南強菌素高產(chǎn)菌株選育與培養(yǎng)基優(yōu)化.pdf

1、目錄摘要..........……，..............................................................……lAbstraCt......................……二................................................……!前言....................................................

2、.............................……11海洋天然產(chǎn)物的研究...........................................................................……22海洋微生物次級代謝產(chǎn)物的研究進展.................................................……83海洋真菌次級代謝產(chǎn)物的研究進展...............

3、....................................……103.1海洋真菌...........................................................................................................……103.2海洋真菌抗腫瘤活性代謝產(chǎn)物...........……，................................

4、.....................……n4紅樹植物內(nèi)生真菌及其次級代謝產(chǎn)物...............................................……144.1紅樹植物及紅樹植物內(nèi)生真菌.......................................................................……144.2紅樹植物內(nèi)生真菌次級代謝產(chǎn)物的研究.................

5、......................................……巧4.3紅樹內(nèi)生真菌A123及其次級代謝產(chǎn)物的研究186!.........................................……195高產(chǎn)菌種選育....................................................................................……205.1誘變育種....

6、.......................................................................................................……215.2育種工作高通量篩選方法...............................................................................……266培養(yǎng)基優(yōu)化..........

7、..............................................................................……287本課題研究目的、內(nèi)容和意義..........................................................……31材料與方法.........................................................

8、.............……321材料.................................................................................................……322方法................................................................................................

9、.……37結(jié)果與分析......................................................................……481菌株A123形態(tài)觀察............................................................................……481.1A123菌株的誘導(dǎo)產(chǎn)抱...............................

10、........................................................……481.2A123抱子OAPI染色觀察.................................................................................……481.3A123菌絲OAPI染色觀察.........................................

11、........................................……492原生質(zhì)體誘變條件優(yōu)化......................................................................……S02.1原生質(zhì)體制備......................................……，.........................................

12、.............……502.2原生質(zhì)體再生....................................................................................................……502.3原生質(zhì)體誘變...................……，.……，.，.....................................................

13、...........……503南強菌素高產(chǎn)突變株篩選方法的建立...............................................……SZ3.1依據(jù)表型差異的突變株篩選...........................................................................……523.2依據(jù)抗菌活性差異的突變株篩選..........................

14、..........................................……543.3依據(jù)TLc差異的突變株篩選............................................................................……553.4依據(jù)HpLC的突變株篩選...............................................................

15、...................……563.5質(zhì)譜(ESI一MS)................................................................................................……613.6抽NMR.............................................................................

16、..................................……613.7“抗菌活性一TLC一HPLC”聯(lián)用篩選方法確立...................................................……62I斤CatalogueAbst門CtinChinGSGAbstractinEnglish.........................……二...........................……

17、l!ntrdUCtiOn.........................................................................……1，Advanceonmarinenaturalproducte...........................................……22Advanceonsecondarymetabolitesfrommarinemicroorganism…8

18、3Advanceonsecondarymetabolitesfrommarinefungi............……103.1Marinefungi...............................……，..................................................……，o3.2Anti一tummetabolitesfrommanrinefungi...................

19、...............……114AdvaneeonmetaboIiteSfrommangroveendoPhyticfungi......……144.，MangroveandmangroveendophytiCfungi..................................……144.2SeeondarymetaboliteSfrommangroveendoPhytiefungi.........……154.3Se

20、COndarymetabolitesofendoPhytiefongusA123...................……195StrainSeleCtiOn.............................................................................……205.，Mutetionbreeding.........................................

21、.........................................……215.2HighThroughpUtScreening...................................................................……266Optimizati0n0fculturemediem..................................................

22、……287purpose，contentaandsigni打cance...........................................……3，MaterialSandmethodS..................................................……321MaterialS.......................................................

23、.................................……322MethOdS.........................................................................................……37Resulteandanalysis......................................................……481M0rph

24、0us0fstrainA123..............................................................……481.，Sporeinduced..................................................……，..............................……481.2Sporedyeing.......................

25、..................................................................……48，.3MyceliUmdyeing...................................................................................……492Optimizati0n0fpr0t0plastamutagenesis.........

26、.........................……502.1尸reparation............................................................................................……502.2Regenarati0n..................................................................

27、......................……502.3Mutagenesis..........................................................................................……503EstablishofHighThroughPutScreeningmethod.....................……523.，SCreeningdepentde

28、ntOnphenofypeofmutants...................................……523.2Screeningdepentdentonantimicrobial日ctMty.....................................……543.3ScreeningdepentdentonTLC................................................

29、...............……553.4Screeningdepentdenton日尸LC........................……，.............................……563.5ES!一MS..................................................................................................……613.