樟樹優(yōu)良單株選擇與組培研究.pdf

1、福建農林大學碩士學位論文樟樹優(yōu)良單株選擇與組培研究姓名：彭東輝申請學位級別：碩士專業(yè)：森林培育指導教師：陳存及20040401OnSuperiorTreeSelectionandTissueCuItureofCinnamomumcamphoraAbstractThegeneticresourcesofCinnamomumcamphoradistributedovertheareaofwesternFujian(Nanping，Sanmi

2、ngandLongyan)，China，wereinvestigatedandanalysedIntermsofitschemicalcomposition，fourtypesofCinnamomumcamphorawereidentified，Linalool，CamphorCineolandTheui婦ownThemicroscopicstructuresofthe1eavescollectedfromtheformerthreet

3、ypesoftreeswerefoundtobedifferentundertheopticalandelectronmicroscopesWithreferencetothestandardoflinaloolproductsintheintemationalmarket，theselectivecritefionforsuperiortreeswasdeterminedthroughstatisticalanalysesandcon

4、sequentlyfivesuperiortreeswereselectedfortheexperimentThefactorsinfluencingtissuecultureoftheselectedsuperiortreeswerestudied，indicatingthattheoptimumexplantsarethetoptenderstemsunderthethirdnodes，andtheoptimuminitialmed

5、iumisMSBAlOIBAO1(orNAA01)Thepropagationcultureexperimentsrevealed：(1)Thesimultaneousoccurrenceofthetwopropagationways，shootingradiallyfromthebaseofthestemsandtheaxillaryshootsofthemainstems，isbeneficialforelevatingthepro

6、pagationrate；(2)TheoptimummediumselectedfromtheexperimentsisMSBA30IBA02，withtheaveragepropagationcoefficientreaching61afterbeingculturedinthemediumfor45days；(3)Whenagarconcen訂ationandpHvaluesofthemediumwereadjustedto60g／

7、1and60，respectivelytheseverevitrificationinsubculturepropagationprocesswaseffectivelyalleviated；(4)ByaddingantioxidantVCtothemedium，usingsuitableexplantmaterials，andshorteningsubculturecycle，brownificationcallbemitigated