食用向日葵種質(zhì)資源基礎類群劃分研究.pdf

1、內(nèi)蒙古農(nóng)業(yè)大學碩士學位論文食用向日葵種質(zhì)資源基礎類群劃分研究姓名：郭樹春申請學位級別：碩士專業(yè)：作物遺傳育種指導教師：安玉麟李素萍20080501StudyontheClassificationofC矗fectionSunflower(HelianthusannuusL)AbstractInthisstudythegeneticdiversityin50ConfectionSunflowergermplasmfromdifferentc

2、ountriesorregionswasassessedusingmorphologicalcharacteristicsandRAPDmarkers，andthegeneticrelationshipamongthegermplasmwasrevealedbyclusternalysisatvariouslevelThemainresultswereasfollows：1Thegeneticdiversityamong50Confec

3、tionSunflowergermplasmwasrevealedbyclusteranalysison12morphologicalcharacteristicsTheeuclideandistanceofgennplasmvariedfrom8to360，itsuggestedthatthegeneticdiversityamonggermplasmwaswideClusteringanalysisindicatedthat50ge

4、rmplasmcouldbedividedintothreegroups：27germplasmwereclassifiedintogroup1，group2had22germplasm，andgroup3had1gennplasmClusteringanalysisonmorphologicalcharacteristicswasassociatedwithgeologicaldistribution2OptimizedRAPDrea

5、ctionsystemandsuitableconditionswereobtained3Thegeneticdiversityamong50ConfectionSunflowergermplasmWasanalyzedbyRAPD(RandomAmplifiedPolymorphicDNA)100primerswereusedforamplification，amongwhich19primerswereselectedforRAPD

6、analysisTheseprimerscouldamplifystableandpolymorphicRAPDbandsandproduced123bandsOfthem，150bands(8200％)werepolymorphicThegeneticdifferentindexesofallthegermplasmvariedfrom08122to0214，suggestingthatthegeneticdiversityamong

7、germplasmWaswildlyThedendrogramconstructedusingtheUPGMAanalysissuggestedthat50germplasmcouldbedividedintothreegroups：26germplasmwereclassifiedintogroup1，group2had23gemiplasm，group3had1germplasm，ClusteringanalysisonRAPDma

8、rkersWaSassociatedwithgeologicaldistribution4ClusteringanalysisbetweenmorphologicalcharacteristicsandRAPDmarkerswasveryconsistentKeywords：ConfectionSunflower；Characteristics；RAPD；ClassificationDirectedby：BoffinANYuIinAss