效應(yīng)T細(xì)胞過繼輸注子宮內(nèi)膜異位癥大鼠模型抑制異位病灶的實(shí)驗(yàn)研究.pdf

1、華中科技大學(xué)碩士學(xué)位論文效應(yīng)T細(xì)胞過繼輸注子宮內(nèi)膜異位癥大鼠模型抑制異位病灶的實(shí)驗(yàn)研究姓名：馮地路申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：婦產(chǎn)科學(xué)指導(dǎo)教師：汪宏波201005華中科技大學(xué)碩士學(xué)位論文華中科技大學(xué)碩士學(xué)位論文2ABSTRACTEndometriosisisconsideredoneofthemostfrequentdiseasesingynecologyaffecting1015%ofwomenintheirreproductiveage

2、.Themainclinicalfeaturesareinfertilityseveredysmenrheadyspareuniachronicpelvicpainabnmalmenstruation.Itisapparentthatthediseaserigouslyinterfereswiththepatients’qualityofwklife.Howeveranindepthunderstingofthecellularmole

3、cularmechanismsinvolvedinendometriosisisbeinguncoveredtheinadequateimmuneresponsesinthepelvicmicroenvironmentaresignificantlyinvolvedinthepathogenesisofendometriosis.Immunetoleranceplaysacentralroleintheimplantationdevel

4、opmentofexfoliatedendometrialcells.Indertousethepowerofimmunesurveillancemechanismpreventectopicendometrialcellstoevadetheimmunesystemthebonemarrowderiveddendriticcells(BMDCs)wereisolatedfrominbredlineratstopresenttheant

5、igenofratectopicendometrialcellsfintroducingproliferationofTlymphocyte.AftergenerationofexperimentalendometriosisbyectopicendometrialimplantationintheperitonealcavitytheeffectiveTcellswereinjectintotheabdominalcavityofra

6、ts(n=8).Resultshowedthattherewasasignificantdifferenceabouttheatrophyintheectopiclesionof3ratsinexperimentalgroupwiththenmalmodels(p0.05).wefoundoneratwithseverepelvicInflammatyreaction.TheseresultssuggestedthatInjection

7、effectiveTlymphocytesintoratswithendometriosiscouldevidentlyinhibitedtheectopiclesiondevelopment.CytotoxicTlymphocyte(CTL)couldbeinducedtocleartheectopicendometrialcellsbytheimmuneresponse.Changingthepelvicmicroenvironme