α-SMA和TGF-β1在骨肉瘤中的表達(dá)及意義.pdf

1、Objective: To study the significance and clinical value of expressions of a-smooth muscle actin (a-SMA) and transforming growth factor-beta (TGF-β1) in osteosarcomas. Methods: a-SMA and TGF-β1 were detected for the

2、specimens of 36 osteosarcomas and 20 osteochondromas and 21 osteosarcomas (before and after chemotherapy in the same patient ) by immunohistochemical Envision method and SPSS 11.5 software was used to analyze the signifi

3、cance and relationships of their expressions. Results: The positive rate of a-SMA was47.20 percent in osteosarcomas group and 60.70 percent in high-grade osteosarcomas group with a significant difference . The posit

4、ive rate of a-SMA was zero in osteochondromas group and in low-grade osteosarcomas group with a significant difference(χ2=13.56, P ＜ 0. 01) . The positive expression rate of TGF-β1 was41.70 ％ in osteosarcomas group and 5

5、1.30％ in high-grade osteosarcomas group with a significant difference . The positive expression rate of TGF-β1 was zero in osteochondromas group and in low-grade osteosarcomas group with a significant difference (χ2=11.3

6、8, P ＜ 0. 01). In osteosarcomas group, the expression of a-SMA was positively correlated with the expression of TGF-β1 ( rs = 0.546, P ＜ 0. 01).In osteosarcomas group with chemotherapy, the expression of α -SMA and TGF-

7、β1 was negatively correlated with the expression before chemotherapy. TGF- β1 (p＞0.05) ,α -SMA(p＞0.05). Fi sher' s Exact Test showed that patients with lung metastasis and recurrence had significant TGF-β land α-SMA expr

8、ession rates than those with disease-free survival. α-SMA (p＜0.05) TGF-βl(p＜0.05).in high-grade osteosarcomas group positive rates of a-SMA and TGF-β1were significant difference than those in low-grade osteosarcomas grou