血管緊張素轉(zhuǎn)換酶基因多態(tài)性與血管性癡呆發(fā)病關(guān)系的研究.pdf

1、青島大學(xué)碩士學(xué)位論文血管緊張素轉(zhuǎn)換酶基因多態(tài)性與血管性癡呆發(fā)病關(guān)系的研究姓名：劉宏申請學(xué)位級別：碩士專業(yè)：病原生物學(xué)指導(dǎo)教師：張文卿20030810英文摘要POLYMORPHISMOFANGl0TENSINCONVE—RTINGENZYMEGENEANDVASCULARDEMENTIAABSTRACTobjectiveToexploretherelationshipbetweenthepolymorphismoftheangiotens

2、inconvertingemyme(ACE)geneandvasculardementia(VD)Methods94easesofVD，60casesofessentialhypertensionand60casesofhealthycontrolswerechoseninthestudyPeripheralbloodWaStakenfromallthesubjects，andDNAwasextractedbyusingDNAextra

3、ctingkitAninsertion／deletionpolymorphismoftheACEgenewasanalyzedbypolymerasechainreaction(PER)Only490bpbandone—lectrophoresisrepresentedI／ItypeofACEgene，andonly190bpbandrepresentedD／DtypeofACEgene，andboth490bpand190bpband

4、srepresentedI／DtypeofACEgeneResults①Among94casesofVD，therewere33easesbelongingtODDgenotype(3511％)，45casesbelongingtoDIgenotype(4787％)，and16casesofIIgenotype(1702％)；ThefrequencyofDandIallelewas5904％and4096％respectivelyIn6

5、0casesofhealthycontrols。therewere10casesofDDgenotype(1667％)，28crisesofDIgenotype(4667％)，and22casesofIIgenotype(3666％)；ThefrequencyofDandIalleleinthisgroupwere4000％and6000％respectivelySoboththeDDgenotypeandthefrequencyofD

6、alleleofVDcasesweresignificantlyhigherthanthatofnormalhealthycon—trois(z2—10212，10657；PO，01，PO01)②In85casesofVDOCCurredaftercere—hralstroketherewere31casesofDDgenotype(3647“)，41casesofDIgenotype(4824％)，13casesofIIgenotyp

7、e(1529％)，andthefrequencyofDandIalleleswas6059％and3941％respectivelyInthe9casesofVDthatwerenotcausedbycerebralstroketherewere2casesofDDgenotype(2222％)，4casesofDIgenotype(4444％)，and3casesofIIgenotype(3334％)；andthefrequencyo

8、fDandIalleleswere4444蹦and5556％respectivelyTheseresultsshowedthatboththeDDgenotypeandthefrequencyofDalleleintheVDeasescausedbycerebralstrokewerehigherthanthatoftheVDcaseswhowerenotcausedbycerebralstrokeHoweverstatisticalt

9、estwasnotdonebecauseofthelimitedsubjects③Amongthe58easesofVDwithEH，therewere24casesbelongingtoDDgenotype(4138％)28easesbelongingtODIgenotype(4828％)，and6casesbe—longingtoIIgenotype(1034％)；andthefrequencyofDandIalleleswere6