非洲爪蟾睪丸緊密連接蛋白occludin和claudin-11的表達及功能分析.pdf

1、浙江大學生命科學學院碩士學位論文非洲爪蟾睪丸緊密連接蛋白occludin和claudin11的表達及功能分析姓名：陳芝娟申請學位級別：碩士專業(yè)：細胞生物學指導教師：楊萬喜20091120浙江大學碩上學位論文非洲爪蟾睪丸緊密連接蛋白occludin和claudin一11的表達及功能分析AbstractSpermatogenesisisaverycomplicatedprocess，whichiscloselyrelatedtotheblo

2、od—testisbarrier(BTB)inmammalsBTBiscomposedbycontinuoustightjunctionsbetweenadjacentSertolicellsatthebasalcompartmentoftheseminiferoustubuleItisanimportantphysiologicalstructurethatcanprotectgermcellsfromautoimmunesystem

3、ofhostandharmfulmoleculesinbloodAndalSOtheBTBiSanaturalbarrierthatcreatesasuitablemicroenvironmentforthedevelopmentofgermcellsbykeepingoutproteinsandothermacromoleculesAmongtheintegralcomponentproteins，occludinandclaudin

4、11areessentialforthestructureandfunctionoftightjunctionsThisprogramtakesAfricanfrog(Xenopuslaevis)asthemodelandReversetranscription—PCRandWesternblotwereperformedtoconfirmtheexpressionofoccludinandclaudin一11inXenopustest

5、is；Theimmnofluerescencewasdonetostudythelocalizationofthesetwoproteins；additionallyQuantitativeRealtimePCRisalsoemployedtoanalyzeandcomparethemRNAexpressionlevelofoccludinandclaudin11inbothpreandpostmatureXenopustestisHi

6、ghexpressionlevelofthesetwoproteinsaredetectedinXenopustestisNosignificantdifferencesintheirmRNAexpressionweredetectedbetweenpreandpostmatureXenopustestisTheresultspartlymatchesourpredictionthatoccludinandclaudin一11would

7、expressabundantlyandsteadilyandmaybeittoldUSthatthesetwoproteinsCanplaysomefunctionalrolesastheydidintheBTBinmammalstestis，providingasafeenvironmentforthespermatogenesisButfurtherexperimentsneedtobedonetoelucidatetheexis