發(fā)酵法提高蛋白飼料利用率的研究.pdf

1、江南大學碩士學位論文發(fā)酵法提高蛋白飼料利用率的研究姓名：楊旭申請學位級別：碩士專業(yè)：發(fā)酵工程指導(dǎo)教師：陸健曹鈺20080601江南大學碩士學位論文AbstractThestudiesofimprovingtheutilizationofproteinfeedwereveryinterested，especiallyinthecurrentcomparativeshortageoffeedproteincircumstancesHowto

2、improvetheefficiencyoffeedutilizationWaSofamoreworthyofattentionTheproductionofhighqualityproteinsoybeanmealfeedbysolidstatecultureWaSthemaindirectionathomeandabroadThesolidstatecultureofsoybeanmealthroughsingleormixedst

3、rainsandfeedingexperimentswerestudiedinthispaperTheproductionofhighlysineandhigh—proteinfermentationsoybeanmealWaSstudiedAhighproteincontentofyeaStstrainWaSobtainedwhichwasidentifiedasPichiaanomalaThroughthesinglefactora

4、ndoptimizedfermentationconditions，thetureproteincontentWaSashighas4387％，whichWaS1118％higherthanrawmaterialsoybeanmealAyeaStstrainwithhiighlysinecontentWaSobtained，whichWaSidentifiedasKluyveromycesmarxianusItWasinducedwit

5、hscreeningalargequantityofmutantswithAEC，andseveralbranchesofyeaStwereobtainedwithrelativehighcontentofintracesslysineItWasconfirmedthemutantKluyveromycesmarxianus局jhashi【曲contentof357％throughfivetransferringexperimentTh

6、econtentoflysineWaS15870％higherthanthatoftheoriginalstrainThroughthesinglefactorandoptimizedfermentationconditions，thelysinecontentoffermentedsoybcanmealreached317％，whichWaS2383％higherthanrawmaterialsoybeanmealThroughthe

7、mixedsolidstatecultureofsoybeanmealwithKluyveromycesmarxianusandPichiaanomalathesinglefactorandoptimizedfermentationconditionswereinvestigatedThecontentsoflysineandproteinwerethemaintargetsTheoptimalinoculationratiooftwo

8、strains，inoculationsize，theratioofrawmaterialsandwaterfermentationtime，theconcentrateofammoniumsulfateandglucoseandfermentationtempertureWaSsetat1：0965，622％，1：1016，4908h，214％，1％and3119℃Thecontentoflysinereached326％，which

9、WaS2734％higherthanrawmaterial。Underthesamesituations，thetureproteinreached4553％，whichWas1538％higherthanrawmaterialTheaminoacidcontentoffermentedsoybeanmealWaSgreatlyimprovedTheresultsoffeed—inexperimentshowedthatdailywei