TGFβ1-Smad2-3信號轉導通路在實驗性大鼠肝癌形成過程中的作用.pdf

1、復旦大學碩士學位論文TGFβ1Smad23信號轉導通路在實驗性大鼠肝癌形成過程中的作用姓名：胡志英申請學位級別：碩士專業(yè)：病理學指導教師：胡錫琪2002.5.22—————_——————————Ⅷ_●————————————’——一英文摘要TheefficacyofsignalingpathwayoftransforminggrowthfactorBl—Smad2／30nexperimentalrathepaticcarcinogene

2、sisAbstractIntroduction’transforminggrowthfactorB(TGFB)isanimportantcytokinethataffectsavarietyofbielogJcalprocesses，suchasgrowth，differentiation，development，andapoptosisofvariOUSce]1LypesandtissuesTGFBhasnegativeregulat

3、ionofcellpro]iferatioNbycausingcellstoaccumulateinG1phaseofthecellcycleHowevera10SSofgrowthinhibitoryresponsestoTGFBisoftenobservedinmanycancercellswhichissupposedtoberelatedtothedisruptionofTGFBsignalingSmad2and3areesse

4、ntialtotheintracellularsignalingpathwaysutilizedbymembersofthetransforminggrowthfactorB(TGFB)superfamilYTheycanbephosphorylatedbyTGFBreceptortypeIandresultnuclearconcentrationMutationinthissigna]ingsuchasTGFBreceptorSmad

5、2andSmad4havebeenreportedinseveralhumantumorsSonumerousresearchwerefocusedonthemasnewtumorsuppressorgenesObjectireInthisstudytherelationshipbetweentheefficacyofTGFBsignalingandhepatocarcinogensiswasinvestigatedby1“17si“t

6、uhybridizationandimmunohistochemistrytechniques，inordertounderstandthemechanismofhepatiocarolnogenesisMethodsWithinsituhybridizationandiNnunohistochemistrymethods，theexpressionanddistributionofTGFBlandSmad2and3incellswer

7、edetectedduringhepatocarcinogenesisoftheSDratinducedby3’MeDABResults1TGFB1transcriptsandtranslationweredetectedprimarilyinNonparenchymalcells(NPC)，suchashepaticstellatecells(HSCs)，endothelialcel1s，myofibroblasts(MFB)ands