功能化二氧化硅納米顆粒介電泳行為及其對(duì)細(xì)胞介電性質(zhì)影響的研究.pdf

1、湖南大學(xué)碩士學(xué)位論文功能化二氧化硅納米顆粒介電泳行為及其對(duì)細(xì)胞介電性質(zhì)影響的研究姓名：宣鋒申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物醫(yī)學(xué)工程指導(dǎo)教師：王柯敏譚蔚泓20100501功能化二氧化硅納米顆粒介電泳行為及其對(duì)細(xì)胞介電性質(zhì)影響的研究AbstractPrecisefabricationofcontrollableandreversiblenanostructuresormicrostructuresbasedfunctionaldeVicesan

2、dsystemsareofgreatinterestforexpandingthepotentialnanotechjl0109yapplicationsintheareassuchasbiosensingDielectrophoresis(DEP)，themoVementofpolarizableparticleinducedbyanonhomogeneousACelectricfield，hasbeenproVenasarobust

3、methodtorapidlymanipulateandassemblebiologicalandsyntheticnanomaterialsintonanodevicesandmocrosystemsHoweVer，duetoinadequateunderstandingofDEPmechanisms，itremainsVer)，limitedtopreciselycontrolthe6nalstructureofassembledn

4、anoparticlesunderDEPThisthesisisaimedatDEPassemblyoffhnctionalizedsilicananoparticlesanditsefkctsondielectricpropertiesofcellsPreciselycontrollableandreVersibleDEPassembledsubmicrostructhaVebeenachieVedbymodificationofan

5、ionicgroupsonsilicananoparticles，andtheefIfectsofintracellularfhnctionalizedsilicananoparticlesondielectricpropertiesofcellshaVebeenproVenThisthesisiscomposedofthefbllowingthreeparts：1Researchonef免ctsofdifferentsurfacefu

6、nctionalizedgroupsonDEPassemblybehaViorofsilicananoparticlesTheDEPassemblybehaVioroffiVetypesofsilicananoparticles(SiNPs)，includingOH—SiNPs，C00H—SiNPs，NH2一SiNPs，CH3HP02一SiNPsandPEG—SiNPshaVebeeninVestigatedrespectiVelyIt

7、wasfbundthatanionicgroupscouldobViouslyenhancetheDEPassemblybehaviorofSiNPsonthemicroelectrodesTheanionicgroupcouldbringanincreaseonthedielectricconductiVityoftheSiNPsbyenhancingthesurfaceconductiVityTheseresultswellindi

8、catedthattheDEPassemblybehaviorofnanomaterialsinaqueoussuspensionscouldbeenchangedbyfIunctionalizedgroupmodi丘cation2Researchoncontr011ableandreversiblesubmicrowiresassembledfromCH3HP02一SiNPsOnbasisoftheworkinthefirstpan，

9、theDEPassemblybehaViorofCH3HP02一SiNPswasfurtherinVestigatedByusingRubpydyedopedinthecoreoftheCH3HP02一SiNPs，theassemblyprocesswasVisualizedrealtimebyinversefluorescencemicroscopyPrecisecontroloVerthefrequencyoftheappliedA