19835.水稻響應(yīng)鹽脅迫和低溫脅迫的蛋白質(zhì)組研究

1、分類號UDC密級編號中國科學(xué)院研究生院博士學(xué)位論文工857Dl盛播照廑墼赴追塑低漫毖墊鰉置自廈塑受塞芒題壬指導(dǎo)教師遂童縫登盔雖菱絲籠受盔雖一一蟲圜撾堂瞳土篷生僉越鱟殛塞睦燕塑生堡生盎班基匿申請學(xué)位級別絲學(xué)科專業(yè)名穆一一撞塑堂論文提交日期2逝生疊屋論文答辯日期2壁鰱璽墨且培養(yǎng)單位主國魁翌陵圭篷生盒登鱟瑟塞毖蕊塑生毽生盔亟塞逝學(xué)位授予單位一一蟲曩盈堂陵盈塞壘黷答辯委員會主席摘要ABSTRAcTShunPingYanfPlantScience

2、s)DirectedbyProfessorZhangChengTangandProfessorWeiAiSuProteomicsstudyisplayingartincreasinglyimportantroleinthefunctionalgenomicsera。TwodimensionalelectrophoresisisoneofthekeytechniquesinproteomicsstudiesRice(Oryzasativa

3、L)isnotonlythemostimportantcropworldwide，butalsothemodelplantforthemonoeotsWeoptimizedthericeproteinextractionmethodandtheelectrophoresisconditions，andobtainedthehighresolutionandreproduciblegelmapsAtleast1150，1000，1050p

4、roteinspotsweredetectedonthegelsofroot，leafandsuspensioncell，respectivelyThecorrelationcoefficientbetweenreplicatesvariedfromO87toO94SaltstressisoneofthemajorabioticStressesaffectingtheproductivityofcropsallovertheworldI

5、twasestimatedthatabout20％oftheworldlandand50％ofallirrigatedlandwereaffectedbysalinityTherewere34upregulatedand20downregulatedrootproteinsinthethreeweekoldriceseedlings(cvNipponbare)treatedwith150mMNaClfor24，48and72hMasss

6、pectrometryanalysishelpedtoidentify12spotsrepresenting10differentproteinsThreespotswereidentifiedasthessmeproteinenolaseⅥmilefourofthemwerepreviouslyconfirmedassaltstressresponsiveproteins，6werenovelones，ieUDP—glucosepyr

7、ophosphorylase，cytochromeCoxidasesubunit6b1，glutaminesynthetaserootisozyme，putativenascentpolypeptideassociatedcomplexalphachain，putativesplicingfactor—likeproteinandputativeaetin—bindingproteinTheseproteinsareinvolvedin

8、carbohydrate，nitrogenandenergymetabolism，reactiveoxygenspeciesscavenging，mRNAandproteinprocessing，andcytoskelctonstabilityLowtemperaturestressisanothermajorabioticstresslimitingtheproductivityandthegeographicaldistributi

9、onofmanyimportantcropsThreeweekoldriceseedlingstreatedat60Cfor6or24hshowedstressphenotypesofrollingleaves，increasedrelativeelectrolyteleakageanddecreasednetphotosyntheticrateAmongmorethan1000leaftotalproteinsdetectedontw