基于磁球放大技術(shù)的電化學(xué)發(fā)光免疫分析研究.pdf

1、山東大學(xué)碩士學(xué)位論文基于磁球放大技術(shù)的電化學(xué)發(fā)光免疫分析研究姓名：陳莉申請學(xué)位級別：碩士專業(yè)：分析化學(xué)指導(dǎo)教師：金文睿20080526山東大學(xué)碩士學(xué)位論文ABSTRACTInthechapterone，Rubis(2，2bipyridine)(2，2bipyridine一4，4dicarboxylicacid)N—hydroxysuccinimideester(Ru(bpy)2(dcbpy)NHS)wassynthesizedandch

2、aracterizedusinginfraredspectroscopyultravioletvisibleabsorptionspectroscopyfluorescencespectroscopyandelectrochemiluminescence(ECL)spectroscopyAmaximumappearedat672nmontheECLspectrumofRu(bpyh(dcbpy)NHSInordertofabricate

3、themagneticbeadsmodifiedwithRu(bpyh(dcbpy)NHSbiotincoatedmagneticbeads(bioMB)orstreptavidin—coatedmagneticbeads(SA—MB)wasusedTheECL&themagneticbeadsmodifiedwasinvestigatedInthechaptertwo，wedevelopedanewECLimmunoassaymeth

4、odtodetectcancerantigen125(CAl25)usingthemagneticbeadsmodified、析thRu(bpyh(dcbpy)NHSInthismethod，theprimaryantibody(Abl)wasimmobilizedonthesinalizedtransparentindiumtinoxide(ITO)electrodeThen，thetargetantigen(Ag，CAl25)was

5、boundtoAblthroughtheimmunoreaction，followedbyconjugatingthebiotinylatedsecondaryantibody(Ab2)toAgSubsequenflNthestreptavidin(SA)wasconjugatedtotheAb2，followedbybindingbioMBtoSA。Finall3；Ru(bpy)2(dcbpy)NIISwaslabeledtothes

6、urfaceoftheMBsTheECLspectrumofthesystemfabricatedusingdifferentAgconcentrationswasrecordedThemaximumoftheintensityontheECLspectrawasusedtoquantifyAgThelinearrangeofthemethodwas5OOxl0一mol／L～100x10～mol／LforCA125Keywords：el