吸水過程中發(fā)菜光合活性及相關(guān)轉(zhuǎn)錄本的變化

1、摘要發(fā)菜(發(fā)狀念珠藻，NostocflagellifmeB而PSII電子傳遞活性的恢復(fù)比較緩慢，在吸水的第三階段逐漸恢復(fù)。于燥保存8年的樣品在回復(fù)吸水24h依然檢測不到葉綠素?zé)晒猓矙z測不到其PSII電子傳遞活性，而干燥保存2年的樣品PSII電子傳遞活性在回復(fù)吸水24h基本恢復(fù)。利用WATERPAM測定回復(fù)吸水過程中千燥保存2年發(fā)菜的快速光響應(yīng)曲線。隨著回復(fù)吸水時(shí)間的增加，藻細(xì)胞的光適應(yīng)系數(shù)Ex呈上升趨勢，這表明隨著回復(fù)吸水時(shí)間的增加藻

2、細(xì)胞能耐受的光照強(qiáng)度越高:最大電子傳遞速率ETRma二和表觀光合效率a也呈L升趨勢，這表明在回復(fù)吸水過程中光合電一子傳遞鏈的恢復(fù)情況。通過比對其他藻株的光合作用相關(guān)基因(psbAl、psbA2和psaA)的保守區(qū)域，electrontransptactivitycouldnotbedetectedinsamplesdriedf8years.Duringtheprocessofrehydrationtherapidlightcurveof

3、N.flagellifmedriedf2yearswasassayedwithWATERPAM.TheEkvaluesincreasedduringrehydrationindicatingthatN.flagellifmecouldutilizehighlightmeefficiently.TheETRmaxαalsoincreasedinthisprocessindicatingtherecoveryofitselectrontra

4、nsptactivityduringrehydration.Thentheexpressionofpossiblephotosyntheticgenes(rbcLrbcXpsbAlpsbA2)inN.flagellifmewasinvestigatedduringrehydration.TherbcLrbcXpsbAlpsbA2transofN.flagellifmedriedf2yearswereupregulatedduringth

5、erewettingprocessbuttheirlevelswerelessinsamplesdriedf8years.Within15minutesrehydrationtheabundanceofpsbA1genereached36%offullyrecoveredsamplesbuttheabundanceofpsbA2genewasverylowinthesecondphaseofrehydration.Thisindicat

6、edthatRubiscoD1proteininPSIIreactioncenterweredamagedduringwaterlossdrystagetheyshouldberepairedduringrehydration.ThedifferenceintheexpressionofpsbAlpsbA2suggestedthattherevoveryofphotosyntheticmachinemaybeacomplexproces

7、sduringrehydrationalthoughitisnotclearlyknownnow.ThelongerstageindrynesstherecoveryofphotosyntheticgenesexpressionneededmetimeindicatingthatlongtimestagemightcauseseriouslydamageonDNAtranionrelatedproteinsthismayberelate

8、dwithROSproduction.Keywds:NostocflagellifmrehydrationphotosynthesisphotosyntheticelectrontransptrapidlightcurveRealtimeRTPCRphotosyntheticgene目錄目錄摘要........................................................................

9、.........................................................IAbstract................................................................................................................................lI第一章發(fā)菜的研究進(jìn)展..............

10、..................................................................................l1.概述................................................................................................................................11.1地

11、理分布生態(tài)特征.............................................................................................................11.2形態(tài)特征................................................................................................

12、..............31.3生長繁殖方式....................................................................................................................41.4化學(xué)組成........................................................................

13、...............................52.生理生化特性.....................................................................................................................62.1水分生理.......................................................

14、.................................................................62.2光合生理..............................................................................................................92.3固氮作用.............................

15、.......................................................................................113.人工養(yǎng)致............................................................................................................................1