GP-7對人舌鱗癌Tca-8113細胞體外轉(zhuǎn)移潛能的影響.pdf

1、 原 創(chuàng) 性 聲 明 本人鄭重聲明： 本人所呈交的學位論文， 是在導師的指導下獨立進行研究所取得的成果。學位論文中凡引用他人已經(jīng)發(fā)表或未發(fā)表的成果、數(shù)據(jù)、觀點等，均已明確注明出處。 除文中已經(jīng)注明引用的內(nèi)容外， 不包含任何其他個人或集體已經(jīng)發(fā)表或撰寫過的科研成果。對本文的研究成果做出重要貢獻的個人和集體，均已在文中以明確方式標明。 本聲明的法律責任由本人承擔。 論文作者簽名：

2、 日 期： 關于學位論文使用授權(quán)的聲明 關于學位論文使用授權(quán)的聲明 本人在導師指導下所完成的論文及相關的職務作品，知識產(chǎn)權(quán)歸屬蘭州大學。本人完全了解蘭州大學有關保存、使用學位論文的規(guī)定，同意學校保存或向國家有關部門或機構(gòu)送交論文的紙質(zhì)版和電子版， 允許論文被查閱和借閱； 本人授權(quán)蘭州大學可以將本學位論文的全部或部分內(nèi)容編入有關數(shù)據(jù)庫進行檢索， 可以采用任何復制手段保存和匯編本學位論文。 本人離校后發(fā)表、 使

3、用學位論文或與該論文直接相關的學術論文或成果時，第一署名單位仍然為蘭州大學。 本學位論文研究內(nèi)容： □可以公開 □不宜公開，已在學位辦公室辦理保密申請，解密后適用本授權(quán)書。 （請在以上選項內(nèi)選擇其中一項打“√”） 論文作者簽名： 導師簽名： 日 期： 日 期：

4、 II Effects of GP-7 on the metastasis potential of Human tongue carcinoma Tca-8113 cells in vitro Abstract Objective: 4-[4″-(2″, 2″, 6″, 6″-tetramethyl-1″- piperidinyloxy nitroxide radical) amino] -4′-demethyl epipodopHy

5、llotoxin (GP-7) is one of semi-synthesized derivatives of nitroxide-introduced pHyllotoxin, which has lower toxicity and higher activity. It has been reported that GP-7 has significant anti-tumor effects on many kinds of

6、 cancer. However, the effects and mechanisms of GP-7 on Tca-8113 cells’ proliferation, invasion, migration and adhesion viabilities have not been reported until now. In the study, we investigated the effects and mechanis

7、m of GP-7 on Tca-8113 cells proliferation and metastasis in vitro. Methods: The proliferation viability of Tca-8113 cells treated with GP-7 was measured using Sulforhodamine B (SRB) assay. The viabilities of Tca-8113 cel

8、l’s invasion and migration were evaluated by Transwell chamber migration assay. The viability of Tca-8113 cell’s adhesion was assayed by cell-matrix adhesion assay. The protein expression levels were determined by Wester

9、n blotting analysis. The MMP-2 and MMP-9 levels were determined by Gelatin ZymograpHy assay. The NF-κB P65 nuclear transferation and activation capacity were determined using Nuclear Translocation Assay Kit. Results: The

10、 SRB assay showed that GP-7 25μg/mL significantly inhibited Tca-8113 cell proliferation. The inhibition rates of GP-7 in 24h, 48h, and 72h respectively were 67.49%, 70.58%, and 94.40%, respectively. GP-7 25μg/mL obviousl

11、y inhibited Tca-8113 cell invasion, migration and adhesion in 24h, respectively and their inhibition rates were 84.89%, 79.16%, 58.69%, respectively. Moreover, the expressions of p-Akt and p-IKK were reduced after treate