甲基對(duì)硫磷壓電免疫傳感器的研制.pdf

1、中國農(nóng)業(yè)科學(xué)院碩士學(xué)位論文甲基對(duì)硫磷壓電免疫傳感器的研制姓名：盧澄申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：農(nóng)產(chǎn)品加工及貯藏工程指導(dǎo)教師：潘家榮20080501AbstractMethylparathionisakindofvirulentorganophosphomspesticide，whichhasbeenforbiddenintheUSeofvegetablesandfruits，butisstillallimportantroutinedete

2、ctingpollutionintheagriculturalproductsentrancetradeandthesupervisionanddetectionforthefoodandenvironmentsafetyThisarticletriedtodevelopapiezoelectricimmunosensorforrapidandsimpledetectionofmethylparathionthroughdifferen

3、tdetectionmethods，providingtechnicalsupportforrapidandonthespotdetectionandsupervisionofpesticideresiduesinvegetablesandfruitsThekeypointofdevelopingthepiezoelectricimmunosensoristoimmobilizetheantibodyontothegoldenelect

4、rodeofthequartzcrystal，thearticlecomparedtwoimmobilizationmethods，includingproteinA(SPA)andselfimmobilizedmembrane(SAMs)methods，theconclusionis：comparedwithSPAmethod，moreantibodyCanbeimmobilizedusingSAMsmethod，aswellasth

5、estabilityinconditionthationconcentrationislessthan20mmol／LpHrangeis7074Threemethodshavebeenstudiedwhenmethylparathiondetected，includingdirectmethod，competitivemethodandenzymecatalystaggregationamplifyingmethodForthedire

6、ctmethod，thelinearityscopeofdetectingmethylparathionis10—25llg／mLwithinwhichtwoconcentrationofmethylparathionhasbeenretestedforfivetimeseach，withtherelativestandarddeviationoffrequency47％、53％，anddetectionlimits47％、53％res

7、pectivelyThecompetitivemethod，whichbasedonthecompetitionbetweentheOVAconnectivemethylparathionandthedissociativemethylparathionforthereactionoftheantibodyimmobilizedonthegoldenelectrodeofthequartzcrystal，hasadetectionran

8、geof0110lag／mEandthedetectionlimitof0074Itg／mLThreeconcentrationofmethylparathionwithinthedetectionrangehasbeenretested，thestandarddeviationofwhichare5944％、6900％、9517％(n=7)AddedreclaimedtesttOthesampleoftapwatersoilandve

9、getableshasheenstudiedwiththereclaimedratioof9071011％、938—1093％、719961％EnzymecatalystaggregationamplifyingmethodhasbeenstudiedfordetectionofOVAconnectivemethylparathionAstheantibodyenzymemarkedantibodyreactantandsubstrat

10、eadded，thefrequencyreduced，whichmagnifiedthesignalThedetectionrangeofOVAconnectivemethylparathionis0145ug／mLThreemethodsfordetectionmethylparathionhavebeenstudied，theimmobilizationmethodandthedetectionmethodhavebeencompa