根瘤菌與大豆、土壤間相互適應(yīng)性研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文根瘤菌與大豆、土壤間相互適應(yīng)性研究姓名：房春紅申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：許修宏20070620StudiesonAdaptabilityofRhizobiumtoSoybeanandSoilAbstractSymbioticnitrogenfixationactionofRhizobiumandleguminousplantisthemosteffectivenitrogenfixationsys

2、temwiththehighestamontnitrogenfixedinnatureThemaininfluencefactorsfornodulationandnitrogenfixationofRhizobiumincludesoil，RhizobiumandleguminousplantRhizobiasuitablefordifferentsoybeanvarietiesanddifferentsoiltypesfrommai

3、nproductionareaofsoybeaninHeilongjiangprovinceweresearchedtoscreenthebestcombinationofRhizobiumwithsoybeanandsiol，atthesametime，providingthetheorybasisofscreeningtheexcellentnitraginTheRhizobiumweredirectlyisolatedfromso

4、ybcannoduleTheinitialscreeningwasbasedonplantheight，dryweightofplantnumberofnodules，dryweightofnofulesandsomeotherindexesTheresultsshowedthatthebestvarietywasVt，followedbyV2，theoptimumstrainswereR1、R5and＆Thevarietiesands

5、trainswereusedinthefollowingpotexperimentThestrainsR1，％，‰andthecontrolRkwereinoculatedonthevarietiesVtandV2byspheroidizingtreatmentthentheinoculatedseedswereseparatelysownintheblacksoilandmeadowsoilIntheperiodofflourishf

6、lowersnitrogenaseactivityureide，symbioticnitrogenfixationefficiencyweredeterminedThequantityandqulityofsoybranweredeterminedduringtheharvest，Basedonvarianceanalysisandsignificancecomparison，thefollowingconclusionsweredro

7、wn：blacksiolrespondedbettertotheinoculumthanmeadowsoil；theyieldofV2washighersignificantlythanthatofV1，butsymbioticvalidityofinoculatedRhizobiumandV1wasmuchhigher；bothViandV2hadabroadspectruminoculum，andbettercompatibilit

8、ywhichwasprovedbytheeasierinfectionnodulationbymostofrhizobiaRlhadstrongestinfectingability，hi曲estcapabilityofsymbioticnitrogenfixationandbestmatchingwithhostMoreoverR1representedbettermatchingwithVIandstrongeradaptabili

9、tytoS2Italsoshowedbetterperformanceinnitmgenaseactivityureidecontent，dryweightofnodules，yieldandsomeotherindexesThebestmatchsofrhizobia，twovarietiesandtwotypesofsoilwere：RlVtSi、RIV2Sl、RIVlS2、RtV2S2Rhizobialegumesandsoilh

10、aveinfluenceonnodulationandnitrogenfixationAndsoiltypewastheprimaryfactoraffectingnodulationandnitrogenfixation，thenextwasinoculumIncrossingaction，thematchofsoybeanvarietyandrhizobiawasthemainfactorthataffectedsymbioticn