分子印跡聚合物的制備及其在色譜分離中的應用.pdf

1、東南大學博士學位論文分子印跡聚合物的制備及其在色譜分離中的應用姓名：戎非申請學位級別：博士專業(yè)：生物醫(yī)學工程指導教師：袁春偉付德剛20071007AbstractMolecularlyimprintedpolymers(MIPs)annovelpolymericmaterialswithartificialreceptorlikebindingsiteforspecifictemplatesOnaccountoftheirhighrec

2、ognitionabilitystablemechanicandchemicalcharacteristics，MIPshavefavorableprospectina、“derangeofapplicationssuch笛chromatographic∞p刪ioTIsolidphaseextractionSel∞Orscomponents，mimice11zymecatalystsandmemberseparationInthisdi

3、ssertationapplicationsofMIPsinseparationofqulnolonesandthinlayerchromatographywereinvestigatedthehn朗fac廿Onsbetwccrltemplateandvariousfunctionalmonomerswerestudiedandnovelsurfaceim兩ntedmicrosphereswithbothsatisfyingkineti

4、cpropertiesanddesiredparticlemorphologiesWCIIgsynthesizedThemainworI【softhisdissertationa他presentedasfollows：MlPswithhighselectivityforLpazufloxacinwerepreparedEffectsofmonomerarlultraviolet(Uv)sp∞仉lnlofLpazufloxacinwere

5、investigatedtodeterminethebindingconstantandstoichiometricratiobetweenthetemplateandmonomerThebindingcharacteristicofMIPswasstudiedthroughbindingexperimentsandScatohardanalysesTheMIPsWasusedasstationaryphaseofhighperform

6、anceliquidchromatography(HPLC)toevaluateitsrecognitionabilityandseparationefficiencyAndtheinfluenceofpHvalue，ratioofbufferandorganicsolvontinthemobilephase，concentrationofsampleontheresolutionofMIPWerediscussedThinlayerc

7、hromatography(TLC)usingMIPsaschj_ralstationaryphases(CSPs)wasappliedtothedeterminationofen∞tiomarsofmandelicacidanditsderivativesInpreparationofMIPsLmandelicacidL2chloromandelicacidandL4chloromandelicacidwereemployed∞tem

8、platesrespectivelyTheinteractionDet3Ⅳecntemplateandmonomerwe”researchedbyUVandinfrared(IR)spectrumThecavitiesandmorphologiesofMIPs笛wella5blankpolymerWeqlecharacterizedbyscanningelectricmicroscopy(SEM)andliquidnitroganabs

9、orptionFurthermore，theCSPswe∞edoptedtesolvetheracematesoftemplatederivativestherelationshipbetweenthechemicalstructureoftheanalytesandchiralrecognitionoftheCSPswerealsoinvestigaiedMIPswithSnaproxenastemplateWeql℃prepared

10、usingdifferentfunctionalmonomersThe眥戌IctiOnsbe竹veenSnaproxenandfunctionalmonomaswe佗studiedbyUVandIRspectrumThepossiblecomplexesformedbySnapmxenanddifferentmonomerswemsimulatedbyHypcrehemtocalculatethebindingenergiesofsel

11、fassemblyThebindingcapabilityofMlPswasdiscussedthroughbindingexperimentsandScabbardanalyses，andtheretentionandresolutionabilitiesofMIPschiralstationaryphasewerecomparedbyMlPsbasedTLCTherelationshipsbetweentheresultsofmol