非洲爪蟾睪丸緊密連接蛋白o(hù)ccludin和claudin-11的表達(dá)及功能分析.pdf

1、浙江大學(xué)生命科學(xué)學(xué)院碩士學(xué)位論文非洲爪蟾睪丸緊密連接蛋白o(hù)ccludin和claudin11的表達(dá)及功能分析姓名：陳芝娟申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：細(xì)胞生物學(xué)指導(dǎo)教師：楊萬(wàn)喜20091120浙江大學(xué)碩上學(xué)位論文非洲爪蟾睪丸緊密連接蛋白o(hù)ccludin和claudin一11的表達(dá)及功能分析AbstractSpermatogenesisisaverycomplicatedprocess，whichiscloselyrelatedtotheblo

2、od—testisbarrier(BTB)inmammalsBTBiscomposedbycontinuoustightjunctionsbetweenadjacentSertolicellsatthebasalcompartmentoftheseminiferoustubuleItisanimportantphysiologicalstructurethatcanprotectgermcellsfromautoimmunesystem

3、ofhostandharmfulmoleculesinbloodAndalSOtheBTBiSanaturalbarrierthatcreatesasuitablemicroenvironmentforthedevelopmentofgermcellsbykeepingoutproteinsandothermacromoleculesAmongtheintegralcomponentproteins，occludinandclaudin

4、11areessentialforthestructureandfunctionoftightjunctionsThisprogramtakesAfricanfrog(Xenopuslaevis)asthemodelandReversetranscription—PCRandWesternblotwereperformedtoconfirmtheexpressionofoccludinandclaudin一11inXenopustest

5、is；Theimmnofluerescencewasdonetostudythelocalizationofthesetwoproteins；additionallyQuantitativeRealtimePCRisalsoemployedtoanalyzeandcomparethemRNAexpressionlevelofoccludinandclaudin11inbothpreandpostmatureXenopustestisHi

6、ghexpressionlevelofthesetwoproteinsaredetectedinXenopustestisNosignificantdifferencesintheirmRNAexpressionweredetectedbetweenpreandpostmatureXenopustestisTheresultspartlymatchesourpredictionthatoccludinandclaudin一11would

7、expressabundantlyandsteadilyandmaybeittoldUSthatthesetwoproteinsCanplaysomefunctionalrolesastheydidintheBTBinmammalstestis，providingasafeenvironmentforthespermatogenesisButfurtherexperimentsneedtobedonetoelucidatetheexis