南江黃羊生長軸主要基因多態(tài)性與生長性狀關(guān)系的研究.pdf

1、1108757分類號(hào)密級(jí)’jcI目差遺犬等博士學(xué)位論文單位代碼：10019學(xué)號(hào)：B030229南江黃羊生長軸主要基因多態(tài)性與生長性狀關(guān)系的研究AssociationofPolymorphismsinMajorGenesofGrowthHormoneAxiwithGrowthTraitsinNanjiangHuangGoats研究指導(dǎo)教生：瑟查蠶師：要壺迤數(shù)援合作指導(dǎo)教師：翌洼玲量』麴援申請學(xué)位門類級(jí)別：壅堂監(jiān)稱：麴物萱差曼飼鲞翌抖堂向：

2、廈芻動(dòng)物萱差院：動(dòng)物抖堂撞苤堂院2007年6月名方學(xué)業(yè)究在專研所AbstractThepresentstudywasconductedtofindthegeneticmarkersassociatedwiththegrowthtraitsinNanjiangHuanggoatsformarkerassistedselectionlinewithhighergrowthrateAtotalof405NanjiangHuanggoatswe

3、reusedtodetectthepolymorphismsofmajorgenesofgrowthaixsincludingGHRHgene，GHgene，GHRgene，IGFIgeneandIGFBP3genetoanalyzewhethertheycouldbecandidategenesormolecularmarkerstoimprovethegrowthtraitsinNanjiangHuanggoatsFiveexper

4、imentsweredesignedforthisobjectiveExperimentl：thepolymorphismsweredetectedatexon3，exon4andexon5ofGHgeneTheresultsofsequenceanalysisandmegalignshowedthat2SNPs(98G／A；108G／A)wasindentifedinexou3ofGHgene，4sNPs(27G／A；68C／A；13

5、6G／A；170C門_)ilIexon4ofGHgeneand3SNPs(36C／T；187C／T；202C／T)andlinsertion(241Cinsertion)inexert5ofGHgeneThecorrelationswereobservedbetweenthepolymorphismsofexon3andthetwomonthbodyweight，oneyearoldbodyweightTheleastsquaremea

6、nsoftwo—monthbodyweightandoneyearoldbodyweightofindividualswithgenotypeBBweresignificantlyhigherthanthatofgenotypeCCandAC儼o05)；Thecorrelationswereobservedbetweenthepolymorphismsofexon4andbirthweight，thetwomonthbodyweight

7、thesixmonthbodyweightIndividualswithgenotypeFFhadhigherbirthweighttwomonthbedyweightandsixmonthbodyweight妒o05)Themutationat136site(G／A)wasresponsibleforthethiscorrelation，whichindividualsofgenotypeGGhavehigherbirthweight

8、sixmonthbodyweightthantheseofgenotypeAG“k∞05)Thecorrelationswerenotfoundbetweenpolymorphismofexon5andbodyweightandbodyphysicalmeasuresExperiment2：EightexonssequmcewereclonedTenexonsand57flankingregionofGHRgeneweredetecte

9、dbytechniqueofPCRSSCPGrepeatandGTrepeatpolymorphismswereidentifiedin5“flankingregionofGHRgeneTheassociationanalysisrevealedthatpolymorphismshadasignificanteffectontwomonthweightsixmonthweightoneyearoldweightTheBDgenotype

10、hadlowertwomonthweightthantheothergenotypesrPO05)TheBDgenotypeandACgenotypohadlowersixmonthweightthantheAAgenotype，BBgenotypoandCCgenotype妒o05)ThefurtheranalysisresultshowedtheGrepeatwasresponsiblefortheassociationwithbe

11、dyweightExperiment3：Fourexonsand5“flankingregionofIGFIgeneweredetectedbytechniqueofPCRSSCPPolymorphismswasidentifiedatintron4ofIGF—IgeneThecorrelationsbetweengenotypesatthislocusandbirthweightoneyearoldweightweqefound(P0

12、05)TheCCganotypehadhigherbirthweight，oneyearoldweightthanCGgenotype(P005)Theeffectofgenotypeonbodymeasureswerenearlysignificant∞01)Experiment4：polymorphismofpartialgeneofGHRHwasinvestigatedbythetechniqueofPCRSSCPbutnopol

13、ymorphismwasrevealed砒partialofGHRHgeneinNanjiangHuanggoatsExperiment5：Threeexonsandintron2ofIGFBP3geneweredetectedbytechniqueofPCRSSCEButnopolymorphismswerefoundPolymorphismsofexon3andexon4ofOHgene，57flankingregionofGHRg