陸地棉纖維發(fā)育相關(guān)基因Ghkob的克隆及功能分析.pdf

1、中國農(nóng)業(yè)科學(xué)院碩士學(xué)位論文陸地棉纖維發(fā)育相關(guān)基因Ghkob的克隆及功能分析姓名：郭惠明申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：王志興20050601中國農(nóng)業(yè)科學(xué)院碩士學(xué)位論文英文摘要量曼墨囊皇曼曼葶曼曼皇舅■皇皇篁量曼皇墨皇曼毫曼暑鼉罾皇曼高I——曼曼曼曼量寫量曼暑!!孽鼉AbstractCottonisanimportantindustriaIcropworldwide，occupyingimportantpositio

2、ninnationaleconomyInordertoimprovethequalityofrawcoRoninoBrcountryandcompetitivenessintheinternationalmarketoffabrics，itisnecessarytodevelopvarietiesoffinecottonthatcombinedifferentqualitycharacters，suchaslength，strength

3、andslendertomeetdomesticandinternationalmarketdemand，TheKobgeneencodesakindofprotein，whichthecellulosesynthesisneedsItrelatestocellulosearrayandaffectsthecellelongationsubsequently~especiallyinthefastelongatingcellWhenth

4、eceIlulnsesynthesisisbtockedinmutant，thecellulosesuddenlyreductionwillcausethemicrofibertoarrangerandomAsaresult，thepheno可peofplantisdwarfishInthisresearch，theuplandCOttoavarietySHIYUAN321wasusedasamaterialandRACEtechnol

5、ogyappliedinobtainingthewholeeDNAsequenceofthegeneGhkobtotally2001bp，hasabiggestopenreadingframe(oRF)1620bpIts5’untranslationareas(5’UTR)has64bp，andits3’untranslationareas(3’uTR)has317bpTheORFencodesaproteinof539aminoaci

6、ds、andthemolecularweightoftheproteinis60_8kDThededucednucleotidesequenceandtheaminoacidsequenceofGhkobarehighlyhomologoustotheotherplant’SkobTheRNAivectorpAR8containsthefragmentofphytoenedesaturasegenewasconstructedandtr

7、ansferredintotobaccotoevaluatetheeffectivenessoftheRNAivectorAf【erinductionwithethan01theyoungleavesoftransganictobaccoshowedalbinoThen，theRNAivectorBar2，whichharboringtheeDNAfragmentofGhkob，wasconstructedandtransferredi

8、ntocottonWegot43transformantsandvalidatedthemthroughPeRTheovulesoftransgeniccoRonwereculturedinvitroandinducedwithethan01ThedevelopmentoffiberWasinhibitedaaerthesilencingofGhkob，whichmeansGhkobisessentialinfiberdevelopme

9、ntTheoverexpressedvectorpG0229kobwhichharboringtheGhkobgenedrovedby35SpromoterwasconstructedandtransferredintocoRonSeleetioosfortransgenicplantsarecarryingoutInordertounderstandthefunctionofthisganewithinshor￡time，wealso

10、constructedyeast((Schizosaccharomycespombe)vectortoanalyzethegenefunction，buttheresultinvestigatedthatOverexpressionofthisgenecouldnotincreasethelengthofyeastcellItsuggestedthatexpressionofthegeneisnotadirectreasonincell