過敏模型的建立及免疫抑制預(yù)防治療跳蚤過敏性皮炎的研究.pdf

1、Y938331分炎號(hào)密級(jí)單化代碼學(xué)號(hào)飛蛾彥意夭等‘碩士學(xué)位淪文10019S030576過敏模型的建立及免疫抑制預(yù)防治療跳蚤過敏性皮炎的研究BuildingamodelforallergydermatitisandInvestigationOilpreventingandtreatingfleaallergydermatitiswithimmunosupression研究生：趙琳指導(dǎo)教師：王賓教授申請(qǐng)學(xué)位門類級(jí)別：理學(xué)碩士專業(yè)名稱：生堡堂

2、研究方向：僉塑痤堂所在。學(xué)院：生物學(xué)院一零零六年六月AbstractAllergydermatitisisrecrndescent，prurific，inflammatoryskindiseases，whoseincidenceincreasesyearlyandharmisalsoincreasinglyconspicuousItisrankedasoneofcombatdiseasesinthe2lslcenturybyWHOFle

3、aallergydermatitis(FAD)isthemostcommonskindiseaseswhichbadlyharmtodomesticcatsanddogsInductionofallergicdermatitis，animmunologicdisorder，inanimalisconsideredasachallengeWereportherethattheinductionofallergicdermatitisinm

4、icewasachievedwithaconsistentclinicalsignofalopeciaandhistopathologyconfirmationbytwoadministrationsoffleaantigensemulsifiedincompleteFreund’Sadjuvant(CFA)intoC57BL／6miceBycomparisonofdifferentstrainsofmice，differentrout

5、esofinjection，differenttypesofadjuvantanddifferentdosagesoffleaantigens，C57BL／6micewerefoundtobemostsusceptibleto100IxgoffleaantigensemulsifiedinCFAadministeredintramuscularlyanddevelopedanexcoriationsandlocalalopeciaThe

6、leveloffleaantigensspecificIgEinC57BLl6miceafterinductionofclinicalonsetswassignificanthigherthanmicewithoutclinicalonset，whichsuggeststhatfleaantigensspecificlgElevel，iscorrelatedwithseverityofallergichyperreactionLevel

7、sofCD4TlymphocytesandlL4butnotIL10norIFHyarethepredominantcytokinesassociatedwiththeclinicalonsetofsymptomsinC57BIJ6miceFurthermore，histopathogicanalysisindicatesthatmastcellsinfiltratedandwereexhibitedtobeatthestageofde

8、granulationintheareaoflesionalskinfromtheinducedC57BL／6miceInconclusion，thefirstrodentallergicdermatitismodelinducedbyfleaantigensemulsifiedinCFAmayprovideanimportanttoolIostudyvaccinesorimmunodulatorydragsforallergicrel

9、ateddisordersandbeneficialtoothersimilarstudiesintheareasofallergiesBasedonourrecentfindingthatthec0一immunizationDNAandproteininducingimmunesuppression，wedeterminedwhethercombinationsofDNAencodesafleaantigenasDNAvaccinea

10、ndthesamefleaproteinantigenasproteinvaccinecaninducesfleaspecificTcellsuppressionSuchsuppressionleadstopreventorcotetheFADsubsequentlyFleaDNAandproteinantigewereco—immunizedintomice，rabbits，cats，wefoundthatallergicreacti

11、onswereinhibited；Tcellsproliferationweresuppressed；theleveloflgEinserumwasdecreased；thelevelofinflammationcytokinesIL4、IL5、1L13weredeclined，butILIOandIFN—Ywereincreased，suggestingthatthissuppressionisassociatedwithhighle

12、velofIL10andgenerationofTregcellsInordertostudythemechanismoftheimmunesuppressioninducedbyCO—immunizationofthefleaDNAandproteinantigenvaccines，wehaveadoptivetransferredandmixedlymphocytereactionsandfoundthattransferredTc

13、ellspossessedthesuppressionfunctionTheseresultsindicatedthatCO—immunizationoffleaDNAandproteinvaccinesinducedregulatoryTcellswhichsuppressallergicreactionsKeywords：Allergydermatitis，animalmodel，Co—immunization，ImmuneSupp