含透明顫菌血紅蛋白基因重組菌的構(gòu)建及其對菌株發(fā)酵產(chǎn)1，3-丙二醇的影響.pdf

1、石河子大學碩士學位論文含透明顫菌血紅蛋白基因重組菌的構(gòu)建及其對菌株發(fā)酵產(chǎn)1，3丙二醇的影響姓名：裴海生申請學位級別：碩士專業(yè)：農(nóng)產(chǎn)品加工及貯藏工程指導教師：李春曹竹安20080601石河子大學碩士學位論文AbstractAbstractThe啦genecodingforVitreoscillaHemoglobinWaStransferredintowildtypestrainKoxytocaM5al，alsotheydhgenecodi

2、ngforformatedehydrogenaseand啦WeretransferredintoKoxytocaM5altogetherThentheinflunceofheterogeneousproteinsonthefementationof13一propanediolwasstudiedTheresultswereshownasfollowed：Wefoundthatdomesticationofstraincouldobvio

3、uslyenhancetheproductionof13propanedi01AfterdomesticationthecellgrowthWasbetterthanundomesticatedstrain，fermentationbrothdomesticationhadmoreinfluenceonglycerolconsumptionand13propanediolproductionaswellasoncellgrowthAlt

4、er45hours’fermentation，theyieldof13propanediolofglyceroldomesticatedstrainandfermentationbrothdomesticatedstrainwere658g‘1。1and736g‘l1，respectivelyComparedwiththewildtypestrain，theproductionof13propanediolwasenhanced412％

5、and5794％，respectivelyitstatedclearlythatfermentationbrothdomesticationcouldnotonlyenhancetheendurenceofsubstrateglycerol，butalsoenhancedendurenceofproductssuchas13propanediol，ethanol，lactateSofinallyitcouldenhancetheprod

6、uctivityof13propanedi01TheresultofshakingflaskfermentationunderdifferentsdissovedoxygenconditionsshowedthatdissovedoxygenWasamainfactorthatinfluncedthefermentationresults，500mlflaskfilledwith100mlmediumwouldbethebestdiss

7、ovedoxygencondition，andthefinalconcentrationof13propanediolof1129。1～WasgainedinthisconditionVitreoscillaHemoglobinandformatedehydrogenaseweresuccessfullyexpressedinKoxytocaM5al，CObondingexperimentandformatedehydrogenasee

8、nzymeactivityanalysisshowedthatthoseheterogeneousproteinshadfuctionalactivityRecombinentplasmidspDVandpDFVwereconstructedwhichcontainedparDEgene，comparedwithplasmidpVorpFVthestabilityofplasmidinrecombinantstrainscontaine

9、dparDEwereobviouslyenhancedContinuouspassage48hours，only233％、391％ofplasmidpDVandpDFVwerelost，whileabout696％、793％ofplasmidpVandpFVwerelostAnditshowedthattheFDHactivitywerealsolesslostbecauseofparDEgeneThe13PDproductionofr