17402.菲律賓蛤仔(ruditapesphilippinarum)抑菌酶解物的制備與作用機理研究

1、要本文以菲律賓蛤仔為研究對象，篩選有抑菌活性的酶解物，i：優(yōu)化其酶解：【：藝；對酶解物進行初步的分級純化，爿二研究j11】茵活。肚影響l列索、探討其作JIJ4JLSq!。研究結(jié)果如下：l、菲律賓蛤仔胃蛋白酶酶解產(chǎn)物時命黃色衙苘球蔭、人腸桿1菊、枯草芽孢桿菌、釀酒酵母和副溶血弧菌均有一定的抑菌活性，其中對會’黃色葡萄球藺和枯草芽孢桿菌的抑菌活性最強，對17U聯(lián)微球菌則無明屆的#lJi輔活性。最優(yōu)酶解?。核嚄l件為：酶解時fnj2h，溫度35

2、℃，pH2，加酶!gRJ3000U，料水比l：4。2、菲律賓蛤仔抑倚活性酶解物1|=l對分了質(zhì)鍵：卜嬰分們祚5000D以下。川對分子質(zhì)量4q：1000Dn勺紕分占2731％，I：nf分jJ乞質(zhì)邋住1000～3000D之川的組分占4219％，相對分予質(zhì)量在3000～5000D之l’HJ的組分占2849％，村l對分子質(zhì)量大于5000D的組分僅占191％。非律賓蛤仔抑菌活性酶解物經(jīng)超濾杯分級后得到3個組分：組分I(3KD10000Donlya

3、ccountedfor191％TheantibacterialhydrolysatesofRuditapesphilippinarurnwasclassificatedintothreecomponentsbyultrafiltrationcup：componentI(3KDMr5KD)，component11(IKDMr3KD)，componentIII(Mr1KD)1nhecomponentsIIhadthestrongestant

4、ibacterialactivityagainstStaph)7Iococcusaureus，followedbycomponentIII(Mr1KD)Theantibacterialactivityofcomponentsl(3KDMr5KD)wastheweakestHightemperature，longstoragetimeandstrongalkalineenvironmentwillreducetheantibacteria

5、lactivityofhydrolysatewithpepsinagainstStaphylococcusaureus；UVtreatmenthasnoeffectontheantibacterialactivity3TheantibacterialhydrolysateofRuditapesphilippinarumchangedthecolonymorphologyofSfnphylococcusallrPZl$andBacillu

6、ssubtilisandinhibitedthegrowthoftwokindsofbacteriainthelogarithmicphaseTheresultsshowedthatthecontentofprotein，solublesugarandUVabsorbingcompoundsandtheconductivityintheculturemediumoftwokindsofbacteriaincreasedItcouldbe

7、alsodetectedtheactivityofalkalinephosphataseandbetagalactosidaseintheculturesolutionandphosphorusmetabolismofthetwokindsofbacteriawereseriouslyhampered111eresultsofscanningelectronmicroscoperevealedthatitemergedthephenom

8、enonofagglomerationwiththeextensionofresponsetimeintheculturesolutionofSeaphvlococcusaureusandthecellshapebecameirregularalargenumberofcellsstickedtogetherafterwardsintracellularmaterialspilled，theboundariesbetweencellsc