31171.林間松墨天牛的誘捕及其所攜帶松材線蟲的快速檢測和疑似病木的早期診斷

1、廈門大學(xué)碩士學(xué)位論文林間松墨天牛的誘捕及其所攜帶松材線蟲的快速檢測和疑似病木的早期診斷姓名：張阜嘉申請學(xué)位級(jí)別：碩士專業(yè)：動(dòng)物學(xué)指導(dǎo)教師：潘滄桑20090501廈門大學(xué)2009年碩士學(xué)位論文AbstractPineWiltDiseaseisal【i11dofdestructivepinetreediseasewhichisseverelythreateningforestssafetyinmanycountriesandhascause

2、dgreateconomiclosseveryyearaswellasimponderableecologicaldamageItscausativeagent，pinewoodnematode(Bursaphelenchusxylophilus，PWN)，isaquarantineorganismatthetopofthelistofthepathogenicspeciesinmanycountriesandalsolistedonA

3、2inChinaThepinewoodnematodeistransmittedbyMonochamusalternatus，whicharenotonlytheprimaryvectorofBxylophilusinChinabutalsotheimportantstemborersofnativeconiferousforestsThesebeetlevectorstransmitthePWNtonewhosttreesviathe

4、feedingwoundsandtheovipositionwoundsSocontrollingthepopulationofMonochamusalternatuscouldnotonlyrelievedirectdamagesbutpreventBxylophilusspreadingHoweveritisdifficulttodifferentiateBxylophilusfromthecloselyrelatedspecies

5、BmucronatuswhichisnotpathogenictopinetreesundermicroscopefortheirmorphologicalsimilaritywhichmakesthequarantineandpreventionverydifficulttoperformInthisstudywefocusedonMalternatus，BxylophilusandPWN—infestedpinewoodApplyi

6、ngtherapiddetectionandearlydiagnosistechniquesofBxylophilus，wemonitorthePWNintheadultbodyofMalternatusaccuratelyandtimelyAnumberofMalternatusinsectswereinducedwiththeinsectcatchersintheepidemicregionfromApriltoSeptemberi

7、n2008Basedonreal—timePCRassayinvestigatedthenematodescarryingsituationoftheMalternatusOurstudycalleffectivelydetectPWNisolatedfromtrappedMalternatusearlyandhelptowipeouttheinfectantandpreventthefurtherspreadofPWNInthisst

8、udyweappliedtheearlydiagnosistubetothePWN—infestedpinewoodfromepidemicregionsandtheexperimentsindicatedthattheearlydiagnosistubehaseffectiveBxylophilusinducingcapacityWefurthertesttheefficiencyoftheearlydiagnosistubeandt