簡介：河北經貿大學碩士學位論文亞洲新興國家對外貿易與經濟增長關系的實證研究姓名李承斌申請學位級別碩士專業(yè)國際貿易學指導教師王勝利20070501亞洲新興國家對外貿易與經濟增長關系的實證研究IIABSTRACTSTUDYONFOREIGNTRADEANDECONOMICGROWTHISONEOFFOCUSINTHETHEORYOFTRADEANDDEVELOPMENTECONOMICSSCHOLARS’EXTENSIVEATTENTIONISCAUSEDBYFOREIGNTRADEPROMOTESECONOMICGROWTH,BUTCANNOTARRIVEATANAGREEMENTSINCETHEREFORMANDOPENINGUP,CHINAHASIMPLEMENTEDEXPORTORIENTEDDEVELOPMENTSTRATEGY,FOREIGNTRADEISDEVELOPINGFASTLY,WHICHPROMOTESECONOMICDEVELOPMENTSTUDYONEFFECTTHATCOUNTRIESFOREIGNTRADETOWARDSECONOMICDEVELOPMENTWILLBESIGNIFICATIVEFORCHINATODEVELOPECONOMYTHOUGHFOREIGNTRADEUNDERECONOMICGLOBALIZATIONUSINGJOHANSENCOINTEGRATIONTESTANDGRANGERCAUSALITYTEST,THISPAPERTRIESTODEMONSTRATEASIANEMERGINGCOUNTRIES’RELATIONSHIPBETWEENFOREIGNTRADEANDECONOMICGROWTHSTUDYINGFROM1980TO2007,USINGDATASFROMCHINA,SOUTHKOREAANDSINGAPOREETCEIGHTASIANEMERGINGCOUNTRY,EMPIRICALANALYZEEXPORTS,IMPORTSANDGDPCHINA,THAILAND,PHILIPPINES,MALAYSIAANDSINGAPORESGDPHAVEALONGTERMSTABLECOINTEGRATIONRELATIONSHIPBETWEENIMPORT,EXPORTANDGDPEIGHTCOUNTRIES’SEXPORTPROMOTEECONOMICDEVELOPMENTANDCHINAHASASIGNIFICANTINFLUENCEBECAUSEOFFINANCIALCRISISANDOTHERNEGATIVEFACTORS,CHINANEEDSTOCONTINUEDEVELOPFOREIGNTRADE,MAINTAININGRAPIDANDSTABLEECONOMICGROWTHTHISINNOVATIONLIESINTHREEASPECTSTHERESEARCHANGLE,THEREARELOTSOFEMPIRICALSTUDYONFOREIGNTRADEANDECONOMICGROWTH,BUTFEWABOUTSEVERALCOUNTRIESAFTER1980THESCOPEOFRESEARCH,THISPAPERISWRITINGEIGHTASIANEMERGINGCOUNTRIES,INCLUDINGCHINATHECONTENTANDECONOMETRICANALYSISMETHODSISRICH,INCLUDINGCOMPREHENSIVEINVESTIGATIONOFCOINTEGRATIONRELATIONSHIPANDERRORCORRECTIONMODELKEYWORDSASIANEMERGINGCOUNTRY；FOREIGNTRADE；ECONOMICGROWTH；COINTEGRATION

簡介：廈門大學碩士學位論文中國吸收FDI對亞洲國家或地區(qū)的擠出效應分析姓名林宏申請學位級別碩士專業(yè)國際貿易學指導教師陳濤20080501ABSTRACTITISANECONOMICCERTAINTYTHATTHECOUNTRY’SDEVELOPMENTHASACLOSERELATIONSHIPTOITSFDIINFLOWRAPIDLYDEVELOPING，FOREIGNDIRECTINVESTMENTHASSURPASSEDINTERNATIONALTRADETOBECOMEPERHAPSTHEMOSTIMPORTANTMEANSOFINTERNATIONALECONOMICCONNECTION，ASWELLASFUNCTIONINGASTHEMAINPARTOFFOREIGNCAPITALINDEVELOPINGCOUNTRIESTOCOMPETEFORTHESESCARCERESOURCES，COUNTRIESAROUNDTHEWORLDHAVELOWEREDTHEIRTHRESHOLDOFINWARDFDIDUETOTHISPHENOMENON，ECONOMISTSANDROSEARCHINSTITUTIONSHAVEBEGUNTOSTUDYTHEEFFECTSOFFDIBASEDONTHEINTRODUCTIONOFFDITHEORIESANDTHEIRDEVELOPMENTS，ASWELLASTHEFINDINGSOFFDIMODELSCONSTRUCTEDBYECONOMISTSBOTHINANDOUTSIDEOFCHINA，THISTHESIS’SFOCUSISONTHECROWDOUTEFFECTOFCHINA’SFDIINFLOWTOITSASIANCOMPETITORSSINCECHINABEGANITSREFORMATIONANDOPENDOORPOLICIESTHIRTYYEARSAGO，CHINA’SFDIINFLOWHASINCREASEDINCREMENTALLYEACHYEARNOTABLYIN2003。CHINA’SFDIINFLOWSURPASSEDTHATOFTHEUNITEDSTATES，FORTHEFIRSTTIMEHAVINGTHELARGESTHDIINFLOWINTHEWORLDCHINA’S同DIINFLOWHASHADASUBSTANTIALEFFECTONTHECHINESEECONOMYASWELLASPROMOTINGMARKETIZATIONWEOFTENFOCUSONTHEAMOUNTOFFDIINFLOWTOAPARTICULARCOUNTRY，HOWEVERWERARELYNOTEWHETHERTHISINCREASEHASNEGATIVEEFFECTSINRELATIONTOTHATOFOTHERCOUNTRIESFORTHISREASON，THISTHESISFIRSTLYANALYZESTHEHISTORYANDPRESENTSTATUSOFCHINAANDOTHERASIACOUNTRIES’FDIINFLOWS，ADDITIONALLYDISPLAYINGTHERESEARCHOFTHEEFFECTSOFFDIONOTHERASIANCOUNTRIESWHENMULTINATIONALCORPORATIONSMAKEFDIDECISIONS，THEINSTITUTIONALENVIRONMENTOFTHEHOSTCOUNTRYISALSOCONSIDEREDBASEDONTHISOBSERVATION，THISTHESISALSODEMONSTRATESACROSSCOUNTRYCOMPARISONBETWEENCHINAANDOTHERASIANCOUNTRIES’INSTITUTIONALINDEXESTOFINDWHETHERORNOTTHEDEFECTOFCHINA’SINSTITUTIONALENVIRONMENTHASAFFECTEDITSFDIINFLOWAFTERREVIEWINGEXISTINGLITERATURE，WEHAVEFOUNDTHATTHEREWASLITTLERESEARCHONTHECROWDOUTEFFECTOFCHINA’SFDIINFLOWONITSASIANCOMPETITORSASARESULT，TOCOMPLIMENT

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簡介：南開大學學位論文版權使用授權書毒7≤29本人完全了解南開大學關于收集、保存、使用學位論文的規(guī)定，同意如下各項內容按照學校要求提交學位論文的印刷本和電子版本；學校有權保存學位論文的印刷本和電子版，并采用影印、縮印、掃描、數字化或其它手段保存論文；學校有權提供目錄檢索以及提供本學位論文全文或者部分的閱覽服務；學校有權按有關規(guī)定向國家有關部門或者機構送交論文的復印件和電子版；在不以贏利為目的的前提下，學?？梢赃m當復制論文的部分或全部內容用于學術活動。學位論文作者簽名耕RA導■一。_。一一一。一。一一一一洲年，2，月；日經指導教師同意，本學位論文屬于保密，在年解密后適用本授權書。指導教師簽名學位論文作者簽名解密時間年月日各密級的最長保密年限及書寫格式規(guī)定如下內部5年最長5年，可少于5年秘密★10年最長10年，可少于10年機密★20年最長20年，可少于20年南開大學學位論文原創(chuàng)性聲明本人鄭重聲明所呈交的學位論文，是本人在導師指導下，進行研究工作所取得的成果。除文中已經注明引用的內容外，本學位論文的研究成果不包含任何他人創(chuàng)作的、已公開發(fā)表或者沒有公開發(fā)表的作品的內容。對本論文所涉及的研究工作做出貢獻的其他個人和集體，均已在文中以明確方式標明。本學位論文原創(chuàng)性聲明的法律責任由本人承J旦?！瘜W位論文作者簽名料歸孳年CY月弓EL

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簡介：單位代碼塑9Z學號塑Q】西北大學碩士學位論文題目國處墾查經渣查屋塑繾塑遢墼嬰塞經渣皇的魚廑作者_董凌指導教師笪鑒盛專業(yè)技術職務絲蕉學科專業(yè)絲盜塹答辯日期至QI學位授予日期二00七年六月ABSTRANSINCETHEREFORMANDOPENINGTHEDISTRIBUTIONANDSTRUCTUREOFSTATEOWNEDECONOMYHADCHANGEDALOTBECAUSEOFTHELONGBATTLELINE，THEWIDEDISTRIBUTIONAND●●UNREASONABLESTRUCTURE，WETOOKTHEEARLYA硒USTMENT雒GRANTEDTHEREASONWHYTHEECONOMICPROBLEMSSTILLCANNOTBESOLVEDQUICL【LYISBECAUSETHEREFORMISINASTAGEWHERECRITICALPROBLEMSNEEDTOBEADDRESSED，ANDOURKNOWLEDGEABOUTTHEDISTRIBUTIONANDADJUSTMENTTHEORYOFSTATEOWNEDECONOMYISNOTPROFOUNDTHEPAPERTRIESTEORGANIZETHEDISTRIBUTIONANDSTRUCNREA由USTMENTTHEORYOFSTATEOWNEDECONOMYINWHICHWEHOLDTHATTHERE’REATLEASTTHREEASPECTSOFPROBLEMSFIRSTLYTHEFLAWOFTHEORYLOGIC，WHICHMEANSTHEINCONFORMITYOFPREMISESHYPOTHESISINTHEPAPERWECONSIDERTHATBOTHGOVERNMENTANDTHEMASSPURSUEPRIVATEINTERESTSANDABANDONTHEPROUDICETHATGOVERNMENTANDTHEMASSKC印DIFFERENTPREFERENCESECONDLYTHECURRENTRESEARCHMAINLYFOCUSESONTHEPRIVATIZATIONMOVEMENTAFTERTHEWWNESPECIALLYINTHE1970SANDTHERESNOLONGTERMOBSERVATIONONTHEAPPEARANCE，DISTRIBUTIONOFSTATEOWNEDECONOMYTHIRDLYTHECURRENTRESEARCHINTRODUCESTHEREFORMEXPERIENCEOFDEVELOPEDDEVELOPINGORTRANSITIONALCOUNTRIESSEPARATELYANDTHERESNOUNIFIEDTHEORYWHICHCANEXPLAINDISTRIBUTIONANDSTRUCTUREADJUSTMENTOFTHEGLOBALSTATEOWNEDECONOMYBASEDOLLTHEABOVESUMUP，WEDIDLONGERANDRESEARCHANDMORETRUTHFULDESCRIPTIONABOUTTHEHISTORYOFDISTRIBUTIONANDSTRUCTUREADJUSTMENTOFSTATEOWNEDECONOMYANDSTRENGTHENEDOBSERVATIONONTHEEXPANSIONOFSTATEOWNEDECONOMYBASEDONNEWSUMUPANDANALYSISONTHEFEATURESOFTHEDISTRIBUTIONANDSTRUCTUREADJUSTMEUTOFGLOBALSTATEOWNEDECONOMYWEPROMOTETHATTHEORYLIKE“STANDARDIZEDINDUSTRIALIZATION’’AND“TRANSPOSITIONOFGOVERNMENTALFUNTION”SHOULDBEEXPLAINEDTHECONCLUSIONOFUSISTHATTHEDISTRIBUTIONOFSTATEOWNEDECONOMYSHOULDBECONSISTENTWITHGOVERNMENTALFUNCTIONSWEALSOPROVIDERELATEDSUGGESTIONSKEYWORDSSTATEOWNEDECONOMY；STATEOWNEDENTERPRISES；DISTRIBUTION；STMCTUREADJUSTMENTIII

簡介：無錫開拓動力有限公司亞洲市場國際營銷戰(zhàn)略的研究THERESEARCHOFWUXIKITOOPOWERCOLTDFMARKETINGSTRATEGYINASIANMARKETS學校上海交通大學學院安泰經濟與管理學院專業(yè)工商管理（MBA）作者楊衛(wèi)導師奚俊芳學號1061209385班級M0612092答辯日期2009年1月11日THERESEARCHOFWUXIKITOOPOWERCOLTDFMARKETINGSTRATEGYINASIANMARKETSABSTRACTWITHTHEFASTGROWTHOFWLDECONOMYINTHEPASTYEARSTHEWLDPOWERCONSUMPTIONGREWATTHESAMEEVENFASTERPACEWUXIKITOOPOWERCOLTDAMANUFACTUREROFSMALLANDMEDIUMCAPACITYGENERATSETSSEIZEDTHISOPPTUNITYANDSOONBECAMETHEBIGGESTMANUFACTURERAROUNDTHEWLDINSOMEFIELDSWITHANAVERAGEANNUALGROWTHOFAROUND60INTHELASTFOURYEARSHOWEVERTHEOUTBREAKOFSUBPRIMARYCRISISIN2007HADLEDTOAGLOBALFINANCIALSTMINSEPTEMBEROF2008WHICHCASTACLOUDOVERTHEFUTUREOFWLDECONOMYOVERSEASDEMANDFELLSHARPLYANDWLDMARKETSBECAMEMUCHMEUNCERTAINATTHISVERYMOMENTALLCHINESEGENERATSETSMANUFACTURERSINCLUDINGKITOOHAVETOTHINKOVERTHEFOLLOWINGQUESTIONSWHATWASTHEDRIVINGFCEFTHEHIGHSPEEDGROWTHINTHEPASTWHATWERETHEFACTSTHATINFLUENCEDTHEDEMANDHOWCANWEFINDTHE“NEXTRISINGSTAR”AMONGALLOVERSEOUNTRIESWITHTHEINTENTIONTOANSWERALLTHESEQUESTIONSTHEAUTHDOESHISRESEARCHBYUSINGTHEFOLLOWINGMETHODSFIRSTLYANALYZESANDFINDSOUTTHEFACTSTHATAFFECTGENERATSETSMARKETSECONDLYANALYZESTHEMACROANDMICROENVIRONMENTOFKITOOTHIRDLYBUILDSAMARKETATTRACTIONASSESSMENTMODELBASEDONTHEABOVEFACTSANDUSESTHEMODELTOEVALUATEALLASIAN

簡介：東北財經大學碩士學位論文亞洲金融危機的回顧反思與經驗總結姓名孟嘯宇申請學位級別碩士專業(yè)產業(yè)經濟指導教師段鵬飛20071101的不恰當措施。從對歷史事件和經濟環(huán)境的分析中，來歸納經濟危機發(fā)生的原因和條件。最后提出亞洲金融危機留給我們的經驗教訓和啟示。關鍵字；金融危機，泡沫經濟，金融監(jiān)管，聯(lián)系匯率，金融自由化Ⅱ

簡介：廈門大學碩士學位論文從東亞華商網絡的視角看“亞洲三小”經濟發(fā)展姓名張麗華申請學位級別碩士專業(yè)國際關系指導教師莊國土20080401ABSTRACTTHISPAPERISTITLEDONTHEECONOMICDEVELOPMENTOFHONGKONGTAIWANANDSINGAPOREFROMTHEPERSPECTIVEOFETHNICCHINESEBUSINESSNETWORKSINEASTASIATHEREARETWOTRADITIONALAPPROACHESTOTHESTUDYOFETHNICCHINESEBUSINESSNETWORKS，THEPOLITICALANDHISTORICALONESINTHISPAPERTHEAUTHORADDSTHEECONOMICAPPROACHTOTHEABOVEONESBESIDES，THEAUTHORCHOSESHONGKONG，TAIWANANDSINGAPOREASTHEOBJECTIVES，INORDERTOFIGUREOUTTHERELATIONSBETWEENTHEIRECONOMICDEVELOPMENTANDETHNICCHINESEBUSINESSNETWORKSTHEREARETHREEPARTSINTHISPAPERTHEINTRODUCTION，THESUBJECTANDTHEENDINGPARTTHEOUTLINEOFTHESUBJECTISASFOLLOWCHAPTERTWOISRIFLEDTHEOVERVIEWOFETHNICCHINESEBUSINESSNETWORKSINEASTASIAIT’SMAINLYABOUTTHEDEVELOPINGPROCESSOFETHNICCHINESEBUSINESSNETWORKSINEASTASIAPARTICULARLYINHONGKONG，TAIWANANDSINGAPORECHAPTERTHREEISTITLEDSINGAPORE’SECONOMICDEVELOPMENTANDETHNICCHINESEBUSINESSNETWORKSINEASTASIATOILLUSTRATETHERELATIONSBETWEENTHETWO，THEAUTHORINSPECTSINTOSINGAPORE’STRADEANDINVESTMENTBOTHINHORIZONTALANDLONGITUDINALWAYCHAPTERFOURISTITLEDHONGKONG’SECONOMICDEVELOPMENTANDETHNICCHINESEBUSINESSNETWORKSINEASTASIATHEAUTHORANALYSESHONGKONG’STRADEANDINVESTMENTBYPERIODS，ANDITSTRANSITTRADEISTHEHIGHLIGHTCHAPTERFIVEISTITLEDTAIWAN’SECONOMICDEVELOPMENTANDETHNICCHINESEBUSINESSNETWORKSINEASTASIA，WHICHISALSOABOUTTHETRADEANDINVESTMENTTHETRAITSOFTAIWAN’SECONOMYISSTRESSEDLAST，THERELATIONSBETWEENETHNICCHINESEBUSINESSNETWORKSANDEACHECONOMYAREEMPHASIZEDINTHEENDOFEACHCHAPTERKEYWORDSETHNICCHINESEBUSINESSNETWORKS；EASTASIA；NIES

簡介：湖南大學碩士學位論文中國對亞洲地區(qū)貿易逆差問題研究姓名李艷婕申請學位級別碩士專業(yè)國際貿易學指導教師張亞斌20071008中國對亞洲地區(qū)貿易逆差問題研究ABSTRACTINRECENTYEARS，FOREIGNTRADEINCHINAHASBEINGDEVELOPEDQUICKLY，BUTALSOWITHLARGEAMOUNTOFUNBALANCE．AMONGTHEM,WHENMENTIONEDTHETRADINGBETWEENCHINAANDOTHERASIANCOUNTRIESANDREGIONS，THEAMOUNTOFUNFAVORABLEBALANCEISHUGEANDWITHTHETRENDTHATTHISUNBALANCEFIGUREISBECOMINGBIGGERALONGWITHTHEDEEPDEVELOPMENTOFTRADINGBETWEENCHINAANDOTHERNATIONSANDREGIONSINASIA．ASANIMPORTANTTRADINGCOMPANIONWITHCHINA,ITISCRUCIALTOANALYZETHESITUATIONINTRADINGBALANCEANDTHEREASONOFUNBALANCE．BASEDONTHIS，MAKINGTHECORRECTPOLICYINTRADINGISOFPROFOUNDMEANING．THEARTICLEFIRSTLYBEGANWITHTHEPRESENTTRADINGCONDITIONSBETWEENCHINAANDOTHERASIAREGIONS，SHOWINGTHECHARACTERISTICOFUNFAVORABLEBALANCEBYTHEWAYOFTOTALAMOUNTANDSTRUCTUREANALYZE．THEN,USINGTHEMETHODOFTHEORETICALANDEMPIRICALANALYSISSEARCHEDTHEREASONOFUNFAVORABLEBALANCEINTHEASPECTOFBOTHECONOMYFACTORSANDTHEPOLICYSYSTEMSECONOMYGROWTHRATE，FDI，EXCHANGERATEANDPROTECTIONPOLICYINTRADEBY,SOMECOUNTRIESALLDOCONTRIBUTIONTOTHEUNFAVORABLEBALANCE．INTHEEND,THEARTICLEHASGIVENANEVALUATIONTOTHEUNFAVORABLEBALANCEOFTRADEANDALSOGIVENSOMESUGGESTIONSANDABLUEPICTUREOFTRADINGINASIA．THENNFAVORABLEBALANCEWOULDSTILLBEEXISTEDANDTHEAMOUNTWOULDBECOMEBIGGER．FOROURCOUNTRY,WITHTHEOPPORTUNITYOFFASTDEVELOPMENTOFECONOMY，WESHOULDUPDATETHEIMPORTANDEXPORTSTRUCTUREOFMERCHANDISES,IMPROVETHETECHNOLOGYCONTENTINEXPORTCOMMODITIES．CHANGINGTHEEXPORTMERCHANDISESFROMLOWADDITIONALVALUETOHIGHADDITIONALVALUEISTHEONLYDEVELOPMENTROUTEWHENTRADINGWITHOUTSIDE．INTHEMEANTIME，WESHOULDACCELERATETHEPROGRESSINSIGNINGFREETRADEAGREEMENTSWITHNEIGHBORINGCOUNTRIES，PROMOTETRADELIBERALISMANDCREATEAGOODENVIRONMENTFORTHEFREETRADEINTHEREGIONANDTHEWHOLEWORLD．KEYWORDSUNFAVORABLEBALANCEOFTRADE；FOREIGNDIRECTINVESTMENT；ECONOMYGROWTHRATE；STRUCTUREOFINDUSTRY；EXCHANGERATEHI

簡介：分類號河南農業(yè)大學碩士學位論文密級英文題目CLONINGANDEXPRESSIONOFTHREELOWMOLECULARWEIGHTANTIGENGENESOFCVSTICERCUSCELLILOSAEANDNESELLTLOSAEAN0■______一IIESTABLISHMENTOFELISAUSINGRECOMBINANTPROTAIN導專論文提交日學位授予日期致謝本論文是在導師寧長申教授、張改平研究員和張龍現教授的悉心指導下，在河南省農業(yè)科學院河南省免疫學重點實驗室完成的。衷心感謝三位恩師三年來在生活中無微不至的關心，在實驗過程中耐心細致的指導和幫助。老師們對科學問題的高瞻遠囑，淵博的學識、嚴謹的治學態(tài)度，以及求真務實、勇于創(chuàng)新、不懈追求的精神，使我終身受益，永遠是我學習的典范和努力方向。三年的求學過程中，我深切感受到導師無微不至的關懷和愛護，感激之情難于言表，我將永遠銘記在心。感謝河南省農科院河南省免疫學重點實驗室給我的科研工作提供了優(yōu)良的科研條件。張改平老師博大精深的學識、寬廣的視野、強烈的科學精神深深感染著我，使我受益匪淺。衷心感謝周玲書記、鄧瑞廣主任、王選年教授、楊艷艷研究員、李學伍副研究員、肖治軍副研究員、羅俊博士、郝慧芳博士、王麗碩士在工作和生活中給予的熱情指導和幫助。感謝張紅、喬松林、趙緒永、席俊和楊蘇珍博士；感謝李嬌、朱禮倩、李清洲、彩鴻翔、劉運超、田曉輝、李喬木、游雷鳴、馮麗麗、張麗萍、張麗娜、劉宣兵、李卓洋、萬博、苗獻偉、鄔成業(yè)、史平玲、卜丹、祁艷華等在試驗中給我的無私熱情的幫助和支持。衷心感謝菅復春副教授、劉紅英老師、張素梅老師三年來在學習和生活上給予的關懷和幫助。感謝師姐魯琨、孫艷平、朱靜靜、王芳，師兄王榮軍、趙光輝、崔彬、呂彪、仇書興、李家誠、董和平、胡群山、黨海亮、馬超峰、王永立在生活、學習和實驗中給予的無私幫助和開導。感謝段張秀、楊紅玉、史柯、任慶娥、李勉、周春香、高庚渠、呂超超、王巖、周洋、齊萌等寄生蟲學與寄生蟲病學實驗室全體同學在試驗中提供的方便和幫助。感謝導師組崔保安教授、張改平研究員、王川慶教授、夏平安副教授，李文剛教授、許蘭菊教授、陳陸副教授、陳麗穎副教授、陳紅英副教授、張紅英副教授及其他各位老師在完成學習任務和課題研究中對我的指導和幫助。最后，感謝我的父母，感謝我的愛人馬景周和我的孩子馬睿苑，是他們的愛和理解及支持減少了我的后顧之憂，使得我能夠靜下心來完成我這三年來的學習和科研工作。真誠感謝所有關心、支持和幫助我的老師、同學和親友。

簡介：安徽農業(yè)大學碩士學位論文亞洲璃眼蜱HBP基因的克隆表達及其活性研究姓名李莊申請學位級別碩士專業(yè)預防獸醫(yī)學指導教師李培英周金林20090601不同解剖部位包括殼、唾液腺和腸的總RNA，取相同量的總RNA進行RTPCR分析，結果表明，該基因的表達具有性別特異性，在蜱的各個發(fā)育階段僅吸血的雌蜱表達；且HAHBP基因在亞洲璃眼蜱半飽血雌蜱的唾液腺、殼及中腸這三個器官中均高效表達。再次，構建了重組表達質粒載體，并轉化表達宿主菌BL21DE3，經IPTG誘導，結果表明HAHBP可以在原核表達系統(tǒng)中高效表達，應用GST純化系統(tǒng)，可獲得較純的目的蛋白，WESTERNBLOT結果表明原核表達的融合蛋白具有很好的免疫原性。最后，以純化得到的融合蛋白GSTHAHBPGST蛋白作陰性對照作為抗原包被于ELISA酶標板4℃過夜，洗滌后，加入HISTAMINEHRP酶結合物37。C反應2H，充分洗滌后，加入LOBLUE底物顯色10RAIN后，加入終止液5MIN，酶標儀測定OD值。首次證明了HAHBP具有結合組胺的活性。綜上所述，本研究成功地克隆到亞洲璃眼蜱組胺結合蛋白基因；經原核表達的重組蛋白具有良好的免疫原性，為抗亞洲璃眼蜱疫苗的研制提供了新的候選抗原分子，對蜱及蜱傳疾病的防治具有重要意義。關鍵詞亞洲璃眼蜱，組胺結合蛋白，克隆表達，活性Ⅱ