LI-cadherin基因表達(dá)水平的變化對(duì)胃癌細(xì)胞生物學(xué)性狀的影響研究.pdf

1、石河子大學(xué)碩士學(xué)位論文LI-cadherin基因表達(dá)水平的變化對(duì)胃癌細(xì)胞生物學(xué)性狀的影響研究姓名：奚海林申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：外科學(xué)指導(dǎo)教師：牛建華201004012Abstract Objectives： The incidence rate of gastric carcinoma is also in the first position among manlignant tumors in our country,and th

2、e mortality is about one quarter of global ones.Most patients have been progressing to advanced stage at the time of diagnosis. The rate of radical resection is lower, treatments including radiation, chemotherapy or preo

3、perative chemotherapy have little infuence,even exert great side effect to patients.And at present having no normative chemotherapy, therefore the gene therapy has been introduced and displayed its promising. Two importa

4、nt properties of cancers are able to deregulate cell proliferation and suppress cell death. liver-intestine cadherin(CDH17) as one protein which have intimate correlation with invasion and metastasis of tumor,which as we

5、ll as highly expresses in intestinalmucosa.This study extracted and identified CDH17/pcDNA3.1(-) eukaryotic expressing plasmid, and transfected it into BGC-826 cell by Lipofectmine 2000, the purpose is observing whether

6、 the extraneous CDH17 gene can transfect BGC-826 effectively as a anti- tumor factor. It provides the experimental foundation for further studying that transfection of this plasmid alone or combined with other plasmids t

7、o block the stomach neoplasms in different steps. Method： 1 Transient transfection and expression of gastric tumor cells Gastric carcinoma BGC-826 cells were transfected with Lipofectamine 2000.Protein of cells were ext

8、racted at the time of 48h after tranfected, the method of Western blot was used to identify the expression of CDH17. 2 The assays after transient transfection in vitro The assays were divided into three groups ,The cell

9、s transfected by CDH17/pcDNA3.1(-) was experimental groups,the cells transfected by pcDNA3.1(-) was blank control and BGC-826 cells untransfected was negative control. Cell matrigel invasion assay, scratching assay were

10、used to study the effects of CDH17 gene. Results： 1 Transient expression of CDH17. after transfected.After transfeted 48h, protein of CDH17 was detected with Western blot. 2 All assays showed that the invasiveness an

11、d migration of BGC-826 cells transfected by CDH17/pcDNA3.1(-) were less than those of BGC-826 cells transfected by pcDNA3.1(-) and untransfected cells. Conclusion： CDH17 has been successfully transfected, they can be exp

12、ressed in the gastric carcinoma cells.All assays suggest that CDH17 can affect biological characters of human gastric carcinoma cell line BGC-826. They may depress the invasion and metastasis of gastric carcinoma cell. L

13、ocal cancer violations or distant metastasis often lead to treatment failure of the main reasons, the incidence of gastric cancer on the molecular mechanism of the biological behavior and the search for new anti-transfer