核因子kB在人年齡相關性白內障晶狀體前囊膜上皮細胞中表達的實驗研究.pdf

1、青島大學碩士學位論文核因子kB在人年齡相關性白內障晶狀體前囊膜上皮細胞中表達的實驗研究姓名：潘紹新申請學位級別：碩士專業(yè)：眼科學指導教師：趙桂秋20090531TheexpressionofnuclearfactorkappaBinepitheliumofhumananteriorlenscapsulewithagerelatedcataractABSTRACTObjectiveToexploretheroleofnucleartran

2、scriptfactorkappaB(NF—kB)ontheoccurrenceanddevelopmentoftheagerelatedcataractbyexaminingthedifferentexpressionofNFkBinepitheliumofhumananteriorlenscapsulebetweenthegroupwithage—relatedcataractandthecontrolgroupMethods60a

3、nteriorcapsuleswithagerelatedcataract(casegroup、Agroup)andlOnormallens(normalcontrolgroup、Bgroup)werecollectedbymicroscopeTheexpressionofNFKBP65proteinin30anteriorcapsuleswithage—relatedcataractsand5normallensweredetecte

4、drespectivelybyimmunohistochemistryandanalyzedbycolorgraphic—basedanalysissystem(OLYMPUSPROlSOES)TheexpressionofNFKBP65mRNAin30anteriorcapsuleswithage—relatedcataractsand5normallensweredetectedrespectivelybyreal—timepoly

5、merasechainreaction(realtimePCR)AndtheirdifferenceswerecomparedandtreatedusingIndependent—SamplesTtestinstatisticalpackagesSPSSl15ResultsTheyellowgranulescanbeseeninepithelialcellcytoplasmandnucleusofage—relatedcataracts

6、andnormallensLightyellowgranulesmainlybeseeninthecytoplasmofanteriorlenscapsulewithnormallensandbrowngranulesmainlybeSeeninthenucleusofanteriorlenscapsulewithagerelatedcataractComparedwithnormalcontrolgroup，theexpression

7、ofNF—rBp65proteinsignificantlyincreasedinc弱egroupsSignificanceisP005basedonIndependentSamplesTtest(t=62109)Comparedwithnormalcontrolgroup，theexpressionofNFrBp65mRNAsignificantlyincreasedincasegroupsSignificanceisP005base

8、donIndependentSamplesTtest0=129683)ConclusionNFkBmaybeakindoftranscriptfactortomaintaintheelementarymetabolicfunctioninlensepithelialcellsTheoverexpressionofNFrBmayplayanimportantroleontheoccurrenceanddevelopmentoftheage