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1、西南農(nóng)業(yè)大學(xué)碩士學(xué)位論文青稞β葡聚糖的分離純化及其理化性質(zhì)研究姓名：張峰申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：食品科學(xué)指導(dǎo)教師：劉雄闞建全20050501AbstractHighlandbarley(ttordoumVMgareLVal“nudumHook力isakindofcharacteristiccropsinTibetregionConsiderableattentionhasbeenfocusedOilitforitsabundant13嘻

2、ucanInthispaper，B召UCanhasbeenextractedbymeansofhotwaterwithhighlandbarleybranasrawmaterialsBasedOnthesinglefactorexperiment，technologicalparametersofextractionhasbeendefinedandtheeffectsoffactorsonextractionhasbeendescri

3、bedTbefeasibilityofexperimentresultshasbeenvedfiedaA6glucanWasobtainedfromcrude6一glueanbyremovalofprotein，precipitationbyethanol’ammoniumsulfatefraetionation，dialysis，cellulosecolumnchromatographyandSephadexG一100columnch

4、romatographyeTheoptimizedconditionsofprecipitationbyethanolandcolumnchromatographyhavebeendeterminedPurityandphysicoehemiealpropertyofABglucanhasalsobeendeterminedandanalyzed。Theresultsshowedasfollows1ThecontentofBglucan

5、ishighestinhighlendbarleybran(578％)Itindicatedthatl3glueanwasfoundmainlyinaleuronelayerofhighlandbarley2Theeffectofpretreatmant(dryingwithhightemperatureandethanolrefluxing)onextractionofBglucanwasnotsignificant3Theorder

6、ofmajorandsecondaryfactorsinfluencingBOUCanextractionWaspH，temperature，timeandstockratioTheoptimumextractionconditionswere：pH9，50“C，time2h，stockratiol；15，andtwotimesofextraction。TheyieldfrombarleybranWas665％Thepurifiedpr

7、eparationcontained6472％Bgluean4ThepurificationprocessofBglueanwasconfirmed：removalofproteinbyadjustingpH，ammoniumsulfatefractionation，precipitationbyethan01Bglucanwith887％purityWasobtainedThencellulosecolumnchromatograph

8、yandSephadexG100columnchromatographyWasusedtoisolateitandA8gl喊withanalogmtsmolecularweightWasgainedItsratioswas045％5，MonosaccharideofA8一glueanWSSmadeofglucose1dolec‘ilarⅥ盱igIltofsampleAWas3010’DaiSampleWascomposedofgluco

9、sylbondSampleAwasinsolubleinethanol，aetherandacetoneItsviscositieswas42mPaS【a1一(H20)was一953Theyhadnocolorreactionwithpotassiumiodide。whichexplainedthattheyWerononstarchpolysaccharideTheirreactionwithFehlingreagents，biure

10、treagents，ferricchloridereagentsCTABreagentsandtheirabsorptionin280rimwerenegativeTheseresultsshowedthattheydidnotcontainmonosaceharide，protein，polyphenolsandnucleicacidandtheywereneutralpolysaecharideKeywords：Highlandba

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