsamplevolumeonthereleaserateBothmethodshavegoodprecisionandaccuracyTheresultsshowthatduetotheenzymewasverysens

6、itivetopH，thereleaseWaShighintheslightlyacidic，andwhichwaslossactivityinthealkalineenvironmentAndtheimpactofthespeedandsamplevolumeonthereleaseisnotgreat3flHNMRandelectronmicroscopy(SEM)indieatethemicelleshadacore—shells

7、tructure，inwhichpaclitaxelwasenvelopedintheCOreThesizesoftheselfassemblymicellesoftheamphiphilicbloekcopolylnersandtheeffectswerestudiedbydynamiclightseattering(DLS)ThenthefactorsaffectingitwerestudiedTheresultsshowthat：

8、thedosagewillaffecttheparticlesizeofthemicelles；beforeorafterfrozentherewerealittlechangeinmieellarsize4CellcultureexperimentsandMTTmethodwasappliedtostudytheinhibitionofmelphalanandPEGHA—MELmicellesOiltumorcellsTheresul