60325.海洋赤潮生物塔馬亞歷山大藻的生理生態(tài)特征及其同廈門海域幾種細(xì)菌的生態(tài)關(guān)系

1、廈門大學(xué)碩士學(xué)位論文海洋赤潮生物塔馬亞歷山大藻的生理生態(tài)特征及其同廈門海域幾種細(xì)菌的生態(tài)關(guān)系姓名：王艷麗申請學(xué)位級別：碩士專業(yè)：植物學(xué)指導(dǎo)教師：連玉武鄭天凌19990801StudyonthephysiologicalandecologicalpropertiesofAlexandri幽tenmreaseandtheecologicalrelationshipbetweenthealgaandseveralkindsofbacteria

2、isolatedfromXiamenHarborAbstraet：ThisdissertationfocusesonthephysiologicalandecologicalpropertiesofAlexaadriumt勰arensef(Lebour)Balcch，1985]，akindofredtide—formingalgaandtheecological，一aelationshipbetweenthealgaandthreeki

3、ndsofbacteriaisolatedfromXiamenHarborAsasub—programofthe‘‘TheDynamicsandthePreventionBaseoftheRedTideOccurredInSouth—China’stypicalaquiculturearea’’supposedbyNSF’China，thestudyismainlycarriedinthelaboratoryThefollowingar

4、cthemainresults：1A。tamarenseisaprimordialunieellulardinoflagellatethat、likeProtocentrum所icartshasnokaryothecaarounditskaryon，AtamarelqseCallgrowthwelIandkeepsomeofitspeculiaritywhenculturedunderthelaboratoryconditionItsg

5、rowthcu／vcisassameaswhichofthegeneralmicroorganismBacteriaarenotfoundinthepurelyculturedAtamarensecell2ThreestrainsofBacteriaareisolatedfromthesedimentsofXiamenHarborAfterthemorphologicalexanfinationandthemoleculebiologi

6、calstudyitcartbeidentitledthatS5probablyisnewspecieofSphingomonas，S7isBacillusmegateriumandS10isBacillushalmapalus3TherelationshipbetweenthreestrainsofbacteriaSs，S“StoandthealgaAtamarenseisstudiedbyaddingthebacteria’sfil

7、trateintothealgacultureseparatelyinthelagphaseandexponentialphaseofthealgagrowthThethreestrainshavedifferenteffjctHigherconcentration(2x10”eell／mL)filtrateofbacteriaSEandSlnCallmarkedlyinhibitthealga’sgrowthandproliferat

8、ionS10hasthes廿ongerinhibitingeffectthanS5W11ilemiddleconcentration(2109cell／ⅢL)filtrateofSEandSlncallmarkedlyimprovethegrowthandproliferationofthealgaS1。hasthes打ongerpromotionthanSstothealga’sgrowthTheinhibitionandpromot

9、ionarebothmoreremarkableinthealga’slagphasethanexponentialphaseThelowerconcentration(2x108eel1／mL)filtrateofS5andSlnhasDOevidenteffectonthealga’sgrowthBacteriuInS，hasnoclearinhibitionorpromotiontothealga4Theactivitvofext

10、racellularenzyme13GIcAislowattheprophasebutrisequicldyattheanaphaseofthealgagrowthinpureeultureThe[3GlcAoftheculturewatercanbepromotedremarkablybyaddingbacteriaS1nButbacteriurnS，hasnodistincteffectontheactivityofextracel

11、lularenzyme5，Itcarlbeseenthroughtheelectronmicroscopethatthebacteriacaninvadedirectlyintothecytoplasmofthealgacellwhenhigherconcentrationbacteria(S5andSIo)solutionaddedinthealgacultureThebacteriamainlylieintheSUCCUSandth

12、ancanbetransferredintotheorganelleThebacteriacaninhibitthealgacell’sdivisionsoastoinhibititsproliferationLargenumbersofbacteriathoseinvadedthealgaeellGalldoeithermorphologicorstructuralharmtothecellandcarlspeeduptheaging