《pet質(zhì)粒圖譜大全》

1、Novagen?DERING8005267319?TECHNICALSUPPT8002070144AGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGAGACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG

2、AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAMetAlaSerMetThrGlyGlyGlnGlnMetGlyArgGlySerGlyCysEndpET3b...GGTCGGGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAA...GlyArgAspProAlaAlaAsnLysAlaArgLysGluAlaGlu

3、LeuAlaAlaAlaThrAlaGluGlnEnd...TACCATGGCTAGC...pET3cd...GGTCGGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAMetAlaSer......GlyArgIleArgLeuLeuThrLysProGluArgLysLeuSerTrpLeuLeuProProLeuSerAsnAsnEndC

4、CCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGNcoIpET3dNdeIT7?TagT7terminatBamHIBpu1102IT7terminatprimer#693373pET3arbsBglIIXbaIT7promoterprimer#693483T7promoterThepET3advectscarryanNterminalT7?TagsequenceBamHIcloningsite.Thesev

5、ectsaretheprecursstomanypETfamilyvectsthepET23ad()seriescrespondstopET3adbutincpatesseveraladditionalfeatures.Uniquesitesareshownonthecirclemap.NotethatthesequenceisnumberedbythepBR322conventionsotheT7expressionregionisr

6、eversedonthecircularmap.ThecloningexpressionregionofthecodingstrtranscribedbyT7RNApolymeraseisshownbelow.pET3asequencelmarksT7promoter615631T7tranionstart614T7?Tagcodingsequence519551T7terminat404450pBR322igin2814blacodi

7、ngsequence35754432ThemapsfpET3bpET3cpET3darethesameaspET3a(shown)withthefollowingexceptions:pET3bisa4639bpplassubtract1bpfromeachsitebeyondBamHIat510.pET3cisa4638bpplassubtract2bpfromeachsitebeyondBamHIat510.pET3disa4637

8、bpplastheBamHIsiteisinthesamereadingframeasinpET3c.AnNcoIsiteissubstitutedftheNdeIsitewitha1bpdeletionatposition550ofpET3c.AsaresultNcoIcutspET3dat546.Ftherestofthesitessubtract3bpfromeachsitebeyondposition551inpET3a.Nde

9、IdoesnotcutpET3d.pET3adcloningexpressionregionpET3adVectsTB0261298pET3a(4640bp)i(2814)Ap(35754432)ClaI(24)HindIII(29)Bpu1102I(458)BamHI(510)NdeI(550)XbaI(588)BglII(646)SgrAI(687)SphI(843)EcoNI(903)SalI(928)PshAI(993)EagI

10、(1216)NruI(1251)ApaBI(1329)BspMI(1331)BsmI(1636)AvaI(1702)MscI(1723)Bpu10I(1858)BscGI(1912)PvuII(2343)BsmBI(2393)Tth111I(2497)BsaAI(2504)Bst1107I(2523)SapI(2636)AflIII(2752)BspLU11I(2752)AlwNI(3168)HgiEII(3338)PstI(3890)

11、PvuI(4015)ScaI(4125)SspI(4449)AatII(4567)EcI(4638)ApoI(4638)Eam1105I(3645)–Cat.No.pET3aDNA694183pET3bDNA694193pET3cDNA694203pET3dDNA694213Novagen?DERING8005267319?TECHNICALSUPPT8002070144AGATCTCGATCCCGCGAAATTAATACGACTCAC

12、TATAGGGAGACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGATCCGAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATMetAlaSerMetThrGlyGlyGlnGlnMetGlyArgGlySerGluPhe

13、LeuLysThrLysGlyProArgAspThrProIlePheIleGlyEndpET5b...GGTCGGGATCCGAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAAT...GlyArgAspProAsnSerEndpET5c...GGTCGGATCCGAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAA

14、TGTCATGATAAT...GlyArgIleArgIleLeuGluAspGluArgAlaSerEndNdeIEcIT7?TagBamHINheIpET5arbsBglIIXbaIT7promoterprimer#693483T7promoterpBR322EcIclockwiseprimeri(2308)Ap(30693926)BamHI(4)NheI(37)NdeI(44)XbaI(82)BglII(140)SgrAI(181

15、)SphI(337)EcoNI(397)SalI(422)PshAI(487)EagI(710)NruI(745)ApaBI(823)BspMI(825)BsmI(1130)StyI(1140)AvaI(1196)MscI(1217)Bpu10I(1352)BsgI(1406)BspEI(1435)PvuII(1837)BsmBI(1887)Tth111I(1991)BsaAI(1998)Bst1107I(2017)SapI(2130)

16、AflIII(2246)BspLU11I(2246)AlwNI(2662)HgiEII(2832)PstI(3384)PvuI(3509)ScaI(3619)SspI(3943)AatII(4061)EcI(4132)ApoI(4132)pET5accloningexpressionregionTB0291298ThepET5acvectscarryanNterminalT7?TagsequenceBamHIEcIcloningsite

17、s.TheT7terminatpresentonmanyotherpETvectsismissinginthepET5acseriestherefeβlactamaseisgenerallyproducedinlargeamountsinpET5recombinantsinducedfT7RNApolymeraseexpression.ThepET23ad()seriescrespondstopET5acbutincpatessever

18、aladditionalfeaturesincludingtheT7terminat(Cat.No.693373).Uniquesitesareshownonthecirclemap.NotethatthesequenceisnumberedbythepBR322conventionsotheT7expressionregionisreversedonthecircularmap.Thecloningexpressionregionof

19、thecodingstrtranscribedbyT7RNApolymeraseisshownbelow.pET5a()sequencelmarksT7promoter109125T7tranionstart108pBR322igin2308blacodingsequence30693926ThemapsfpET5bpET5carethesameaspET5a(shown)withthefollowingexceptions:pET5b

20、isa4133bpplassubtract1bpfromeachsitebeyondBamHIat4.pET5cisa4132bpplassubtract2bpfromeachsitebeyondBamHIat4.pET5acVectspET5a(4134bp)Eam1105I(3139)–Cat.No.pET5aDNA694263pET5bDNA694273pET5cDNA694283本本資資源源由由生生物物秀秀搜搜集集整整理理并并提

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